{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T01:02:49Z","timestamp":1793754169933,"version":"4.6.1"},"reference-count":44,"publisher":"Informa UK Limited","issue":"1","license":[{"start":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T00:00:00Z","timestamp":1546387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100002573","name":"Yonsei University","doi-asserted-by":"publisher","award":["Yonsei University Research Fund of 2018"],"award-info":[{"award-number":["Yonsei University Research Fund of 2018"]}],"id":[{"id":"10.13039\/501100002573","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002573","name":"Yonsei University","doi-asserted-by":"publisher","award":["Yonsei University Research Fund of 2018"],"award-info":[{"award-number":["Yonsei University Research Fund of 2018"]}],"id":[{"id":"10.13039\/501100002573","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,1,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Inflammation induced by Helicobacter pylori infection related to gastric carcinogenesis. In this study, we have investigated the anti-inflammatory effect and its mechanism of kaempferol in the inflammatory response caused by H. pylori infection in vitro. We found that kaempferol reduced the expression of pro-inflammatory cytokines (TNF-\u03b1, IL-1\u03b2, and IL-8) and production of IL-8 in AGS cells. In addition, kaempferol suppressed translocation of cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA) of H. pylori to AGS cells. It was due to decreased transcription of type IV secretion system (T4SS) components involved in CagA injection and secretion system subunit protein A (SecA) of type V secretion system (T5SS) involved in VacA secretion by kaempferol. In conclusion, kaempferol shows the anti-inflammatory effect by suppressing the translocation of CagA and VacA proteins and leading to the down-regulation of pro-inflammatory cytokines.<\/jats:p>\n                  <jats:p>Abbreviations: CagA: cytotoxin-associated gene A; VacA: vacuolating cytotoxin A; T4SS: type IV secretion systems; SecA: secretion system subunit protein A; T5SS: type V secretion system;<\/jats:p>","DOI":"10.1080\/09168451.2018.1528140","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T03:34:38Z","timestamp":1538710478000},"page":"166-173","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":89,"title":["Anti-inflammatory effects of Kaempferol on\n                    <i>Helicobacter pylori<\/i>\n                    -induced inflammation"],"prefix":"10.1080","volume":"83","author":[{"given":"Min Ji","family":"Yeon","sequence":"first","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Forensic DNA Division, National Forensic Service, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Yeong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Jun","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Jin","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheol","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Semyung University, Jecheon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa-Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, Semyung University, Jecheon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Bae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"301","published-online":{"date-parts":[[2019,1,2]]},"reference":[{"key":"2021033109271990300_CIT0001","volume-title":"Helicobacter pylori: physiology and genetics","author":"O\u2019Rourke"},{"key":"2021033109271990300_CIT0002","volume-title":"Helicobacter pylori: physiology and Genetics","author":"Krishnamurthy"},{"key":"2021033109271990300_CIT0003","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1186\/1471-2180-13-103","article-title":"Antibacterial activities of almond skins on cagA-positive and-negative clinical isolates of Helicobacter pylori","volume":"13","author":"Bisignano","year":"2013","journal-title":"BMC Microbiol"},{"issue":"5","key":"2021033109271990300_CIT0004","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12399","article-title":"Review: prevalence and dynamics of Helicobacter pylori infection during childhood","volume":"22","author":"Zabala Torrres","year":"2017","journal-title":"Helicobacter"},{"issue":"4","key":"2021033109271990300_CIT0005","doi-asserted-by":"crossref","first-page":"e1003251","DOI":"10.1371\/journal.ppat.1003251","article-title":"Caveolin-1 protects B6129 mice against Helicobacter pylori gastritis","volume":"9","author":"Hitkova","year":"2013","journal-title":"PLoS Pathog"},{"key":"2021033109271990300_CIT0006","doi-asserted-by":"crossref","first-page":"935410","DOI":"10.5402\/2012\/935410","article-title":"Eradication treatment of Helicobacter pylori infection: its importance and possible relationship in preventing the development of gastric cancer","volume":"2012","author":"Roesler","year":"2012","journal-title":"ISRN Gastroenterol"},{"issue":"3","key":"2021033109271990300_CIT0007","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/jcb.24389","article-title":"Role of the Helicobacter pylori-induced inflammatory response in the development of gastric cancer","volume":"114","author":"Lamb","year":"2013","journal-title":"J Cell Biochem"},{"issue":"2","key":"2021033109271990300_CIT0008","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.canlet.2013.08.016","article-title":"Helicobacter pylori-induced gastric inflammation and gastric cancer","volume":"345","author":"Wang","year":"2014","journal-title":"Cancer Lett"},{"issue":"36","key":"2021033109271990300_CIT0009","doi-asserted-by":"crossref","first-page":"12767","DOI":"10.3748\/wjg.v20.i36.12767","article-title":"Effect of Helicobacter pylori on gastric epithelial cells","volume":"20","author":"Alzahrani","year":"2014","journal-title":"World J Gastroenterol"},{"issue":"8","key":"2021033109271990300_CIT0010","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1111\/j.1742-4658.2011.08037.x","article-title":"Architecture of the Helicobacter pylori Cag-type IV secretion system","volume":"278","author":"Terradot","year":"2011","journal-title":"FEBS J"},{"issue":"1\u20132","key":"2021033109271990300_CIT0011","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1080\/09687860500063316","article-title":"Structural and dynamic properties of bacterial type IV secretion systems (review)","volume":"22","author":"Christie","year":"2005","journal-title":"Mol Membr Biol"},{"issue":"1","key":"2021033109271990300_CIT0012","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1172\/JCI11830","article-title":"NF-kappaB: a key role in inflammatory diseases","volume":"107","author":"Tak","year":"2001","journal-title":"J Clin Invest"},{"issue":"7","key":"2021033109271990300_CIT0013","doi-asserted-by":"crossref","first-page":"742","DOI":"10.7150\/ijms.7167","article-title":"Cyanidin 3-O-glucoside reduces Helicobacter pylori VacA-induced cell death of gastric KATO III cells through inhibition of the SecA pathway","volume":"11","author":"Kim","year":"2014","journal-title":"Int J Med Sci"},{"issue":"3","key":"2021033109271990300_CIT0014","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nrmicro2733","article-title":"From self sufficiency to dependence: mechanisms and factors important for autotransporter biogenesis","volume":"10","author":"Leyton","year":"2012","journal-title":"Nat Rev Microbiol"},{"issue":"1","key":"2021033109271990300_CIT0015","doi-asserted-by":"crossref","first-page":"59","DOI":"10.15171\/bi.2017.08","article-title":"Pleiotropic cytotoxicity of VacA toxin in host cells and its impact on immunotherapy","volume":"7","author":"Fahimi","year":"2017","journal-title":"Bioimpacts"},{"key":"2021033109271990300_CIT0016","doi-asserted-by":"crossref","first-page":"92","DOI":"10.3389\/fcimb.2012.00092","article-title":"Vacuolating cytotoxin A (VacA), a key toxin for Helicobacter pylori pathogenesis","volume":"2","author":"Palframan","year":"2012","journal-title":"Front Cell Infect Microbiol"},{"issue":"4","key":"2021033109271990300_CIT0017","doi-asserted-by":"crossref","first-page":"298","DOI":"10.2174\/138955711795305335","article-title":"A review on the dietary flavonoid kaempferol","volume":"11","author":"Calderon-Montano","year":"2011","journal-title":"Mini Rev Med Chem"},{"key":"2021033109271990300_CIT0018","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.phrs.2015.05.002","article-title":"Kaempferol and inflammation: from chemistry to medicine","volume":"99","author":"Devi","year":"2015","journal-title":"Pharmacol Res"},{"issue":"3","key":"2021033109271990300_CIT0019","first-page":"390","article-title":"Effect of apigenin, kaempferol and resveratrol on the expression of interleukin-1beta and tumor necrosis factor-alpha genes in J774.2 macrophages","volume":"57","author":"Kowalski","year":"2005","journal-title":"Pharmacol Rep"},{"issue":"5","key":"2021033109271990300_CIT0020","doi-asserted-by":"crossref","first-page":"265","DOI":"10.5483\/BMBRep.2009.42.5.265","article-title":"Inhibitory effects of flavonoids on TNF-alpha-induced IL-8 gene expression in HEK 293 cells","volume":"42","author":"Lee","year":"2009","journal-title":"BMB Rep"},{"key":"2021033109271990300_CIT0021","doi-asserted-by":"crossref","first-page":"45673","DOI":"10.1155\/2007\/45673","article-title":"Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-kappaB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages","volume":"2007","author":"Hamalainen","year":"2007","journal-title":"Mediators Inflamm"},{"issue":"3","key":"2021033109271990300_CIT0022","first-page":"683","article-title":"Flavonoids inhibit tumor necrosis factor-alpha-induced up-regulation of intercellular adhesion molecule-1 (ICAM-1) in respiratory epithelial cells through activator protein-1 and nuclear factor-kappaB: structure-activity relationships","volume":"66","author":"Chen","year":"2004","journal-title":"Mol Pharmacol"},{"issue":"1","key":"2021033109271990300_CIT0023","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s005350170147","article-title":"Antibacterial action of tryptanthrin and kaempferol, isolated from the indigo plant (Polygonum tinctorium Lour.), against Helicobacter pylori-infected Mongolian gerbils","volume":"36","author":"Kataoka","year":"2001","journal-title":"J Gastroenterol"},{"issue":"5","key":"2021033109271990300_CIT0024","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1021\/np020472m","article-title":"Sesquiterpene lactones from Anthemis altissima and their anti-Helicobacter pylori activity","volume":"66","author":"Konstantinopoulou","year":"2003","journal-title":"J Nat Prod"},{"issue":"1","key":"2021033109271990300_CIT0025","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ijantimicag.2009.01.010","article-title":"Antimicrobial activity against Helicobacter pylori strains and antioxidant properties of blackberry leaves (Rubus ulmifolius) and isolated compounds","volume":"34","author":"Martini","year":"2009","journal-title":"Int J Antimicrob Agents"},{"issue":"2","key":"2021033109271990300_CIT0026","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.jep.2013.09.008","article-title":"Evaluation of the toxicity and antimicrobial activity of hydroethanolic extract of Arrabidaea chica (Humb. & Bonpl.) B. Verl","volume":"150","author":"Mafioleti","year":"2013","journal-title":"J Ethnopharmacol"},{"issue":"10","key":"2021033109271990300_CIT0027","doi-asserted-by":"crossref","first-page":"838","DOI":"10.7150\/ijms.5094","article-title":"Inhibitory effects of anthocyanins on secretion of Helicobacter pylori CagA and VacA toxins","volume":"9","author":"Kim","year":"2012","journal-title":"Int J Med Sci"},{"key":"2021033109271990300_CIT0028","first-page":"S33","article-title":"Mucosal interleukin-8 and Helicobacter pylori-associated gastroduodenal disease","volume":"6 Suppl 1","author":"Crabtree","year":"1994","journal-title":"Eur J Gastroenterol Hepatol"},{"issue":"3","key":"2021033109271990300_CIT0029","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.4049\/jimmunol.155.3.1428","article-title":"IL-8 induces neutrophil chemotaxis predominantly via type I IL-8 receptors","volume":"155","author":"Hammond","year":"1995","journal-title":"J Immunol"},{"issue":"1","key":"2021033109271990300_CIT0030","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s10753-014-0011-2","article-title":"Protective effect of kaempferol on LPS plus ATP-induced inflammatory response in cardiac fibroblasts","volume":"38","author":"Tang","year":"2015","journal-title":"Inflammation"},{"key":"2021033109271990300_CIT0031","doi-asserted-by":"crossref","first-page":"485201","DOI":"10.1155\/2013\/485201","article-title":"Dietary flavonoids as therapeutics for preterm birth: luteolin and kaempferol suppress inflammation in human gestational tissues in vitro","volume":"2013","author":"Wall","year":"2013","journal-title":"Oxid Med Cell Longev"},{"issue":"4","key":"2021033109271990300_CIT0032","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s10522-007-9083-9","article-title":"Down-regulation of iNOS and TNF-alpha expression by kaempferol via NF-kappaB inactivation in aged rat gingival tissues","volume":"8","author":"Kim","year":"2007","journal-title":"Biogerontology"},{"issue":"7","key":"2021033109271990300_CIT0033","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1440-1746.1997.tb00469.x","article-title":"Helicobacter pylori induces an array of pro-inflammatory cytokines in human gastric epithelial cells: quantification of mRNA for interleukin-8, \u22121 alpha\/beta, granulocyte-macrophage colony-stimulating factor, monocyte chemoattractant protein-1 and tumour necrosis factor-alpha","volume":"12","author":"Jung","year":"1997","journal-title":"J Gastroenterol Hepatol"},{"issue":"11","key":"2021033109271990300_CIT0034","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1038\/embor.2009.210","article-title":"Helicobacter pylori CagA activates NF-kappaB by targeting TAK1 for TRAF6-mediated Lys 63 ubiquitination","volume":"10","author":"Lamb","year":"2009","journal-title":"EMBO Rep"},{"issue":"26","key":"2021033109271990300_CIT0035","doi-asserted-by":"crossref","first-page":"9300","DOI":"10.1073\/pnas.0409873102","article-title":"NF-kappaB activation and potentiation of proinflammatory responses by the Helicobacter pylori CagA protein","volume":"102","author":"Brandt","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"2021033109271990300_CIT0036","doi-asserted-by":"crossref","first-page":"e56291","DOI":"10.1371\/journal.pone.0056291","article-title":"Presence of terminal EPIYA phosphorylation motifs in Helicobacter pylori CagA contributes to IL-8 secretion, irrespective of the number of repeats","volume":"8","author":"Papadakos","year":"2013","journal-title":"PLoS One"},{"issue":"7","key":"2021033109271990300_CIT0037","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1128\/JB.186.7.1919-1926.2004","article-title":"Unveiling molecular scaffolds of the type IV secretion system","volume":"186","author":"Yeo","year":"2004","journal-title":"J Bacteriol"},{"issue":"8","key":"2021033109271990300_CIT0038","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1111\/j.1742-4658.2011.08036.x","article-title":"Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus","volume":"278","author":"Fischer","year":"2011","journal-title":"FEBS J"},{"issue":"1","key":"2021033109271990300_CIT0039","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0924-8579(00)00122-9","article-title":"Quantitative RT-PCR analysis of multiple genes encoding putative metronidazole nitroreductases from Helicobacter pylori","volume":"15","author":"Kwon","year":"2000","journal-title":"Int J Antimicrob Agents"},{"issue":"7","key":"2021033109271990300_CIT0040","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1002\/mnfr.201000548","article-title":"Antiadhesion as a functional concept for prevention of pathogens: N-Phenylpropenoyl-L-amino acid amides as inhibitors of the Helicobacter pylori BabA outer membrane protein","volume":"55","author":"Niehues","year":"2011","journal-title":"Mol Nutr Food Res"},{"issue":"3","key":"2021033109271990300_CIT0041","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700586","article-title":"Regulatory effects of black rice extract on Helicobacter pylori infection-induced apoptosis","volume":"62","author":"Kim","year":"2018","journal-title":"Mol Nutr Food Res"},{"issue":"9","key":"2021033109271990300_CIT0042","doi-asserted-by":"crossref","first-page":"17288","DOI":"10.3390\/molecules200917288","article-title":"Effect of amaranthus on advanced glycation end-products induced cytotoxicity and proinflammatory cytokine gene expression in SH-SY5Y cells","volume":"20","author":"Amornrit","year":"2015","journal-title":"Molecules"},{"issue":"7","key":"2021033109271990300_CIT0043","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1046\/j.1365-2222.2001.01112.x","article-title":"Rhinovirus infection up-regulates eotaxin and eotaxin-2 expression in bronchial epithelial cells","volume":"31","author":"Papadopoulos","year":"2001","journal-title":"Clin Exp Allergy"},{"issue":"2","key":"2021033109271990300_CIT0044","first-page":"885","article-title":"Piperine treatment suppresses Helicobacter pylori toxin entry in to gastric epithelium and minimizes beta-catenin mediated oncogenesis and IL-8 secretion in vitro","volume":"8","author":"Tharmalingam","year":"2016","journal-title":"Am J Transl Res"}],"container-title":["Bioscience, Biotechnology, and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bbb\/article-pdf\/83\/1\/166\/36826220\/bbb0166.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/bbb\/article-pdf\/83\/1\/166\/36826220\/bbb0166.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T13:04:52Z","timestamp":1693919092000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bbb\/article\/83\/1\/166\/5955637"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,2]]},"references-count":44,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1,2]]},"published-print":{"date-parts":[[2019,1,2]]}},"URL":"https:\/\/doi.org\/10.1080\/09168451.2018.1528140","relation":{},"ISSN":["0916-8451","1347-6947"],"issn-type":[{"value":"0916-8451","type":"print"},{"value":"1347-6947","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,1,2]]},"published":{"date-parts":[[2019,1,2]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=tbbb20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=tbbb20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"},{"value":"2018-05-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}