{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:50:30Z","timestamp":1780091430036,"version":"3.54.0"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T00:00:00Z","timestamp":1200528000000},"content-version":"vor","delay-in-days":4002,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1997,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of proline on the prevention of trichloroacetic acid (TCA)\u2010induced protein precipitation is studied. It is found that proline at high concentrations (&gt;4.0 M) completely prevents TCA\u2010induced precipitation of hen egg white lysozyme. Other osmolytes such as ethylene glycol, glycerol and sucrose fail to prevent the TCA\u2010induced precipitation of lysozyme. Viscosity and 1\u2010anilino\u20108\u2010naphthalene sulphonic acid binding experiments suggest that proline at high concentration forms an ordered supramolecular assembly. Proline is shown to increase the solubility of protein due to formation of such higher order assemblies. A model of the supra\u2010molecular assembly of proline is proposed and a possible in vivo role of the increased levels of proline under water stress is discussed.<\/jats:p>","DOI":"10.1080\/15216549700201241","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:39:31Z","timestamp":1183808371000},"page":"235-242","source":"Crossref","is-referenced-by-count":16,"title":["Proline is a protein solubilizing solute"],"prefix":"10.1002","volume":"41","author":[{"given":"D.","family":"Samuel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. K. S.","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Jayaraman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. W.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,1,17]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549700201241","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549700201241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T22:21:09Z","timestamp":1698445269000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549700201241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,2]]}},"alternative-id":["10.1080\/15216549700201241"],"URL":"https:\/\/doi.org\/10.1080\/15216549700201241","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,2]]}}}