{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:44:37Z","timestamp":1790207077901,"version":"4.1.0"},"reference-count":31,"publisher":"Informa UK Limited","issue":"5","license":[{"start":{"date-parts":[[2020,5,3]],"date-time":"2020-05-03T00:00:00Z","timestamp":1588464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,5,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro.<\/jats:p>","DOI":"10.1080\/09168451.2020.1713043","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T13:58:13Z","timestamp":1578578293000},"page":"936-942","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":18,"title":["Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues"],"prefix":"10.1080","volume":"84","author":[{"given":"Junko","family":"Ohki","sequence":"first","affiliation":[{"name":"Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan"},{"name":"Department of Health and Nutrition, Tsukuba International University, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiko","family":"Sakashita","sequence":"additional","affiliation":[{"name":"Division of Reproductive Sciences, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eitaro","family":"Aihara","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and System Physiology, University of Cincinnati, Cincinnati, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiko","family":"Inaba","sequence":"additional","affiliation":[{"name":"Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironobu","family":"Uchiyama","sequence":"additional","affiliation":[{"name":"Genome Research Center, Tokyo University of Agriculture, Tokyo, 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