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The aim was to determine whether depletion of KC through pretreatment with GdCl<jats:sub>3<\/jats:sub>or MDP alters the hepatic microcirculation during lipopolysaccharide (LPS)\u2010induced shock in rats and to test if there are substance\u2010specific differences.<jats:bold>Methods:<\/jats:bold>Rats received either MDP or GdCl<jats:sub>3<\/jats:sub>or saline prior to induction of LPS shock. Hepatic microcirculation was evaluated by intravital microscopy (sinusoidal diameter, sinusoidal bloodflow, leukocyte adhesion), and the gene expression in the hepatic non\u2010parenchymal cell fraction was determined by RT\u2010PCR.<jats:bold>Results:<\/jats:bold>GdCl<jats:sub>3<\/jats:sub>pretreatment prevented sinusoidal narrowing but did not restore sinusoidal blood flow and did not normalize leukocyte\u2010endothelial interaction time after LPS. In contrast, MDP pretreatment improved hepatic microcirculation consistently for all parameters measured compared to GdCl<jats:sub>3<\/jats:sub>pretreated animals. In the non\u2010parenchymal cell fraction, eNOS gene expression was preserved and gene expression of TNF\u2010\u03b1 was blocked after MDP but not after GdCl<jats:sub>3<\/jats:sub>application prior to LPS shock.<jats:bold>Conclusions:<\/jats:bold>The results show that GdCl<jats:sub>3<\/jats:sub>and MDP cannot be used equivalently for experimental KC depletion in the condition of LPS\u2010induced shock. These findings should be taken into consideration in studies that evaluate the role of Kupffer cells in models of endotoxin\u2010induced shock.<\/jats:p>","DOI":"10.1080\/10739680701675213","type":"journal-article","created":{"date-parts":[[2009,12,8]],"date-time":"2009-12-08T20:57:17Z","timestamp":1260305837000},"page":"427-439","source":"Crossref","is-referenced-by-count":9,"title":["Differential Effects of GdCl<sub>3<\/sub>\u2010 or MDP Treatment on Rat Liver Microcirculation and Gene Expression in the Hepatic Non\u2010Parenchymal Cell Fraction in LPS 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