{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:56:43Z","timestamp":1792177003089,"version":"4.4.0"},"reference-count":0,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,1,3]],"date-time":"2008-01-03T00:00:00Z","timestamp":1199318400000},"content-version":"vor","delay-in-days":1585,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>From a liver cDNA library prepared from a nonvenomous striated snake, Elaphe quadrivirgata, we isolated a cDNA encoding a novel protein, PLI\u03b1\u2010like protein (PLI\u03b1\u2010LP), having approximately 70% sequence identities with the \u03b1\u2010type phospholipase A2 (PLA2) inhibitors (PLI\u03b1s) previously purified from the venomous snakes Agkistrodon blomhoffii siniticus and Trimeresurus flavoviridis. Since the PLI\u03b1\u2010LP with a highly conserved C\u2010type lectin\u2010like domain (CTLD) would be predicted to function as a PLA2 inhibitor, we purified this protein from E. quadrivirgata serum by sequential chromatography on Hi\u2010trap Blue, Mono Q, and Superdex 200 columns. The purified 51\u2010kDa protein with PLI\u03b1\u2010like immunoreactivity was found to be a trimer of 18\u2010kDa PLI\u03b1\u2010LP, which was comparable to the trimeric structure of PLI\u03b1. But, unexpectedly, this protein did not show any inhibitory activity against various snake venom PLA2s. Furthermore, it did not inhibit the endogenous PLA2 activities in various tissue homogenates prepared from this snake. Lack of the inhibitory activity in PLI\u03b1\u2010LP may provide important information concerning the structure\u2010function relationships of PLI\u03b1. IUBMB Life, 55: 539\u2010545, 2003<\/jats:p>","DOI":"10.1080\/15216540310001620995","type":"journal-article","created":{"date-parts":[[2004,4,30]],"date-time":"2004-04-30T02:34:34Z","timestamp":1083292474000},"page":"539-545","source":"Crossref","is-referenced-by-count":17,"title":["Identification and Characterization of a Serum Protein Homologous to \u03b1\u2010type Phospholipase A2 Inhibitor (PLI\u03b1) from a Nonvenomous Snake, Elaphe quadrivirgata"],"prefix":"10.1002","volume":"55","author":[{"given":"Kohji","family":"Okumura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyozo","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,1,3]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216540310001620995","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216540310001620995","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T22:00:57Z","timestamp":1700172057000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216540310001620995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["10.1080\/15216540310001620995"],"URL":"https:\/\/doi.org\/10.1080\/15216540310001620995","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9]]}}}