{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:30:18Z","timestamp":1741753818872,"version":"3.38.0"},"reference-count":28,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Toxicol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p> The authors studied mineralocorticoid receptor (MCR)-mediated effects of steroids on CD34<jats:sup>+<\/jats:sup> progenitor cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed the presence of m RNA for both the MCR and the alpha subunit of the epithelial sodium channel, a member of the amiloride-sensitive sodium channel (ASSC) superfamily, in human CD41<jats:sup>+<\/jats:sup> megacaryoblastic cells derived from cultured bone marrow CD34<jats:sup>+<\/jats:sup> isolates, as well as in the human erythromegakaryoblastic leukemia (HEL) cell line. Immunofluorescence also revealed the presence of both the MCR and ASSC in circulating CD34<jats:sup>+<\/jats:sup> and medullar CD41<jats:sup>+<\/jats:sup> megacaryoblastic cells, the former as a nucleocy-toplasmic protein and the latter as a membrane-bound protein, as expected from earlier studies using MCR-specific targets. In a selective medium, the formation of erythrocyte burst-forming units, and of the granulocyte-macrophage colony-forming units, by circulating CD34<jats:sup>+<\/jats:sup> cells was influenced by the agonists deoxycorti-costerone and aldosterone, as well as by the antagonists RU 26752 and ZK 91587, targeted for the MCR. The multiplication of the leukemic HEL progeny, derived from CD41<jats:sup>+<\/jats:sup> cells, was similarly altered by these steroids targeted for the MCR. In contrast, in the optimal growth medium, the multiplication, and colony formation by bone marrow CD34<jats:sup>+<\/jats:sup> progenitor cells were not altered by either aldosterone or ZK 91587. These and other studies reveal that the receptor-mediated action of mineralocorticoids may influence the functional maturation of the hematopoietic progenitor lineage, contrary to the classical notion where the mineralotropic effect would be a unique feature of the epithelial cell. <\/jats:p>","DOI":"10.1080\/10915810305118","type":"journal-article","created":{"date-parts":[[2003,9,18]],"date-time":"2003-09-18T22:40:18Z","timestamp":1063924818000},"page":"297-304","source":"Crossref","is-referenced-by-count":1,"title":["Mineralocorticoid Hormones Exert Dramatic Effects on Pluripotent Human Stem Cell Progeny"],"prefix":"10.1177","volume":"22","author":[{"given":"M.","family":"Mirshahi","sequence":"first","affiliation":[{"name":"INSERM and CNRS, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Valamanesh","sequence":"additional","affiliation":[{"name":"INSERM and CNRS, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Golestaneh","sequence":"additional","affiliation":[{"name":"INSERM and CNRS, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Mirshahi","sequence":"additional","affiliation":[{"name":"INSERM and CNRS, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Vincent","sequence":"additional","affiliation":[{"name":"INSERM and CNRS, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Tang","sequence":"additional","affiliation":[{"name":"INSERM and CNRS, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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