{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T17:57:35Z","timestamp":1793383055063,"version":"4.5.0"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic> T helper cell polarisation is important under chronic immune stimulatory conditions and drives the type of the evolving immune response. Mice treated with superantigens <jats:italic>in vivo<\/jats:italic> display strong effects on T<jats:sub>h<\/jats:sub> subset differentiation. The aim of the study was to detect the intrinsic capacity of T cells to polarise under various <jats:italic>ex vivo<\/jats:italic> conditions.<\/jats:p><jats:p><jats:italic>Methods:<\/jats:italic> Purified CD4<jats:sup>+<\/jats:sup> T cells obtained from superantigen\u2010treated mice were cultured under T<jats:sub>h<\/jats:sub> polarising conditions <jats:italic>in vitro<\/jats:italic>. By combining intracellular cytokine staining and subsequent flow cytometric analysis with quantitative cytokine measurements in culture supernatants by enzyme\u2010linked immunosorbent assay (ELISA), the differential T<jats:sub>h<\/jats:sub> polarising capacity of the treatment can be detected in a qualitative and quantitative manner.<\/jats:p><jats:p><jats:italic>Results and conclusions:<\/jats:italic> BALB\/c mice were shown to be biased to develop strong T<jats:sub>h<\/jats:sub>2 polarised immune responses using T<jats:sub>h<\/jats:sub>0 stimulation of purified CD4<jats:sup>+<\/jats:sup> T cells from phosphate\u2010buffered saline\u2010treated mice. Nevertheless, our analysis methodology convincingly showed that even in these mice, Toxic Shock Syndrome Toxin\u20101 treatment <jats:italic>in vivo<\/jats:italic> resulted in a significantly stronger T<jats:sub>h<\/jats:sub>1 polarising effect than control treatment. Our results indicate that populations of T<jats:sub>h<\/jats:sub> cells can be assessed individually for their differential T<jats:sub>h<\/jats:sub>1 or T<jats:sub>h<\/jats:sub>2 maturation capacity <jats:italic>in vivo<\/jats:italic> by analysing robust <jats:italic>in vitro<\/jats:italic> polarisation cultures combined with intracellular cytokine staining and ELISA.<\/jats:p>","DOI":"10.1080\/0929350310001619744","type":"journal-article","created":{"date-parts":[[2004,4,30]],"date-time":"2004-04-30T02:20:18Z","timestamp":1083291618000},"page":"285-292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["T helper cell polarisation as a measure of the maturation of the immune response"],"prefix":"10.1155","volume":"12","author":[{"given":"Scott B.","family":"Cameron","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen H.","family":"Stolte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony W.","family":"Chow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huub F. J.","family":"Savelkoul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2003\/863929.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/0929350310001619744","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T05:01:25Z","timestamp":1723093285000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/0929350310001619744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1080\/0929350310001619744"],"URL":"https:\/\/doi.org\/10.1080\/0929350310001619744","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]},"assertion":[{"value":"2003-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}