{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:02:17Z","timestamp":1777474937686,"version":"3.51.4"},"reference-count":26,"publisher":"Informa UK Limited","issue":"12","license":[{"start":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T00:00:00Z","timestamp":1575244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,12,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>A novel insecticidal peptide (LaIT3) was isolated from the Liocheles australasiae venom. The primary structure of LaIT3 was determined by a combination of Edman degradation and MS\/MS de novo sequencing analysis. Discrimination between Leu and Ile in MS\/MS analysis was achieved based on the difference in side chain fragmentation assisted by chemical derivatization. LaIT3 was determined to be an 84-residue peptide with three intrachain disulfide bonds. The sequence similarity search revealed that LaIT3 belongs to the scorpine-like peptides consisting of two structural domains: an N-terminal \u03b1-helical domain and a C-terminal cystine-stabilized domain. As observed for most of the scorpine-like peptides, LaIT3 showed significant antibacterial activity against Escherichia coli, which is likely to be caused by its membrane-disrupting property.<\/jats:p>","DOI":"10.1080\/09168451.2019.1654849","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T06:36:49Z","timestamp":1566801409000},"page":"2183-2189","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":13,"title":["Isolation and characterization of the insecticidal, two-domain toxin LaIT3 from the <i>Liocheles australasiae<\/i> scorpion venom"],"prefix":"10.1080","volume":"83","author":[{"given":"Hironori","family":"Juichi","sequence":"first","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Miyashita","sequence":"additional","affiliation":[{"name":"Graduate School of 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