{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T10:27:34Z","timestamp":1777717654332,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p><jats:italic>BACKGROUND<\/jats:italic>: Carrageenin (CAR) injection into the pleural cavity causes local inflammation called carrageenin\u2010induced pleurisy (CAR\u2010IP). Inflammation onset is characterized by an activation of pro\u2010inflammatory NF\u03baB, RelA\u2010p50, while inflammation resolution is characterized by an activation of an anti\u2010inflammatory NF\u03baB, p50\u2010p50, that re\u2010establishes homeostasis, an essential process for an organism\u2032s survival. Although chronic alcohol intake disrupts inflammation, the mechanism behind the development of inflammatory disorder in alcoholics is not yet known. Therefore, the aim of this investigation was to study the effects of ethanol intake on CAR\u2010IP and NF\u03baB activation in pleural fluid neutrophils in P rats.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: Alcohol\u2010preferring, P rats were given free choice of alcohol (15% ethanol) and water or water alone (for control) for 15 days. Then, each rat was injected with 0.2 ml of 2% CAR into the pleural cavity under light ether anesthesia. At different time intervals after the CAR injection, rats were anesthetized and their blood and pleural fluid samples were collected. Pleural fluid inflammatory cells were identified with Turk\u2032s or Wright\u2010Giemsa staining. Different cell types were sorted using a fluorescence\u2010activated cell sorter. Pleural fluid neutrophils were examined for apoptosis and activation of the two NF\u03baB subspecies.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: In control rats, fluid began to accumulate in the pleural cavity 0.5 h after, which peaked 24 h after, CAR injection. Then, the values declined gradually. The increase in pleural fluid correlated with RelA\u2010p50 activation, while the decline in pleural fluid correlated with p50\u2010p50 activation and apoptosis in neutrophils. In alcohol\u2010drinking rats, pleural fluid remained elevated for up to 6 days after CAR injection. Neutrophils from alcohol\u2010drinking rats exhibited suppressed apoptosis, augmented RelA\u2010p50 activation, and suppressed p50\u2010p50 activation.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>: Alcohol intake prolonged inflammation in P rats. An alcohol\u2010induced upregulation of RelA\u2010p50 activation and downregulation of p50\u2010p50 activation may be causally related to the alcohol\u2010induced inflammation dysregulation.<\/jats:p>","DOI":"10.1080\/09629350400003035","type":"journal-article","created":{"date-parts":[[2004,11,9]],"date-time":"2004-11-09T16:09:58Z","timestamp":1100016598000},"page":"255-262","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Differential activation of NF\u03baB\/RelA\u2010p50 and NF\u03baB\/p50\u2010p50 in control and alcohol\u2010drinking rats subjected to carrageenin\u2010induced pleurisy"],"prefix":"10.1155","volume":"13","author":[{"given":"Ashok K.","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2004\/935928.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/09629350400003035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T14:09:19Z","timestamp":1723126159000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/09629350400003035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1080\/09629350400003035"],"URL":"https:\/\/doi.org\/10.1080\/09629350400003035","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2004-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}