{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:03:07Z","timestamp":1789538587925,"version":"build-2803163510"},"reference-count":42,"publisher":"Informa UK Limited","issue":"1","license":[{"start":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T00:00:00Z","timestamp":1757203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T00:00:00Z","timestamp":1757203200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1080\/policies-text-data-mining"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity"},{"name":"Key Laboratory of Jiangxi Province for Functional Biology and Pollution Control in Red Soil Regions"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":["Journal of Enzyme Inhibition and Medicinal Chemistry"],"published-print":{"date-parts":[[2025,12,31]]},"DOI":"10.1080\/14756366.2025.2554899","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T03:05:53Z","timestamp":1757300753000},"update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":2,"title":["Production and functional characteristics of a novel hirudin variant with better anticoagulant activities than bivalirudin"],"prefix":"10.1080","volume":"40","author":[{"given":"Peijuan","family":"Ge","sequence":"first","affiliation":[{"name":"School of Life Sciences, Jinggangshan University","place":["Ji\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jinggangshan University","place":["Ji\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zimo","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jinggangshan University","place":["Ji\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Licheng","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jinggangshan University","place":["Ji\u2019an, China"]},{"name":"Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, Jinggangshan University","place":["Ji\u2019an, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"301","published-online":{"date-parts":[[2025,9,7]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232112772"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389557519666191015201125"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.13780"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.52752"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2022.119793"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmr.2025.100037"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11203214"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-019-0347-0"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7291075"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/02648725.2018.1506898"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10924"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.05.69"},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.14533"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.138533"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7716"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.26616"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2019-205922"},{"key":"e_1_3_5_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.660757"},{"key":"e_1_3_5_20_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S353088"},{"key":"e_1_3_5_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12871-018-0475-y"},{"key":"e_1_3_5_22_1","volume-title":"StatPearls","author":"Berlioz BE","year":"2025","unstructured":"Berlioz BE, Patel P, Sanghavi, DK, Bivalirudin. StatPearls. Treasure Island (FL): StatPearls Publishing Stat Pearls Publishing LLC; 2025."},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-0061-0"},{"key":"e_1_3_5_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc0382"},{"key":"e_1_3_5_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10080606"},{"key":"e_1_3_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-32303-2"},{"key":"e_1_3_5_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-76715-w"},{"key":"e_1_3_5_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes14112068"},{"key":"e_1_3_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.08.003"},{"key":"e_1_3_5_30_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20210600"},{"key":"e_1_3_5_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201310777"},{"key":"e_1_3_5_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.3c00388"},{"key":"e_1_3_5_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48211-6"},{"key":"e_1_3_5_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-020-10835-1"},{"issue":"01","key":"e_1_3_5_35_1","first-page":"1","article-title":"Prokaryotic expression and purification of recombinant hirudin","volume":"45","author":"Kongwei H","year":"2018","unstructured":"Kongwei H, Zhipeng L, Kuiqing C, et al. Prokaryotic expression and purification of recombinant hirudin. China Anim Husb Vet Med. 2018;45(01):1\u201313.","journal-title":"China Anim Husb Vet Med"},{"key":"e_1_3_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2021.03.012"},{"key":"e_1_3_5_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-024-08411-x"},{"issue":"01","key":"e_1_3_5_38_1","first-page":"47","article-title":"Study on anticoagulant activities of extracts in vitro from dried and fresh materials of Whitmania pigra","volume":"31","author":"Ming W","year":"2012","unstructured":"Ming W, Shihuan T, Yun Y, et al. Study on anticoagulant activities of extracts in vitro from dried and fresh materials of Whitmania pigra. J South Cent Minzu Univ Nat Sci Ed. 2012;31(01):47\u201350.","journal-title":"J South Cent Minzu Univ Nat Sci Ed"},{"key":"e_1_3_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11239-021-02456-w"},{"issue":"5","key":"e_1_3_5_40_1","first-page":"830","article-title":"Bivalirudin","volume":"99","author":"Warkentin TE","year":"2008","unstructured":"Warkentin TE, Greinacher A, Koster A. Bivalirudin. Thromb Haemost. 2008;99(5):830\u2013839.","journal-title":"Thromb Haemost"},{"key":"e_1_3_5_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-3175-1_24"},{"key":"e_1_3_5_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-14-6"},{"key":"e_1_3_5_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27275-8"}],"container-title":["Journal of Enzyme Inhibition and Medicinal Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.tandfonline.com\/doi\/pdf\/10.1080\/14756366.2025.2554899","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.tandfonline.com\/doi\/full-xml\/10.1080\/14756366.2025.2554899","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.tandfonline.com\/doi\/pdf\/10.1080\/14756366.2025.2554899","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:15:02Z","timestamp":1789535702000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.tandfonline.com\/doi\/full\/10.1080\/14756366.2025.2554899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,7]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,12,31]]}},"alternative-id":["10.1080\/14756366.2025.2554899"],"URL":"https:\/\/doi.org\/10.1080\/14756366.2025.2554899","relation":{},"ISSN":["1475-6366","1475-6374"],"issn-type":[{"value":"1475-6366","type":"print"},{"value":"1475-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,7]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=ienz20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=ienz20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"},{"value":"2025-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-07","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2554899"}}