{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T04:36:07Z","timestamp":1717043767312},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,1,16]],"date-time":"2008-01-16T00:00:00Z","timestamp":1200441600000},"content-version":"vor","delay-in-days":4063,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The capacity of oxidative metabolism and its regulation is an important factor in disease control. Using scopoletin as a fluorescent substrate of peroxidase the extent of menadione\u2010dependent production of H2O2 by rat thymocytes was determined. The reaction was inhibited by 2,4\u2010dinitrophenol, papaverine, nordihydroguaiaretic acid and iodacetamide. The membrane\u2010penetrating SH reagent N\u2010ethylmaleimide primed the reaction, probably due to an inhibition of glutathione peroxidase. To delineate an influence of cell\u2010surface protein\u2010carbohydrate interactions by exogenous lectins, the impact of cell binding was analyzed for several plant lectins, namely concanavalin A, phytohemagglutinin, the lectins from Triticum vulgaris and from Sambucus nigra. Except for the \u03b1\u2010NeuNAc(2\u20106)gal\/galNAc\u2010binding agglutinin, the other three plant proteins with specificities to different parts of N\u2010linked oligosaccharides primed the reaction. This activity of lectins did not coincide with their ability to aggregate cells. The given results indicate that biosignaling pathways triggered by lectins are involved in the regulation of the intracellular reduction of menadione.<\/jats:p>","DOI":"10.1080\/15216549600201783","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:39:09Z","timestamp":1183808349000},"page":"1149-1158","source":"Crossref","is-referenced-by-count":1,"title":["Effects of metabolic inhibitors and lectins on the menadione\u2010dependent generation of H2O2 by rat thymocytes"],"prefix":"10.1002","volume":"40","author":[{"given":"A. V.","family":"Timoshenko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. N.","family":"Loiko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. N.","family":"Cherenkevich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. \u2010J.","family":"Gabius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,16]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549600201783","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549600201783","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T13:22:07Z","timestamp":1698326527000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549600201783"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,12]]}},"alternative-id":["10.1080\/15216549600201783"],"URL":"https:\/\/doi.org\/10.1080\/15216549600201783","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,12]]}}}