{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T04:59:18Z","timestamp":1698555558258},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T00:00:00Z","timestamp":1200528000000},"content-version":"vor","delay-in-days":3517,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The complement\u2010mediated lysis of guinea pig erythrocytes by cobra venom factor (CVF) decreased by 50\u201060% within 2 min of treatment with 5 mM sodium periodate at 0 \u00b0C. This loss of activity paralleled modification of 3\u20104 Met; other amino acids and sugar residues of the oligosaccharide chains were not affected. Treatment with N\u2010chlorosuccinimide or chloramine\u2010T under conditions that specifically modified 3\u20104 readily\u2010oxidizable Met also caused 50\u201060% loss of CVF activity. The secondary structure of CVF was not altered by these modifications. Methionine\u2010modified CVF (MetCVF) supported the cleavage of factor B by factor D with equal efficiency as that of untreated CVF to form C3\/C5 convertase (MetCVF,Bb) of the alternative pathway. MetCVF,Bb and CVF,Bb were indistinguishable with respect to C3 cleavage. However, the C5\u2010cleavage ability of MetCVF,Bb was significantly lower than that of CVF,Bb. These results suggest the involvement of Met in CVF binding of C5.<\/jats:p>","DOI":"10.1080\/15216549800202502","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:44:44Z","timestamp":1183808684000},"page":"133-144","source":"Crossref","is-referenced-by-count":1,"title":["Methionine modification impairs the C5\u2010cleavage function of cobra venom factor\u2010dependent C3\/C5 convertase"],"prefix":"10.1002","volume":"45","author":[{"given":"Qinglan","family":"Fu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Channe","family":"Gowda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"McPhie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,17]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549800202502","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549800202502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T16:29:20Z","timestamp":1698510560000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549800202502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,6]]}},"alternative-id":["10.1080\/15216549800202502"],"URL":"https:\/\/doi.org\/10.1080\/15216549800202502","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,6]]}}}