{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T15:13:23Z","timestamp":1698506003127},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T00:00:00Z","timestamp":1200528000000},"content-version":"vor","delay-in-days":3760,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Signaling processes in the course of the formation of the lectin\u2010mediated aggregates may partake in conveying enhanced stability to the cell clusters. To prove the validity of this reasoning in a model, we have studied the impact of addition of three metabolic inhibitors (N\u2010ethylmaleimide, nordihydroguaiaretic acid, and trifluoperazine on lactose\u2010dependent dissociation of cell aggregates, formed in the presence of the galactoside\u2010binding mistletoe lectin. Using both human neutrophils and rat thymocytes to avoid measurement of responses restricted to a single cell type, an enhanced dissociation of lectin\u2010formed cell aggregates was observed, when lactose and an inhibitor were present. Among the tested inhibitors, nordihydroguaiaretic acid and N\u2010ethylmateimide were more potent enhancers of cell dissociation than trifluoperazine. These results suggest that biosignaling pathways connected with lipoxygenase activity as well as the level of intracellular sulfhydryl groups confer further stability to lectin\u2010dependent cell aggregates. The systematic evaluation of inhibitors for defined activities is thus suggested as a tool to disclose the nature and the contribution of individual signaling mechanisms to post\u2010binding effects following lectin\u2010initiated cell contact formation.<\/jats:p>","DOI":"10.1080\/15216549700204271","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:42:11Z","timestamp":1183808531000},"page":"477-487","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic inhibitors as tools to delineate participation of distinct intracellular pathways in enhancement of lactose\u2010induced dissociation of neutrophil and thymocyte aggregates formed by mediation of a plant lectin"],"prefix":"10.1002","volume":"43","author":[{"given":"A. V.","family":"Timoshenko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. V.","family":"Gorudko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kaltner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. N.","family":"Cherenkevich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. \u2010J.","family":"Gabius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,17]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549700204271","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549700204271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T23:50:14Z","timestamp":1698450614000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549700204271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,10]]}},"alternative-id":["10.1080\/15216549700204271"],"URL":"https:\/\/doi.org\/10.1080\/15216549700204271","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10]]}}}