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These compounds were designed and synthesized based on the structural attributes of a \u03b2-phenyl-\u03b1,\u03b2-unsaturated carbonyl scaffold template. Among these compounds, (Z)-5-(3-hydroxy-4-methoxybenzylidene)-2-iminothiazolidin-4-one (1e, MHY773) exhibited the greatest tyrosinase inhibition (IC50 = 2.87 \u03bcM and 8.06 \u03bcM for monophenolase and diphenolase), and outperformed the positive control, kojic acid (IC50 = 15.59 and 31.61 \u03bcM). The kinetic and docking studies demonstrated that MHY773 interacted with active site of tyrosinase. Moreover, a melanin quantification assay demonstrated that MHY773 attenuates \u03b1-melanocyte-stimulating hormone (\u03b1-MSH) and 3-isobutyl-1-methylxanthine (IBMX)-induced melanin contents in B16F10 melanoma cells. Taken together, these data suggest that MHY773 suppressed the melanin production via the inhibition of tyrosinase activity. MHY773 is a promising for the development of effective pharmacological and cosmetic agents for skin-whitening.<\/jats:p>","DOI":"10.1080\/09168451.2018.1445518","type":"journal-article","created":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T12:33:42Z","timestamp":1520598822000},"page":"759-767","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":23,"title":["A novel synthetic compound, (<i>Z<\/i>)-5-(3-hydroxy-4-methoxybenzylidene)-2-iminothiazolidin-4-one (MHY773) inhibits mushroom tyrosinase"],"prefix":"10.1080","volume":"82","author":[{"given":"Hee Jin","family":"Jung","sequence":"first","affiliation":[{"name":"Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Jung","family":"Lee","sequence":"additional","affiliation":[{"name":"Molecular Inflammation Research 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Pharmacy, Inje University, Gimhae, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Suk","family":"Jung","sequence":"additional","affiliation":[{"name":"Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, Republic of Korea"},{"name":"College of Pharmacy, Pusan National University, Busan, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyung Ryong","family":"Moon","sequence":"additional","affiliation":[{"name":"Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan, Republic of Korea"},{"name":"College of Pharmacy, Pusan National University, Busan, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hae Young","family":"Chung","sequence":"additional","affiliation":[{"name":"Molecular Inflammation Research Center for Aging Intervention (MRCA), 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