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AITC-induced stomatal closure accompanied by reactive oxygen species (ROS) and NO production, cytosolic alkalization and glutathione (GSH) depletion in V. faba. GSH monoethyl ester induced stomatal reopening and suppressed AITC-induced GSH depletion in guard cells. Exogenous catalase and a peroxidase inhibitor, salicylhydroxamic acid, inhibited AITC-induced stomatal closure, unlike an NAD(P)H oxidase inhibitor, diphenylene iodonium chloride. The peroxidase inhibitor also abolished the AITC-induced ROS production, NO production, and cytosolic alkalization. AITC-induced stomatal closure was suppressed by an NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, and an agent to acidify cytosol, butyrate. These results indicate that AITC-induced stomatal closure in V. faba as well as in A. thaliana and suggest that AITC signaling in guard cells is conserved in both plants.<\/jats:p>","DOI":"10.1080\/09168451.2015.1045827","type":"journal-article","created":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T12:02:06Z","timestamp":1433160126000},"page":"1737-1742","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":29,"title":["Allyl isothiocyanate induces stomatal closure in <i>Vicia faba<\/i>"],"prefix":"10.1080","volume":"79","author":[{"given":"Muhammad Abdus","family":"Sobahan","sequence":"first","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasima","family":"Akter","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Okuma","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misugi","family":"Uraji","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxiu","family":"Ye","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izumi C","family":"Mori","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimasa","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Murata","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2015,10,3]]},"reference":[{"key":"2021033114075223600_CIT0001","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/S1360-1385(02)02273-2","article-title":"Glucosinolate research in the Arabidopsis era","volume":"7","author":"Wittstock","year":"2002","journal-title":"Trends Plant Sci"},{"key":"2021033114075223600_CIT0002","doi-asserted-by":"publisher","first-page":"1900","DOI":"10.1111\/j.1365-3040.2011.02385.x","article-title":"Allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis","volume":"34","author":"Khokon","year":"2011","journal-title":"Plant Cell Environ"},{"key":"2021033114075223600_CIT0003","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1038\/35021067","article-title":"Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells","volume":"406","author":"Pei","year":"2000","journal-title":"Nature"},{"key":"2021033114075223600_CIT0004","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1111\/tpj.2006.45.issue-1","article-title":"ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis","volume":"45","author":"Bright","year":"2006","journal-title":"Plant J"},{"key":"2021033114075223600_CIT0005","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.1104\/pp.103.032250","article-title":"Cytoplasmic alkalization precedes reactive oxygen species production during methyl jasmonate- and abscisic acid-induced stomatal closure","volume":"134","author":"Suhita","year":"2004","journal-title":"Plant Physiol"},{"key":"2021033114075223600_CIT0006","doi-asserted-by":"publisher","first-page":"1398","DOI":"10.1104\/pp.106.091298","article-title":"The coronatine-insensitive 1 mutation reveals the hormonal signaling interaction between abscisic acid and methyl jasmonate in Arabidopsis guard cells. 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