{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:19:00Z","timestamp":1790745540771,"version":"4.1.0"},"reference-count":34,"publisher":"Informa UK Limited","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"JSPS KAKENHI","award":["15H04486"],"award-info":[{"award-number":["15H04486"]}]},{"name":"JSPS KAKENHI","award":["15K07396"],"award-info":[{"award-number":["15K07396"]}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In the vacuolar basic amino acid (VBA) transporter family of Saccharomyces cerevisiae, VBA4 encodes a vacuolar membrane protein with 14 putative transmembrane helices. Transport experiments with isolated vacuolar membrane vesicles and estimation of the amino acid contents in vacuoles showed that Vba4p is not likely involved in the transport of amino acids. We found that the vba4\u0394 cells, as well as vba1\u0394 and vba2\u0394 cells, showed increased susceptibility to several drugs, particularly to azoles. Although disruption of the VBA4 gene did not affect the salt tolerance of the cells, vacuolar fragmentation observed under high salt conditions was less prominent in vba4\u0394 cells than in wild type, vba1\u0394, and vba2\u0394 cells. Vba4p differs from Vba1p and Vba2p as a vacuolar transporter but is important for the drug resistance and vacuolar morphology of S. cerevisiae.<\/jats:p>","DOI":"10.1080\/09168451.2015.1083401","type":"journal-article","created":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T15:52:53Z","timestamp":1441122773000},"page":"279-287","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":12,"title":["Vba4p, a vacuolar membrane protein, is involved in the drug resistance and vacuolar morphology of <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1080","volume":"80","author":[{"given":"Miyuki","family":"Kawano-Kawada","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"},{"name":"Advanced Research Support Center (ADRES), Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pongsanat","family":"Pongcharoen","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rieko","family":"Kawahara","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayu","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Akiyama","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"},{"name":"Advanced Research Support Center (ADRES), Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Sekito","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimi","family":"Kakinuma","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, Matsuyama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"301","published-online":{"date-parts":[[2016,2,1]]},"reference":[{"key":"2021033107573227500_CIT0001","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1567-1364.2006.00058.x","article-title":"Evolution of gene families: the multidrug resistance transporter genes in five related yeast species","volume":"6","author":"Gbelska","year":"2006","journal-title":"FEMS Yeast Res"},{"key":"2021033107573227500_CIT0002","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0168-1656(02)00133-5","article-title":"The multidrug resistance transporters of the major facilitator superfamily, 6\u00a0years after disclosure of Saccharomyces cerevisiae genome sequence","volume":"98","author":"S\u00e1-Correia","year":"2002","journal-title":"J. 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