{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:32:15Z","timestamp":1758630735124},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p><jats:italic>BACKGROUND<\/jats:italic>: Peripheral blood CD8<jats:sup>+<\/jats:sup> T cells expressing interferon gamma and interleukin\u20104 (IL\u20104), and lacking CD28 molecules, were responsible for the dynamic interplay between peripheral blood and inflammatory sites.<\/jats:p><jats:p><jats:italic>Introduction<\/jats:italic>: The aim of the current study was to define in Beh\u00e7et\u2032s disease (BD), CD8<jats:sup>+<\/jats:sup> T\u2010cell subsets using CD28 and CD11b monoclonal antibodies, and the characterization of the Tc1\/Tc2 ratio and perforin expression.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: Flow cytometry was used for intracytoplasmic cytokines and perforin expression. Effector cells were investigated by adhesion of CD8<jats:sup>+<\/jats:sup> T cells to human microvascular endothelial cells and by chemotaxis using \u03b2\u2010chemokine.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: Interferon\u2010gamma\u2010producing CD8<jats:sup>+<\/jats:sup> T cells in active and remission BD patients were increased, which induce a significant increase of the Tc1:Tc2 ratio in BD. CD8<jats:sup>+<\/jats:sup>CD28<jats:sup>\u2212<\/jats:sup>CD11b<jats:sup>+<\/jats:sup> T cells were found to be more expanded in BD patients than in age\u2010matched healthy controls. The expression of CD11b molecules in active BD allowed to CD8<jats:sup>+<\/jats:sup>CD28<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>CD28<jats:sup>\u2212<\/jats:sup> subsets to adhere to human microvascular endothelial cells, with more efficiency in BD. Using MIP\u20101\u03b1, we observed that the migratory process of CD28<jats:sup>\u2212<\/jats:sup>CD11b<jats:sup>+<\/jats:sup> is more important in BD. CD28<jats:sup>\u2212<\/jats:sup>CD11b<jats:sup>+<\/jats:sup> exhibited an increased perforin expression in BD patients.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>: Taken together these results suggest the presence of immune activation, probably in response to a profound inflammation affecting BD patients. The physiopathological significance of these results were toward autoimmune diseases and\/or infectious process.<\/jats:p>","DOI":"10.1080\/09629350400003167","type":"journal-article","created":{"date-parts":[[2004,11,9]],"date-time":"2004-11-09T16:09:58Z","timestamp":1100016598000},"page":"247-253","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Tc1\/Tc2 ratio in the inflammatory process in patients with Beh\u00e7et\u2032s disease"],"prefix":"10.1155","volume":"13","author":[{"given":"Habib","family":"Houman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes","family":"Hamzaoui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imed Ben","family":"Ghorbal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monia Smiti","family":"Khanfir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moncef","family":"Feki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamel","family":"Hamzaoui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2004\/181607.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/09629350400003167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T14:09:21Z","timestamp":1723126161000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/09629350400003167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1080\/09629350400003167"],"URL":"https:\/\/doi.org\/10.1080\/09629350400003167","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"type":"print","value":"0962-9351"},{"type":"electronic","value":"1466-1861"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2004-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}