{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T10:35:52Z","timestamp":1766486152692},"reference-count":38,"publisher":"Informa UK Limited","issue":"3","license":[{"start":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T00:00:00Z","timestamp":1425427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Korean Health Technology R&D Projects","award":["R1306922"],"award-info":[{"award-number":["R1306922"]}]},{"name":"Ministry for Health & Welfare, Republic of Korea (O.S.S), and by the Cooperative Research Program for Agriculture Science & Technology Development","award":["PJ009990032014"],"award-info":[{"award-number":["PJ009990032014"]}]},{"name":"Rural Development Administration, Republic of Korea (S.Y.J.)"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In this study, we investigated antibacterial activities of 20 plant-derived natural compounds against Gram-negative enteric pathogens. We found that both flavonoids and non-flavonoids, including honokiol and magnolol, possess specific antibacterial activities against V. cholerae, but not against other species of Gram-negative bacterium which we tested. Using various antibacterial assays, we determined that there was a dose-dependent bactericidal and biofilm inhibitory activity of honokiol and magnolol against Vibrio cholerae. In addition to antibacterial activities, these molecules also induced an attenuating effect on reactive oxygen species (ROS) production and pro-inflammatory responses generated by macrophages in response to lipopolysaccharides (LPS). Additionally, Caenorhabditis elegans lethality assay revealed that honokiol and magnolol have an ability to extend a lifespan of V. cholerae-infected worms, contributing to prolonged survival of worms after lethal infection. Altogether, our data show for the first time that honokiol and magnolol may be considered as attractive protective or preventive food adjuncts for cholera.<\/jats:p>","DOI":"10.1080\/09168451.2014.991685","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T11:00:01Z","timestamp":1418814001000},"page":"475-483","update-policy":"http:\/\/dx.doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":17,"title":["<i>In vitro<\/i> and <i>in vivo<\/i> antimicrobial efficacy of natural plant-derived compounds against <i>Vibrio cholerae<\/i> of O1 El Tor Inaba serotype"],"prefix":"10.1080","volume":"79","author":[{"given":"Hyung-Ip","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji-Ae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun-Jin","family":"Choi","sequence":"additional","affiliation":[{"name":"Asian Pacific Influenza Institute, College of Medicine, Korea University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason B","family":"Harris","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA"},{"name":"Department of Medicine, Harvard Medical School, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong-Yeop","family":"Jeong","sequence":"additional","affiliation":[{"name":"Microbial Institute for Fermentation Industry (MIFI), Sunchang-gun, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok-Jun","family":"Son","sequence":"additional","affiliation":[{"name":"BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University, Jeonju, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Younghoon","family":"Kim","sequence":"additional","affiliation":[{"name":"BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University, Jeonju, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ok Sarah","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, Republic of Korea"},{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2015,3,4]]},"reference":[{"key":"2021033107574632100_CIT0001","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1016\/S0140-6736(12)60436-X","article-title":"Cholera","volume":"379","author":"Harris","year":"2012","journal-title":"Lancet"},{"key":"2021033107574632100_CIT0002","first-page":"241","article-title":"Emergence of multidrug-resistant strain of Vibrio cholerae O1 in Bangladesh and reversal of their susceptibility to tetracycline after two years","volume":"25","author":"Faruque","year":"2007","journal-title":"J Health Popul Nutr"},{"key":"2021033107574632100_CIT0003","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1056\/NEJMoa054493","article-title":"Single-dose azithromycin for the treatment of cholera in adults","volume":"354","author":"Saha","year":"2006","journal-title":"N Engl J Med"},{"key":"2021033107574632100_CIT0004","doi-asserted-by":"crossref","first-page":"357","DOI":"10.3109\/00498251003663724","article-title":"Baicalin, an emerging multi-therapeutic agent: pharmacodynamics, pharmacokinetics, and considerations from drug development perspectives","volume":"40","author":"Srinivas","year":"2010","journal-title":"Xenobiotica"},{"key":"2021033107574632100_CIT0005","doi-asserted-by":"crossref","first-page":"35501","DOI":"10.1074\/jbc.M302967200","article-title":"Honokiol, a small molecular weight natural product, inhibits angiogenesis in vitro and tumor growth in vivo","volume":"278","author":"Bai","year":"2003","journal-title":"J. Biol. Chem"},{"key":"2021033107574632100_CIT0006","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1182\/blood-2004-11-4273","article-title":"The natural product honokiol induces caspase-dependent apoptosis in B-cell chronic lymphocytic leukemia (B-CLL) cells","volume":"106","author":"Battle","year":"2005","journal-title":"Blood"},{"key":"2021033107574632100_CIT0007","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1055\/s-2005-864100","article-title":"Anti-inflammatory effects of magnolol and honokiol are mediated through inhibition of the downstream pathway of MEKK-1 in Nf-\u03bab activation signaling","volume":"71","author":"Lee","year":"2005","journal-title":"Planta Med"},{"key":"2021033107574632100_CIT0008","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ejphar.2004.05.047","article-title":"In vitro antibacterial and anti-inflammatory effects of honokiol and magnolol against Propionibacterium sp","volume":"496","author":"Park","year":"2004","journal-title":"Eur. J. Pharmacol"},{"key":"2021033107574632100_CIT0009","first-page":"1121","article-title":"Antimicrobial activity and cytotoxic effects of Magnolia dealbata and its active compounds","volume":"6","author":"Jacobo-Salcedo Mdel","year":"2011","journal-title":"Nat. Prod. Commun"},{"key":"2021033107574632100_CIT0010","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/ptr.736","article-title":"Antimicrobial activity of honokiol and magnolol isolated from Magnolia officinalis","volume":"15","author":"Ho","year":"2001","journal-title":"Phytother Res"},{"key":"2021033107574632100_CIT0011","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/BF02976465","article-title":"Antifungal activity of magnolol and honokiol","volume":"23","author":"Bang","year":"2000","journal-title":"Arch. Pharmacal Res"},{"key":"2021033107574632100_CIT0012","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.micinf.2012.02.007","article-title":"Effect of quercetin on Vibrio cholerae induced nuclear factor-\u03baB activation and interleukin-8 expression in intestinal epithelial cells","volume":"14","author":"Das","year":"2012","journal-title":"Microbes Infect"},{"key":"2021033107574632100_CIT0013","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.toxicon.2010.03.009","article-title":"Resveratrol, a natural polyphenolic compound, inhibits cholera toxin-induced cyclic AMP accumulation in Vero cells","volume":"56","author":"Morinaga","year":"2010","journal-title":"Toxicon"},{"key":"2021033107574632100_CIT0014","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1073\/pnas.052694299","article-title":"Quorum-sensing regulators control virulence gene expression in Vibrio cholerae","volume":"99","author":"Zhu","year":"2002","journal-title":"Proc. Nat. Acad. Sci. U.S.A"},{"key":"2021033107574632100_CIT0015","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.1128\/IAI.06350-11","article-title":"Caenorhabditis elegans immune conditioning with the probiotic bacterium Lactobacillus acidophilus strain NCFM enhances gram-positive immune responses","volume":"80","author":"Kim","year":"2012","journal-title":"Infect Immun"},{"key":"2021033107574632100_CIT0016","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1093\/jac\/dks184","article-title":"Wall teichoic acid protects Staphylococcus aureus from inhibition by Congo red and other dyes","volume":"67","author":"Suzuki","year":"2012","journal-title":"J. Antimicrob. Chemother"},{"key":"2021033107574632100_CIT0017","doi-asserted-by":"crossref","first-page":"163","DOI":"10.4321\/S1137-66272005000300002","article-title":"Bacterial biofilms and infection","volume":"28","author":"Lasa","year":"2005","journal-title":"An Sist Sanit Navar"},{"key":"2021033107574632100_CIT0018","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/S1473-3099(02)00228-1","article-title":"Watnick \u2013 elucidating the role of biofilms. Interview by Pam Das","volume":"2","author":"Watnick","year":"2002","journal-title":"Lancet Infect Dis"},{"key":"2021033107574632100_CIT0019","doi-asserted-by":"crossref","first-page":"9926","DOI":"10.1073\/pnas.1307697110","article-title":"Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples","volume":"110","author":"Bari","year":"2013","journal-title":"Proc. Nat. Acad. Sci. U.S.A"},{"key":"2021033107574632100_CIT0020","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1073\/pnas.0913404107","article-title":"Quorum-regulated biofilms enhance the development of conditionally viable, environmental Vibrio cholerae","volume":"107","author":"Kamruzzaman","year":"2010","journal-title":"Proc. Nat. Acad. Sci. U.S.A"},{"key":"2021033107574632100_CIT0021","doi-asserted-by":"crossref","first-page":"5266","DOI":"10.1128\/IAI.00578-08","article-title":"Effect of phage on the infectivity of Vibrio cholerae and emergence of genetic variants","volume":"76","author":"Zahid","year":"2008","journal-title":"Infect Immun"},{"key":"2021033107574632100_CIT0022","doi-asserted-by":"crossref","first-page":"6350","DOI":"10.1073\/pnas.0601277103","article-title":"Transmissibility of cholera: in vivo-formed biofilms and their relationship to infectivity and persistence in the environment","volume":"103","author":"Faruque","year":"2006","journal-title":"Proc. Nat. Acad. Sci. U.S.A"},{"key":"2021033107574632100_CIT0023","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1099\/jmm.0.013599-0","article-title":"Vibrio cholerae O1 Ogawa detoxified lipopolysaccharide structures as inducers of cytokines and oxidative species in macrophages","volume":"59","author":"Paulovicova","year":"2010","journal-title":"J. Med. Microbiol"},{"key":"2021033107574632100_CIT0024","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/nature09973","article-title":"TLR signalling augments macrophage bactericidal activity through mitochondrial ROS","volume":"472","author":"West","year":"2011","journal-title":"Nature"},{"key":"2021033107574632100_CIT0025","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.ejphar.2011.02.022","article-title":"Antioxidant and signal modulation properties of plant polyphenols in controlling vascular inflammation","volume":"658","author":"Kostyuk","year":"2011","journal-title":"Eur. J. Pharmacol"},{"key":"2021033107574632100_CIT0026","doi-asserted-by":"crossref","first-page":"9280","DOI":"10.1073\/pnas.0601754103","article-title":"A Vibrio cholerae protease needed for killing of Caenorhabditis elegans has a role in protection from natural predator grazing","volume":"103","author":"Vaitkevicius","year":"2006","journal-title":"Proc. Nat. Acad. Sci. U.S.A"},{"key":"2021033107574632100_CIT0027","doi-asserted-by":"crossref","first-page":"e11558","DOI":"10.1371\/journal.pone.0011558","article-title":"Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans","volume":"5","author":"Cinar","year":"2010","journal-title":"PLoS ONE"},{"key":"2021033107574632100_CIT0028","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1574-6968.2004.tb09643.x","article-title":"What drives bacteria to produce a biofilm?","volume":"236","author":"Jefferson","year":"2004","journal-title":"FEMS Microbiol. Lett"},{"key":"2021033107574632100_CIT0029","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/S1534-5807(03)00295-8","article-title":"Quorum sensing-dependent biofilms enhance colonization in Vibrio cholerae","volume":"5","author":"Zhu","year":"2003","journal-title":"Dev. Cell"},{"key":"2021033107574632100_CIT0030","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s00284-011-0073-4","article-title":"Arctic Actinomycetes as potential inhibitors of Vibrio cholerae biofilm","volume":"64","author":"Augustine","year":"2012","journal-title":"Curr. Microbiol"},{"key":"2021033107574632100_CIT0031","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1128\/AAC.36.4.788","article-title":"Interaction of Vibrio cholerae cells with beta-lactam antibiotics: emergence of resistant cells at a high frequency","volume":"36","author":"Sengupta","year":"1992","journal-title":"Antimicrob. Agents Chemother"},{"key":"2021033107574632100_CIT0032","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1248\/bpb.27.1965","article-title":"Antimicrobial activity of 10 different plant polyphenols against bacteria causing food-borne disease","volume":"27","author":"Taguri","year":"2004","journal-title":"Biol. Pharm. Bull"},{"key":"2021033107574632100_CIT0033","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/S1995-7645(13)60038-3","article-title":"In vitro and in vivo bactericidal activity of Vitex negundo leaf extract against diverse multidrug resistant enteric bacterial pathogens","volume":"6","author":"Kamruzzaman","year":"2013","journal-title":"Asia\u2013Pac.Trop. Med"},{"key":"2021033107574632100_CIT0034","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1016\/S0960-9822(00)00833-2","article-title":"Caenorhabditis elegans is a model host for Salmonella typhimurium","volume":"10","author":"Labrousse","year":"2000","journal-title":"Curr. Biol"},{"key":"2021033107574632100_CIT0035","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S0966-842X(99)01691-1","article-title":"Caenorhabditis elegans for the study of host-pathogen interactions","volume":"8","author":"Kurz","year":"2000","journal-title":"Trends Microbiol"},{"key":"2021033107574632100_CIT0036","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S1369-5274(99)00047-8","article-title":"Caenorhabditis elegans: a model genetic host to study Pseudomonas aeruginosa pathogenesis","volume":"3","author":"Tan","year":"2000","journal-title":"Curr. Opin. Microbiol"},{"key":"2021033107574632100_CIT0037","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.jep.2011.06.039","article-title":"Synergistic effects of baicalein with ciprofloxacin against NorA over-expressed methicillin-resistant Staphylococcus aureus (MRSA) and inhibition of MRSA pyruvate kinase","volume":"137","author":"Chan","year":"2011","journal-title":"J. Ethnopharmacol"},{"key":"2021033107574632100_CIT0038","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1472-6882-10-33","article-title":"Newer insights into the mechanism of action of Psidium guajava L. leaves in infectious diarrhoea","volume":"10","author":"Birdi","year":"2010","journal-title":"BMC Complem. Altern. Med"}],"container-title":["Bioscience, Biotechnology, and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bbb\/article-pdf\/79\/3\/475\/36807452\/bbb0475.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/bbb\/article-pdf\/79\/3\/475\/36807452\/bbb0475.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T11:48:17Z","timestamp":1654602497000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bbb\/article\/79\/3\/475\/5939348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,4]]},"references-count":38,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2015,3,4]]},"published-print":{"date-parts":[[2015,3,4]]}},"URL":"https:\/\/doi.org\/10.1080\/09168451.2014.991685","relation":{},"ISSN":["0916-8451","1347-6947"],"issn-type":[{"value":"0916-8451","type":"print"},{"value":"1347-6947","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2015,3,4]]},"published":{"date-parts":[[2015,3,4]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=tbbb20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=tbbb20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"}]}}