{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:22:52Z","timestamp":1761744172196},"reference-count":36,"publisher":"Informa UK Limited","issue":"3","license":[{"start":{"date-parts":[[2016,3,3]],"date-time":"2016-03-03T00:00:00Z","timestamp":1456963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Biomass Utilization Project of Ministry of Agriculture, Forestry, and Fisheries of Japan."}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,3,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We obtained a novel glucose de-repressed mutant of Trichoderma reesei using disparity mutagenesis. A plasmid containing DNA polymerase \u03b4 lacking proofreading activity, and AMAI, an autonomously replicating sequence was introduced into T. reesei ATCC66589. The rate of mutation evaluated with 5-fluoroorotic acid resistance was approximately 30-fold higher than that obtained by UV irradiation. The transformants harboring incompetent DNA polymerase \u03b4 were then selected on 2-deoxyglucose agar plates with hygromycin B. The pNP-lactoside hydrolyzing activities of mutants were 2 to 5-fold higher than the parent in liquid medium containing glucose. Notably, the amino acid sequence of cre1, a key gene involved in glucose repression, was identical in the mutant and parent strains, and further, the cre1 expression levels was not abolished in the mutant. Taken together, these results demonstrate that the strains of T. reesei generated by disparity mutagenesis are glucose de-repressed variants that contain mutations in yet-unidentified factors other than cre1.<\/jats:p>","DOI":"10.1080\/09168451.2015.1104236","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T23:58:49Z","timestamp":1446767929000},"page":"486-492","update-policy":"http:\/\/dx.doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":9,"title":["Generation of a glucose de-repressed mutant of <i>Trichoderma reesei<\/i> using disparity mutagenesis"],"prefix":"10.1080","volume":"80","author":[{"given":"Hidekazu","family":"Iwakuma","sequence":"first","affiliation":[{"name":"Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, Fujisawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Koyama","sequence":"additional","affiliation":[{"name":"Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, Fujisawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Miyachi","sequence":"additional","affiliation":[{"name":"Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, Fujisawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Nasukawa","sequence":"additional","affiliation":[{"name":"Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, Fujisawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, Fujisawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuntaro","family":"Yano","sequence":"additional","affiliation":[{"name":"Neo-Morgan Laboratory Incorporated, Kawasaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Ogihara","sequence":"additional","affiliation":[{"name":"Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, Fujisawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Kasumi","sequence":"additional","affiliation":[{"name":"Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, Fujisawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2016,3,3]]},"reference":[{"key":"2021033114371625900_CIT0001","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1126\/science.1121416","article-title":"Ethanol can contribute to energy and environmental goals","volume":"311","author":"Farrell","year":"2006","journal-title":"Science"},{"key":"2021033114371625900_CIT0002","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/989572","article-title":"Bioconversion of sugarcane biomass into ethnol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzyme saccharification and ethanol fermentation","author":"Caniliha","year":"2012","journal-title":"J. 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