{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T23:10:42Z","timestamp":1698534642874},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T00:00:00Z","timestamp":1200528000000},"content-version":"vor","delay-in-days":3609,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have investigated the effect of cross\u2010linking on the enzymatic activity and oligomer formation of the chicken stomach ecto\u2010apyrase. Cross\u2010linking with the hydrophobic, lysine\u2010specific dithiobis(succinimidylpropionate) (DSP) caused equal inhibition of ATPase and ADPase activity in both the membrane\u2010bound and detergent\u2010solubilized ecto\u2010apyrase. The inhibitory effect of cross\u2010linking was reversed upon the addition of the reductant dithiothreitol. Western blots of aliquots of the cross\u2010linked samples show decreased amounts of the monomeric 80 kDa ecto\u2010apyrase and the appearance of a 160 kDa dimer under conditions inducing enzyme inhibition. Therefore, the chicken stomach ecto\u2010apyrase, like the chicken gizzard ecto\u2010ATPase, is likely a homodimer in vivo. Unlike the related gizzard ecto\u2010ATPase, however, the native stomach ecto\u2010apyrase is not stimulated, but rather inhibited by cross\u2010linking, presumably due to different quaternary structural stability of the two enzymes.<\/jats:p>","DOI":"10.1080\/15216549800201482","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:43:37Z","timestamp":1183808617000},"page":"463-470","source":"Crossref","is-referenced-by-count":0,"title":["Cross\u2010linking induces homodimer formation and inhibits enzymatic activity of chicken stomach ecto\u2010aryrase"],"prefix":"10.1002","volume":"44","author":[{"given":"Stephanie Ann Lewis","family":"Carl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas M.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terence L.","family":"Kirley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,17]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549800201482","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549800201482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T22:31:00Z","timestamp":1698532260000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549800201482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1080\/15216549800201482"],"URL":"https:\/\/doi.org\/10.1080\/15216549800201482","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]}}}