{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:07:48Z","timestamp":1791846468084,"version":"4.3.4"},"reference-count":25,"publisher":"Informa UK Limited","issue":"7","license":[{"start":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T00:00:00Z","timestamp":1562112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"JSPS Grant-in-Aid for Scientific Research","award":["15H02442"],"award-info":[{"award-number":["15H02442"]}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,7,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) suppresses food intake after its activation by binding of its ligands, R-spondins. We investigated the mechanism of food intake suppression by R-spondin1 in a region-specific Lgr4 gene knockout (LGR4 cKO) mouse model, generated by deletion of the Lgr4 gene in arcuate nucleus (ARC) using Lgr4fx\/fx mice combined with infection of an AAV-Cre vector. After R-spondin1 administration, LGR4 cKO mice didn\u2019t exhibit a suppressed appetite, compared to that in control mice, which received a vehicle. In ARC of LGR4 cKO mice, Pomc mRNA expression was reduced, leading to suppressed food intake. On the other hand, neurons-specific LGR4 KO mice exhibited no differences in Pomc expression, and no structural differences were observed in the ARC of mutant mice. These results suggest that LGR4 is an essential part of the mechanism, inducing Pomc gene expression with R-spondin1 in ARC neurons in mice, thereby regulating feeding behavior.<\/jats:p>\n               <jats:p>Abbreviations: LGR4: Leucine-rich repeat-containing G-protein coupled receptor 4; RSPOs: roof plate-specific spondins; ARC: arcuate nucleus; AAV: adeno associated virus; POMC: pro-opiomelanocortin; CART: cocaine and amphetamine-regulated transcript; NPY: neuropeptide Y; AgRP: agouti-related peptide; Axin2: axis inhibition protein 2; Lef1: lymphoid enhancer binding factor 1; ccnd1: cyclin D1<\/jats:p>","DOI":"10.1080\/09168451.2019.1591266","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T22:40:02Z","timestamp":1553726402000},"page":"1336-1342","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":7,"title":["LGR4 is essential for R-spondin1-mediated suppression of food intake via pro-opiomelanocortin"],"prefix":"10.1080","volume":"83","author":[{"given":"Ayano","family":"Otsuka","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayana","family":"Jinguji","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Maejima","sequence":"additional","affiliation":[{"name":"Department of Bioregulation and Pharmacological Medicine, School of Medicine, Fukushima Medical University, Fukushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Kasahara","sequence":"additional","affiliation":[{"name":"Advanced Interdisciplinary Biomedical Engineering, Graduate School of 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