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The HMW adiponectin oligomers possess the most potent insulin-sensitizing activity. WSF-P-1(N-methyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4\u03b1-methanonaphthalen-7-amine) is derived from natural sesquiterpene longifolene by chemical modifications. We found that WSF-P-1 activates AMPK in both 3T3-L1 adipocytes and 293T cells in this study. Activation of AMPK by WSF-P-1 promotes the assembly of HMW adiponectin and increases the HMW\/total ratio of adiponectin in 3T3-L1 adipocytes. We demonstrated that the Ca2+-dependent CaMKK signaling pathway is involved in WSF-P-1-induced AMPK activation and adiponectin multimerization. WSF-P-1 also activates GLUT1-mediated glucose uptake in 3T3-L1 adipocytes, making it a potential drug candidate for the treatment of type 2 diabetes, obesity, and other obesity-related metabolic diseases.<\/jats:p>","DOI":"10.1080\/09168451.2017.1336923","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T15:17:58Z","timestamp":1497367078000},"page":"1529-1535","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":6,"title":["WSF-P-1, a novel AMPK activator, promotes adiponectin multimerization in 3T3-L1 adipocytes"],"prefix":"10.1080","volume":"81","author":[{"given":"Yao","family":"Wang","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yudian","family":"Zhang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of 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