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The injection of a solution of megalo-type isomaltosaccharides (1\u20134%(w\/v), average DP\u00a0=\u00a012.6), but not oligo-type isomaltosaccharides (average DP\u00a0=\u00a03.3), into the intestinal lumen dose-dependently reduced the transport rates of tight junction permeable markers in a ligated loop of the anesthetized rat jejunum. Application of the megalosaccharide also suppressed the transport of tight junction markers and enhanced transepithelial electrical resistance (TEER) in Caco-2 cell monolayers. Cholesterol sequestration by methyl-\u03b2-cyclodextrin in the Caco-2 monolayers abolished the effect of megalosaccharide. Treatment with anti-caveolin-1 and a caveolae inhibitor, but not clathrin-dependent endocytosis and macropinocytosis inhibitors, suppressed the increase in TEER. These results indicate that isomaltosaccharides promote the barrier function of tight junctions in the intestinal epithelium in a chain-length dependent manner and that caveolae play a role in the effect.<\/jats:p>","DOI":"10.1080\/09168451.2017.1398065","type":"journal-article","created":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T08:36:09Z","timestamp":1511771769000},"page":"629-635","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":10,"title":["Enzymatically synthesized megalo-type isomaltosaccharides enhance the barrier function of the tight junction in the intestinal epithelium"],"prefix":"10.1080","volume":"82","author":[{"given":"Hiroshi","family":"Hara","sequence":"first","affiliation":[{"name":"Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Kume","sequence":"additional","affiliation":[{"name":"Research 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