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Here, we report that vicenistatin, a natural product, enhanced homotypic fusion of early endosomes and induced the formation of large vacuole-like structures in mammalian cells. Unlike YM201636, another early endosome vacuolating compound, vicenistatin did not inhibit PIKfyve activity in vitro but activated Rab5-PAS pathway in cells. Furthermore, vicenistatin increased the membrane surface fluidity of cholesterol-containing liposomes in vitro, and cholesterol deprivation from the plasma membrane stimulated vicenistatin-induced vacuolation in cells. These results suggest that vicenistatin is a novel compound that induces the formation of vacuole-like structures by activating Rab5-PAS pathway and increasing membrane fluidity.<\/jats:p>","DOI":"10.1080\/09168451.2015.1132152","type":"journal-article","created":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T10:22:01Z","timestamp":1454322121000},"page":"902-910","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":14,"title":["Vicenistatin induces early endosome-derived vacuole formation in mammalian cells"],"prefix":"10.1080","volume":"80","author":[{"given":"Yuko","family":"Nishiyama","sequence":"first","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Ohmichi","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Kazami","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Group, RIKEN CSRS, Wako, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousuke","family":"Mano","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Nagumo","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumitaka","family":"Kudo","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sosaku","family":"Ichikawa","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiharu","family":"Iwabuchi","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Kanoh","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Eguchi","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Osada","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Group, RIKEN CSRS, Wako, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeo","family":"Usui","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"301","published-online":{"date-parts":[[2016,5,3]]},"reference":[{"key":"2021033109495229600_CIT0001","first-page":"331","article-title":"Rab5 regulates the dynamics of early endosome fusion","volume":"20","author":"Barbieri","year":"1996","journal-title":"Biocell"},{"key":"2021033109495229600_CIT0002","doi-asserted-by":"crossref","first-page":"24048","DOI":"10.1074\/jbc.271.39.24048","article-title":"Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger","volume":"271","author":"Stenmark","year":"1996","journal-title":"J. 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