{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T21:13:01Z","timestamp":1718226781493},"reference-count":45,"publisher":"Informa UK Limited","issue":"11","license":[{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Higher Education Research Promotion and National Research University Project of Thailand","award":["NRU541007; FC1.1.7 PhD"],"award-info":[{"award-number":["NRU541007; FC1.1.7 PhD"]}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,11,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This work revealed peanut seed prolamins likely displaying a defensive role besides the known nitrogen storage. Drought stress and proteomic approaches were used in varieties of peanuts to explore the prolamin member in association with a test against Aspergillus flavus spore germination. The stress effect was showed by aerial biomass, leaf content of malondialdehyde, and seed contamination by A. flavus. Sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis profiles were not informative for the antifungal polypeptides. From two-dimensional gel electrophoresis, the suspected polypeptides were those with pI 5.45\u20135.75 and sizes of 22.0\u201330.5\u00a0kDa specifically in Spanish-type peanuts. Regarding to the drought effect in most of these peanuts, the spot peak volume analysis deduced three novel prolamin-related antifungal polypeptides at pI 5.75\u20135.8 with 30.5, 27.5\u201328.5, and 22.0\u201322.5\u00a0kDa, which was confirmed after isoelectric purification at pH 5.60. The data could not yet conclude their correlation with resistance to drought and to seed infection by A. flavus.<\/jats:p>","DOI":"10.1080\/09168451.2015.1056508","type":"journal-article","created":{"date-parts":[[2015,6,18]],"date-time":"2015-06-18T15:27:01Z","timestamp":1434641221000},"page":"1771-1778","update-policy":"http:\/\/dx.doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":1,"title":["Identification of peanut seed prolamins with an antifungal role by 2D-GE and drought treatment"],"prefix":"10.1080","volume":"79","author":[{"given":"Waraluk","family":"Senakoon","sequence":"first","affiliation":[{"name":"Faculty of Science, Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suporn","family":"Nuchadomrong","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin Y-Y","family":"Chiou","sequence":"additional","affiliation":[{"name":"Department of Food Science, College of Life Sciences, National Chiayi University, Chiayi, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gulsiri","family":"Senawong","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanun","family":"Jogloy","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Department of Plant Science and Agricultural Resources, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patcharin","family":"Songsri","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Department of Plant Science and Agricultural Resources, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aran","family":"Patanothai","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Department of Plant Science and Agricultural Resources, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2015,11,2]]},"reference":[{"key":"2021033107573796200_CIT0001","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1016\/j.biotechadv.2011.11.001","article-title":"Advances in Arachis genomics for peanut improvement","volume":"30","author":"Pandey","year":"2012","journal-title":"Biotechnol. 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