{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:03:42Z","timestamp":1785179022260,"version":"3.55.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p><jats:italic>Objective.<\/jats:italic> To compare a real\u2010time polymerase chain reaction (PCR) assay with broth culture for the detection of <jats:italic>Trichomonas vaginalis<\/jats:italic> using self\u2010collected vaginal swabs.<\/jats:p><jats:p><jats:italic>Methods.<\/jats:italic> Self\u2010collected vaginal swabs were obtained from adolescent and young adult African\u2010American women participating in HIV\u20101 prevention programs. <jats:italic>T. vaginalis<\/jats:italic> culture was performed using the InPouch TV System. Samples\nfor the real\u2010time PCR assay were collected using the BDProbeTec ET Culturette Direct Dry Swab system and tested in a laboratory\u2010developed assay which targeted a repeated sequence of the genome. Discrepant samples that were culture negative and positive in the real\u2010time PCR assay were tested in a confirmatory PCR which targeted a different region of the <jats:italic>T. vaginalis<\/jats:italic> genome, the18S ribosomal DNA gene.<\/jats:p><jats:p><jats:italic>Results.<\/jats:italic> Of the 524 specimens tested by both culture and real\u2010time PCR, 36 were culture positive and 54 were positive in the real\u2010time PCR assay; 16 of the 18 discrepant specimens were also positive in the confirmatory PCR assay. Using a\nmodified gold standard of positive by culture or positive in both PCR assays, the sensitivity of the real\u2010time PCR assay was 100% and the specificity was 99.6%, whereas culture had a sensitivity of 69.2% and a specificity of 100%.<\/jats:p><jats:p><jats:italic>Conclusions.<\/jats:italic> The real\u2010time PCR assay was sensitive and specific for the detection of <jats:italic>T. vaginalis<\/jats:italic> DNA from self\u2010collected vaginal swab specimens. The ability to use the BDProbeTec dry swab system for the real\u2010time PCR testing allowed for the detection of <jats:italic>Chlamydia trachomatis, Neisseria gonorrhoeae,<\/jats:italic> and <jats:italic>T. vaginalis<\/jats:italic> from a single specimen.<\/jats:p>","DOI":"10.1080\/10647440500068248","type":"journal-article","created":{"date-parts":[[2005,9,15]],"date-time":"2005-09-15T05:32:51Z","timestamp":1126762371000},"page":"145-150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Real\u2010Time PCR Improves Detection of <i>Trichomonas vaginalis<\/i> Infection Compared With Culture Using Self\u2010Collected Vaginal swabs"],"prefix":"10.1155","volume":"13","author":[{"given":"A. M.","family":"Caliendo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Jordan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Green","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ingersoll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Diclemente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. M.","family":"Wingood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,12]]},"container-title":["Infectious Diseases in Obstetrics and Gynecology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/idog\/2005\/942408.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/10647440500068248","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T20:51:46Z","timestamp":1723063906000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/10647440500068248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1080\/10647440500068248"],"URL":"https:\/\/doi.org\/10.1080\/10647440500068248","archive":["Portico"],"relation":{},"ISSN":["1064-7449"],"issn-type":[{"value":"1064-7449","type":"print"}],"subject":[],"published":{"date-parts":[[2004,12]]},"assertion":[{"value":"2004-07-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-12-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}