{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:57:27Z","timestamp":1773280647479,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003711","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["062027"],"award-info":[{"award-number":["062027"]}],"id":[{"id":"10.13039\/501100003711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p><jats:italic>Background:<\/jats:italic> Exposure to microorganisms elicts the production of cytokines. These soluble factors enhance several innate immune functions and regulate the ensuing specific immune response aimed at limiting the spread of infection.<\/jats:p><jats:p><jats:italic>Aim:<\/jats:italic> This study was undertaken to quantify the plasma levels of pro\u2010inflammatory cytokines during the course of primary <jats:italic>Listeria monocytogenes<\/jats:italic> and <jats:italic>Campylobacter jejuni<\/jats:italic> infection. Using an \n<jats:italic>in vivo<\/jats:italic> infection the relationship between endogenous cytokines and the bacterial number in the liver of infected animals was examined.<\/jats:p><jats:p><jats:italic>Methods:<\/jats:italic> C57BL\/6 mice were infected by the intraperitoneal route. At different time points we determined the number of colony\u2010forming units of bacteria in the liver of infected animals and paralled these with the plasma levels of interferon\u2010gamma (IFN\u2010\u03b3), tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) and interleukin\u20106 (IL\u20106) measured by enzyme \nimmunoassays.<\/jats:p><jats:p><jats:italic>Results:<\/jats:italic> L. monocytogenes infection lasted 10\u201311 days. IFN\u2010\u03b3 production occurred in the early phase but was more pronounced after day 4, following the appearance of specific immunity. The duration of experimental campylobacteriosis was 15 days. Early IFN\u2010\u03b3 production was not significant but a progressive rise of this cytokine in plasma was seen during the second week post infection. Mice produced measurable amounts of plasma TNF\u2010\u03b1 immediately after being given viable <jats:italic>L. monocytogenes<\/jats:italic>, peaking on day 2\u20133 when the greatest number of bacteria was present in the examined organs. During <jats:italic>C. jejuni<\/jats:italic> infection plasma TNF\u2010\u03b1 was produced in a similar manner, but the highest concentrations were found a few days later than in listeriosis, in correlation with the different course of campylobacteriosis. The quantity of IL\u20106 increased and decreased in concordance with clearance of \n<jats:italic>L. monocytogenes<\/jats:italic> and the clinical status of the animals. <jats:italic>C. jejuni<\/jats:italic> did not promote the induction of this cytokine. This is to some extent an unusual finding. With respect to the role of IL\u20106 in Th2 responses and antibody production, the appearance of this cytokine in campylobacteriosis was more expected.<\/jats:p><jats:p><jats:italic>Discussion:<\/jats:italic> During systemic bacterial infection, a network of pro\u2010inflammatory cytokines is activated and blood levels of these cytokines are elevated, albeit inconsistently, with large individual variations and depending on microbial characteristics and structure.<\/jats:p>","DOI":"10.1080\/09629350020025746","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T15:37:35Z","timestamp":1027697855000},"page":"229-234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Plasma cytokine response in mice with bacterial infection"],"prefix":"10.1155","volume":"9","author":[{"given":"Maja","family":"Abram","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darinka","family":"Vu\u010dkovi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Branka","family":"Wraber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miljenko","family":"Dori\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2000\/206097.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/09629350020025746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T13:41:41Z","timestamp":1723124501000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/09629350020025746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1080\/09629350020025746"],"URL":"https:\/\/doi.org\/10.1080\/09629350020025746","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"2000-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}