{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:04:04Z","timestamp":1773291844834,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,1,26]],"date-time":"2010-01-26T00:00:00Z","timestamp":1264464000000},"content-version":"vor","delay-in-days":1152,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:bold>Objective:<\/jats:bold> To investigate the role of a K<jats:sub>ATP<\/jats:sub> channel in sensing shear, specifically its cessation, in the endothelial cells of the pulmonary microvasculature.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Endothelial cells isolated from the pulmonary microvasculature of wild\u2010type and K<jats:sub>ATP<\/jats:sub> channel knockout (K<jats:sub>IR<\/jats:sub>6.2<jats:sup>\u2212\/\u2212<\/jats:sup>) mice were either statically cultured (non\u2010flow\u2010adapted) or kept under flow (flow\u2010adapted) and the K<jats:sub>IR<\/jats:sub> currents in these cells were monitored by whole\u2010cell patch\u2010clamp technique during flow and its cessation. Membrane potential changes, generation of reactive oxygen species (ROS), and Ca<jats:sup>2+<\/jats:sup> influx with flow cessation were evaluated by the use of fluorescent dyes. Lungs isolated from wild\u2010type mice were imaged to visualize ROS generation in the subpleural endothelium.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> By patch\u2010clamp analysis, reduction in the K<jats:sub>IR<\/jats:sub> current with cessation of flow occurred only in wild\u2010type cells that were flow\u2010adapted and not in flow\u2010adapted K<jats:sub>IR<\/jats:sub>6.2<jats:sup>\u2212\/\u2212<\/jats:sup> cells. Similar observations were made using changes in bisoxonol fluorescence as an index of cell membrane potential. Generation of ROS and Ca<jats:sup>2+<\/jats:sup> influx that follow membrane depolarization were significantly lower in statically cultured and in K<jats:sub>IR<\/jats:sub>6.2<jats:sup>\u2212\/\u2212<\/jats:sup> cells as compared to flow\u2010adapted wild\u2010type cells. Imaging of subpleural endothelial cells of the whole lung showed that the K<jats:sub>ATP<\/jats:sub> antagonist glyburide caused the production of ROS in the absence of flow cessation.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The responses to stop of flow (viz. membrane depolarization, K<jats:sub>IR<\/jats:sub> currents, ROS, Ca<jats:sup>2+<\/jats:sup>) were significantly altered with knockout of K<jats:sub>ATP<\/jats:sub> channels, which indicates that this channel is an important component of the pulmonary endothelial response to abrupt loss of shear stress.<\/jats:p>","DOI":"10.1080\/10739680600930255","type":"journal-article","created":{"date-parts":[[2006,11,3]],"date-time":"2006-11-03T19:12:57Z","timestamp":1162581177000},"page":"633-644","source":"Crossref","is-referenced-by-count":61,"title":["K<sub><b>ATP<\/b><\/sub> Channels Are an Important Component of the Shear\u2010Sensing Mechanism in the Pulmonary Microvasculature"],"prefix":"10.1111","volume":"13","author":[{"given":"S.","family":"CHATTERJEE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"LEVITAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"WEI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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