{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T13:42:49Z","timestamp":1698500569145},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,1,16]],"date-time":"2008-01-16T00:00:00Z","timestamp":1200441600000},"content-version":"vor","delay-in-days":4216,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1996,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mechanism by which chlorpromazine (2\u2010chloro\u201010\u2010(3\u2010dimethylaminopropyl)\u2010phenothiazine) reverses P\u2010glycoprotein (P\u2010gp2)mediated multidrug resistance was investigated using membrane vesicles prepared from human CCRF\u2010CEM leukaemia cells. Chlorpromazine was transported in an ATP\u2010dependent manner into membrane vesicles prepared from vinblastine resistant (VBL1000) cells but not from drug\u2010sensitive cells. The chlorpromazine uptake was sensitive to osmotic pressure, indicating true transport into the vesicle lumen. The ATP\u2010dependent chlorpromazine uptake was inhibited about 30% by the addition of ammonium chloride, indicating that a pH or electrochemical gradient could not account for the majority of ATP\u2010dependent chlorpromazine uptake. The results of this study show that chlorpromazine is actively transported by P\u2010glycoprotein and that chemosensitization by phenothiazines may occur by competition of these agents for active transport of anticancer agents by P\u2010glycoprotein.<\/jats:p>","DOI":"10.1080\/15216549600201761","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:37:17Z","timestamp":1183808237000},"page":"687-696","source":"Crossref","is-referenced-by-count":1,"title":["Chlorpromazine transport in membrane vesicles from multidrug resistant CCRF\u2010CEM cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Samiuddin K.","family":"Syed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard I.","family":"Christopherson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basil D.","family":"Roufogalis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,16]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549600201761","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549600201761","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T03:39:24Z","timestamp":1698377964000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549600201761"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,7]]}},"alternative-id":["10.1080\/15216549600201761"],"URL":"https:\/\/doi.org\/10.1080\/15216549600201761","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7]]}}}