{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:13:16Z","timestamp":1790370796429,"version":"4.1.0"},"reference-count":27,"publisher":"Informa UK Limited","issue":"5","license":[{"start":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T00:00:00Z","timestamp":1462233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,5,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Fluoroacetamide (Mw\u00a0=\u00a077.06) is a lethal rodenticide to humans and animals which is still frequently abused in food storage somewhere in China. The production of antibodies for fluoroacetamide is difficult due to its high toxicity to animals, which limits the application of immunoassay method in poison detection. In this work, aptamers targeting N-fluoroacetyl glycine as an analog of fluoroacetamide were selected by a specific systematic evolution of ligands by exponential enrichment (SELEX) strategy. The binding ability of the selected aptamers to fluoroacetamide was identified using surface plasmon resonance (SPR)-based assay. The estimated KD values in the low micromolar range showed a good affinity of these aptamers to the target. Our work verified that the SELEX strategy has the potential for developing aptamers targeted to small molecular toxicants and aptamers can be employed as new recognition elements instead of antibodies for poison detection.<\/jats:p>","DOI":"10.1080\/09168451.2015.1136876","type":"journal-article","created":{"date-parts":[[2016,2,14]],"date-time":"2016-02-14T04:35:00Z","timestamp":1455424500000},"page":"823-832","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":28,"title":["<i>In vitro<\/i> selection of DNA aptamers binding pesticide fluoroacetamide"],"prefix":"10.1080","volume":"80","author":[{"given":"Fangqi","family":"Cao","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Research Institute of Criminal Science and Technology, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Lu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai, China"},{"name":"State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Hu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libo","family":"Zeng","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liankang","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiqi","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"301","published-online":{"date-parts":[[2016,5,3]]},"reference":[{"key":"2021033109500669500_CIT0001","first-page":"256","volume-title":"Forensic toxicology handbook","author":"Shen","year":"2012"},{"key":"2021033109500669500_CIT0002","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.jchromb.2008.10.027","article-title":"Simultaneous determination of two acute poisoning rodenticides tetramine and fluoroacetamide with a coupled column in poisoning cases","volume":"876","author":"Xu","year":"2008","journal-title":"J. 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