{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:37:27Z","timestamp":1773657447057,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,1,3]],"date-time":"2008-01-03T00:00:00Z","timestamp":1199318400000},"content-version":"vor","delay-in-days":1828,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Selective cloning of the cDNA coding for a weak neurotoxin (WTX) from cobra N. kaouthia including the 5'\u2010 and 3'\u2010non\u2010translated regions (NTR) is described. The known amino acid sequence of WTX was used together with the nucleotide sequence of a weak neurotoxin NNAM2 from cobra Naja atra, to design WTX\u2010specific primers for direct amplification of an internal WTX cDNA fragment by RT\u2010PCR. The sequence of the complete WTX cDNA was determined in sequencing runs on internal PCR products, cloned 3'\u2010 and 5'\u2010RACE\u2010fragments and several full\u2010length cDNA clones. The cDNA coding sequence is in excellent agreement with the previously determined WTX amino acid sequence, has a high homology with other known weak toxin cDNAs, whereas even higher homology (up to 96%) with several classes of 3\u2010finger toxins was detected in the 59 bp 3'\u2010NTR consensus sequence. A possible function of the highly conserved nucleotide sequence elements is discussed. IUBMB Life, 55: 43\u201047, 2003<\/jats:p>","DOI":"10.1080\/1521654021000061664","type":"journal-article","created":{"date-parts":[[2003,11,25]],"date-time":"2003-11-25T23:01:17Z","timestamp":1069801277000},"page":"43-47","source":"Crossref","is-referenced-by-count":3,"title":["Direct Cloning of a Target Gene from a Pool of Homologous Sequences: Complete cDNA Sequence of a Weak Neurotoxin from Cobra Naja kaouthia"],"prefix":"10.1002","volume":"55","author":[{"given":"TL","family":"Oustitch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u2010E.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Utkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"VI","family":"Tsetlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,3]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F1521654021000061664","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/1521654021000061664","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T15:12:07Z","timestamp":1700233927000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/1521654021000061664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1080\/1521654021000061664"],"URL":"https:\/\/doi.org\/10.1080\/1521654021000061664","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]}}}