{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:10:13Z","timestamp":1761675013969},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>: Human peripheral blood mononuclear cells (PBMC) left in microcultures for 24 h without mitogen do not respond to subsequent stimulation with PHA. They regain reactivity if the native culture medium is absorbed with other party lymphocytes or partially replaced with the medium from a PHA\u2010stimulated culture. The observations suggest that, during the incubation, some inhibitory agent had accumulated in the culture medium.<\/jats:p><jats:p><jats:italic>Aim<\/jats:italic>: The study was performed to determine the nature of the observed phenomenon in respect of the possible role of monocytes and their products IL\u20101 and IL\u20101 receptor antagonist (IL\u20101ra), and to test for immunodiagnostic purposes the significance of quantifying the lymphocyte response to delayed stimulation with PHA in patients suffering from inflammatory prosesses.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: Lymphocyte response to delayed stimulation with PHA, calculated as the lymphocyte\u2010monokine interaction (LM) index, was determined in the microcultures of PBMC isolated from the blood of healthy donors or of patients with acute tonsilitis. The values of LM indices were compared with the ratios of IL\u20101ra\/IL\u20101\u03b2 concentration estimated by enzyme\u2010linked immunosorbent assay method in the culture supernatants. The influences of exogenous IL\u20101\u03b2 , IL\u20101ra, anti\u2010IL1ra antibodies and antibiotic cefaclor on the monokine concentrations and on the values of LM index were tested.<\/jats:p><jats:p><jats:italic>Results and conclusions<\/jats:italic>: The results show that the level of lymphocyte response to delayed stimulation with PHA (LM index) is inversely proportional to the ratio of IL\u20101ra\/IL\u20101\u03b2 concentration in the culture. The low LM values at high IL\u20101ra\/IL\u20101\u03b2 ratios in PBMC cultures from healthy donors, reversed proportions found in patients\u2032 PBMC (acute tonsilitis), and the cefaclor\u2010induced reduction of LM value with correlated increase of the IL\u20101ra\/IL\u20101\u03b2 ratio suggest that the LM assay may prove to be useful for immunodiagnostic purposes.<\/jats:p>","DOI":"10.1080\/09629350124376","type":"journal-article","created":{"date-parts":[[2003,11,25]],"date-time":"2003-11-25T20:37:08Z","timestamp":1069792628000},"page":"101-107","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Competition of IL\u20101 and IL\u20101ra determines lymphocyte response to delayed stimulation with PHA"],"prefix":"10.1155","volume":"10","author":[{"given":"Marek P.","family":"D\u0105browski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanda","family":"Stankiewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadeusz","family":"P\u0142usa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Chcia\u0142owski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanis\u0142aw","family":"Szmigielski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2001\/985976.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/09629350124376","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T04:29:03Z","timestamp":1723091343000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/09629350124376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1080\/09629350124376"],"URL":"https:\/\/doi.org\/10.1080\/09629350124376","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"type":"print","value":"0962-9351"},{"type":"electronic","value":"1466-1861"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2001-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}