{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:12:01Z","timestamp":1777673521140,"version":"3.51.4"},"reference-count":40,"publisher":"Informa UK Limited","issue":"1","license":[{"start":{"date-parts":[[2020,1,2]],"date-time":"2020-01-02T00:00:00Z","timestamp":1577923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100010256","name":"Science and Technology Project of the Changjiang River Administration of Navigational Affairs","doi-asserted-by":"publisher","award":["201810010"],"award-info":[{"award-number":["201810010"]}],"id":[{"id":"10.13039\/501100010256","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,1,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Plumbagin (PLB), an alkaloid obtained from the roots of the plants of Plumbago genus, is an inhibitor of NADPH oxidase 4 (NOX4). This study aimed to investigate the beneficial effect of PLB against oxygen-glucose deprivation\/reoxygenation (OGDR)-induced neuroinjury in human SH-SY5Y neuronal cultures. Our results showed that OGD\/R stimulated NOX4 protein expression and reactive oxygen species (ROS) production in SH-SY5Y cells, whereas increased 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA) production, resulting in the activation of the NLRP3 inflammasome. And PLB pretreatment reduced the ROS production by regulating the expression of NOX4 and downregulated NF-\u03baB signaling which was induced by OGDR. Furthermore, PLB inhibited OGDR induced NLRP3 inflammasome activation but not PARP1. Overall, PLB improved OGDR induced neuroinjury by inhibiting NOX4-derived ROS-activated NLRP3 inflammasome.<\/jats:p>","DOI":"10.1080\/09168451.2019.1664893","type":"journal-article","created":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T14:24:55Z","timestamp":1567779895000},"page":"134-142","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":24,"title":["Plumbagin attenuated oxygen-glucose deprivation\/reoxygenation-induced injury in human SH-SY5Y cells by inhibiting NOX4-derived ROS-activated NLRP3 inflammasome"],"prefix":"10.1080","volume":"84","author":[{"given":"Qianrui","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacy, General Hospital of the Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, General Hospital of the Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxia","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, General Hospital of the Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haitan","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, General Hospital of the Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Wuhan Fourth Hospital, Wuhan Puai Hospital, Tongji Medical College\uff0cHuazhong University of Science and Technology, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, General Hospital of the Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2020,1,2]]},"reference":[{"key":"2021033116172689700_CIT0001","doi-asserted-by":"crossref","first-page":"42","DOI":"10.2174\/18715273113126660189","article-title":"Necroptosis: who knew there were so many interesting ways to die?","volume":"13","author":"Fayaz","year":"2014","journal-title":"CNS Neurol Disord Drug Targets"},{"key":"2021033116172689700_CIT0002","doi-asserted-by":"crossref","first-page":"71","DOI":"10.14336\/AD.2016.0530","article-title":"Long non-coding RNA H19 induces cerebral ischemia reperfusion injury via activation of autophagy","volume":"8","author":"Wang","year":"2017","journal-title":"Aging Dis"},{"key":"2021033116172689700_CIT0003","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.2147\/IJN.S138178","article-title":"Kalopanacis cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen-glucose deprivation and reoxygenation","volume":"12","author":"Park","year":"2017","journal-title":"Int J Nanomedicine"},{"key":"2021033116172689700_CIT0004","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s12031-016-0712-0","article-title":"MicroRNA-25 negatively regulates cerebral ischemia\/reperfusioninjury-induced cell apoptosis through Fas\/FasL pathway","volume":"58","author":"Zhang","year":"2016","journal-title":"J Mol Neurosci"},{"key":"2021033116172689700_CIT0005","doi-asserted-by":"crossref","first-page":"30081","DOI":"10.3402\/fnr.v60.30081","article-title":"Dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation induced oxidative stress via reducing apoptosis","volume":"60","author":"Sun","year":"2016","journal-title":"Food Nutr Res"},{"key":"2021033116172689700_CIT0006","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1038\/srep00896","article-title":"Pharmacological inhibition of TLR4-NOX4 signal protects against neuronal death in transient focal ischemia","volume":"2","author":"Suzuki","year":"2012","journal-title":"Sci Rep"},{"issue":"Suppl 1","key":"2021033116172689700_CIT0007","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/jnc.13036","article-title":"Exosome-mediated inflammasome signaling after central nervous system injury","volume":"136","author":"de Rivero Vaccari","year":"2016","journal-title":"J Neurochem"},{"key":"2021033116172689700_CIT0008","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/9238290","article-title":"Recent advances of the NLRP3 inflammasome in central nervous system disorders","volume":"2016","author":"Zhou","year":"2016","journal-title":"J Immunol Res"},{"key":"2021033116172689700_CIT0009","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1038\/nature10759","article-title":"Inflammasomes in health and disease","volume":"481","author":"Strowig","year":"2012","journal-title":"Nature"},{"key":"2021033116172689700_CIT0010","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.jhep.2012.03.035","article-title":"Inflammasomes in liver diseases","volume":"57","author":"Szabo","year":"2012","journal-title":"J Hepatol"},{"key":"2021033116172689700_CIT0011","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1089\/ars.2015.6498","article-title":"Angiotensin-(1-7) improves liver fibrosis by regulating the NLRP3 inflammasome via redox balance modulation","volume":"24","author":"Cai","year":"2016","journal-title":"Antioxid Redox Signal"},{"key":"2021033116172689700_CIT0012","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/nature09663","article-title":"A role for mitochondria in NLRP3 inflammasome activation","volume":"469","author":"Zhou","year":"2011","journal-title":"Nature"},{"key":"2021033116172689700_CIT0013","first-page":"573","article-title":"The naphthoquinone plumbagin suppresses ADP-induced rat platelet aggregation through P2Y1-PLC signaling pathway","volume":"30","author":"Zhang","year":"2017","journal-title":"Pak J Pharm Sci"},{"key":"2021033116172689700_CIT0014","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1007\/s10753-013-9768-y","article-title":"Plumbagin, a vitamin K3 analogue, abrogates lipopolysaccharide-induced oxidative stress, in\ufb02ammation and endotoxic shock via NF-kappaB suppression","volume":"37","author":"Checker","year":"2014","journal-title":"Inflammation"},{"key":"2021033116172689700_CIT0015","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.canlet.2014.11.031","article-title":"Plumbagin induces apoptosis in lymphoma cells via oxidative stress mediated glutathionylation and inhibition of mitogen-activated protein kinase phosphatases (MKP1\/2)","volume":"357","author":"Checker","year":"2015","journal-title":"Cancer Lett"},{"key":"2021033116172689700_CIT0016","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/j.sjbs.2017.03.006","article-title":"Plumbagin inhibits neuronal apoptosis, intimal hyperplasia and also suppresses TNF-\u03b1\/NF-\u03baB pathway induced inflammation and matrix metalloproteinase-2\/9 expression in rat cerebral ischemia","volume":"25","author":"Chen","year":"2018","journal-title":"Saudi J Biol Sci"},{"key":"2021033116172689700_CIT0017","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1159\/000445592","article-title":"Exogenous HGF prevents cardiomyocytes from apoptosis after hypoxia via up-regulating cell autophagy","volume":"38","author":"Wang","year":"2016","journal-title":"Cell Physiol Biochem"},{"key":"2021033116172689700_CIT0018","doi-asserted-by":"crossref","first-page":"e17449","DOI":"10.1590\/s2175-97902018000417449","article-title":"Swertiamarin attenuates carbon tetrachloride (CCl4)-induced liver injury and in\ufb02ammation in rats by regulating the TLR4 signaling pathway","volume":"54","author":"Wu","year":"2018","journal-title":"Braz J Pharm Sci"},{"key":"2021033116172689700_CIT0019","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1007\/s11064-013-0968-5","article-title":"Oxygen glucose deprivation (OGD)\/re-oxygenation-induced in vitro neuronal cell death involves mitochondrial cyclophilin-D\/P53 signaling axis","volume":"38","author":"Zhao","year":"2013","journal-title":"Neurochem Res"},{"key":"2021033116172689700_CIT0020","doi-asserted-by":"crossref","first-page":"4162","DOI":"10.3892\/mmr.2016.5747","article-title":"Cerebral ischemic postconditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation cellular model","volume":"14","author":"Wang","year":"2016","journal-title":"Mol Med Rep"},{"key":"2021033116172689700_CIT0021","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3164\/jcbn.16-34","article-title":"Reactive oxygen species induce neurite degeneration before induction of cell death","volume":"59","author":"Fukui","year":"2016","journal-title":"J Clin Biochem Nutr"},{"key":"2021033116172689700_CIT0022","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1089\/ars.2010.3576","article-title":"Oxidative stress in ischemic brain damage: mechanisms of cell death and potential molecular targets for neuroprotection","volume":"14","author":"Chen","year":"2011","journal-title":"Antioxid Redox Signal"},{"key":"2021033116172689700_CIT0023","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1152\/physrev.00044.2005","article-title":"The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology","volume":"87","author":"Bedard","year":"2007","journal-title":"Physiol Rev"},{"key":"2021033116172689700_CIT0024","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0006-8993(03)03364-X","article-title":"Nadph oxidase immunoreactivity in the mouse brain","volume":"988","author":"Serrano","year":"2003","journal-title":"Brain Res"},{"key":"2021033116172689700_CIT0025","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.freeradbiomed.2017.01.034","article-title":"Combined NADPH and the NOX inhibitor apocynin provides greater anti-inflammatory and neuroprotective effects in a mouse model of stroke","volume":"104","author":"Qin","year":"2017","journal-title":"Free Radic Biol Med"},{"key":"2021033116172689700_CIT0026","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1089\/ars.2012.4797","article-title":"Neuroprotection after stroke by targeting nox4 as a source of oxidative stress","volume":"18","author":"Radermacher","year":"2013","journal-title":"Antioxid Redox Signal"},{"key":"2021033116172689700_CIT0027","doi-asserted-by":"crossref","first-page":"e1000479","DOI":"10.1371\/journal.pbio.1000479","article-title":"Post-stroke inhibition of induced nadph oxidase type 4 prevents oxidative stress and neurodegeneration","volume":"8","author":"Kleinschnitz","year":"2010","journal-title":"PLoS Biol"},{"key":"2021033116172689700_CIT0028","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3389\/fimmu.2012.00288","article-title":"NLRP3-inflammasome activating DAMPs stimulate an inflammatory response in glia in the absence of priming which contributes to brain inflammation after injury","volume":"3","author":"Savage","year":"2012","journal-title":"Front Immunol"},{"key":"2021033116172689700_CIT0029","doi-asserted-by":"crossref","first-page":"e0130624","DOI":"10.1371\/journal.pone.0130624","article-title":"NLRP3 inflammasome is expressed and functional in mouse brain microglia but not in astrocytes","volume":"10","author":"Gustin","year":"2015","journal-title":"PLoS One"},{"key":"2021033116172689700_CIT0030","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00011-016-0981-7","article-title":"NLRP3 inflammasome: a promising target in ischemic stroke","volume":"66","author":"Gao","year":"2017","journal-title":"Inflamm Res"},{"key":"2021033116172689700_CIT0031","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1007\/s11064-013-1115-z","article-title":"Expression of the NLRP3 inflammasome in cerebral cortex after traumatic brain injury in a rat model","volume":"38","author":"Liu","year":"2013","journal-title":"Neurochem Res"},{"key":"2021033116172689700_CIT0032","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/imr.12296","article-title":"Regulation of inflammasome activation","volume":"265","author":"Man","year":"2015","journal-title":"Immunol Rev"},{"key":"2021033116172689700_CIT0033","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.1002\/smll.201302825","article-title":"Polyhydroxylated metallofullerenols stimulate IL-1\u03b2 secretion of macrophage through TLRs\/MyD88\/NF-\u03baB pathway and NLRP\u2083 inflammasome activation","volume":"10","author":"Chen","year":"2014","journal-title":"Small"},{"key":"2021033116172689700_CIT0034","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1089\/ars.2014.5994","article-title":"Redox regulation of NLRP3 inflammasomes: ROS as trigger or effector?","volume":"22","author":"Abais","year":"2015","journal-title":"Antioxid Redox Signal"},{"key":"2021033116172689700_CIT0035","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.jep.2010.02.022","article-title":"Cinobufacini, an aqueous extract from Bufo bufo gargarizans Cantor, induces apoptosis through a mitochondria-mediated pathway in human hepatocellular carcinoma cells","volume":"128","author":"Qi","year":"2010","journal-title":"J Ethnopharmacol"},{"key":"2021033116172689700_CIT0036","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0005-2728(98)00110-8","article-title":"Mitochondria as regulators of apoptosis: doubt no more","volume":"1366","author":"Susin","year":"1998","journal-title":"Biochim Biophys Acta"},{"key":"2021033116172689700_CIT0037","doi-asserted-by":"crossref","first-page":"4511","DOI":"10.18632\/oncotarget.23391","article-title":"AMPK activation by Tanshinone IIA protects neuronal cells from oxygen-glucose deprivation","volume":"9","author":"Weng","year":"2017","journal-title":"Oncotarget"},{"key":"2021033116172689700_CIT0038","doi-asserted-by":"crossref","first-page":"e60065","DOI":"10.1371\/journal.pone.0060065","article-title":"Resveratrol induces premature senescence in lung cancer cells via ROS-mediated DNA damage","volume":"8","author":"Luo","year":"2013","journal-title":"PLoS One"},{"key":"2021033116172689700_CIT0039","doi-asserted-by":"crossref","first-page":"2","DOI":"10.2353\/ajpath.2008.080019","article-title":"Role of the peroxynitrite-poly (ADP-ribose) polymerase pathway in human disease","volume":"173","author":"Pacher","year":"2008","journal-title":"Am J Pathol"},{"key":"2021033116172689700_CIT0040","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/nrd1718","article-title":"Poly (ADP-ribose) polymerase and the therapeutic effects of its inhibitors","volume":"4","author":"Jagtap","year":"2005","journal-title":"Nat Rev Drug Discov"}],"container-title":["Bioscience, Biotechnology, and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bbb\/article-pdf\/84\/1\/134\/36835009\/bbb0134.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/bbb\/article-pdf\/84\/1\/134\/36835009\/bbb0134.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T11:48:57Z","timestamp":1654602537000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bbb\/article\/84\/1\/134\/5937599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,2]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1,2]]},"published-print":{"date-parts":[[2020,1,2]]}},"URL":"https:\/\/doi.org\/10.1080\/09168451.2019.1664893","relation":{},"ISSN":["0916-8451","1347-6947"],"issn-type":[{"value":"0916-8451","type":"print"},{"value":"1347-6947","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,1,2]]},"published":{"date-parts":[[2020,1,2]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=tbbb20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=tbbb20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"},{"value":"2019-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}