{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:19:07Z","timestamp":1773659947352,"version":"3.50.1"},"reference-count":27,"publisher":"Informa UK Limited","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24300258"],"award-info":[{"award-number":["24300258"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24300258"],"award-info":[{"award-number":["24300258"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>B-group vitamins are involved in the catabolism of 2-oxo acids. To identify the functional biomarkers of B-group vitamins, we developed a high-performance liquid chromatographic method for profiling 2-oxo acids in urine and applied this method to urine samples from rats deficient in vitamins B1 and B6 and pantothenic acid. 2-Oxo acids were reacted with 1,2-diamino-4,5-methylenebenzene to produce fluorescent derivatives, which were then separated using a TSKgel ODS-80Ts column with 30\u00a0mmol\/L of KH2PO4 (pH 3.0):acetonitrile (7:3) at a flow rate of 1.0\u00a0mL\/min. Vitamin B1 deficiency increased urinary levels of all 2-oxo acids, while vitamin B6 deficiency only increased levels of sum of 2-oxaloacetic acid and pyruvic acid, and pantothenic acid deficiency only increased levels of 2-oxoisovaleric acid. Profiles of 2-oxo acids in urine samples might be a non-invasive way of clarifying the functional biomarker of B-group vitamins.<\/jats:p>","DOI":"10.1080\/09168451.2015.1083395","type":"journal-article","created":{"date-parts":[[2015,9,7]],"date-time":"2015-09-07T10:36:39Z","timestamp":1441622199000},"page":"304-312","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":7,"title":["High-performance liquid chromatographic method for profiling 2-oxo acids in urine and its application in evaluating vitamin status in rats"],"prefix":"10.1080","volume":"80","author":[{"given":"Katsumi","family":"Shibata","sequence":"first","affiliation":[{"name":"Department of Nutrition, School of Human Cultures, University of Shiga Prefecture, Hikone, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chifumi","family":"Nakata","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Human Cultures, University of Shiga Prefecture, Hikone, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Fukuwatari","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Human Cultures, University of Shiga Prefecture, Hikone, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2016,2,1]]},"reference":[{"key":"2021033107580907800_CIT0001","doi-asserted-by":"publisher","first-page":"223","DOI":"10.3177\/jnsv.54.223","article-title":"Urinary water-soluble vitamins and their metabolite contents as nutritional markers for evaluating vitamin intakes in young Japanese women","volume":"54","author":"Fukuwatari","year":"2008","journal-title":"J. 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