{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T05:11:21Z","timestamp":1698556281530},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T00:00:00Z","timestamp":1200528000000},"content-version":"vor","delay-in-days":3425,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of eicosapentaenoic acid and its 15\u2010hydroperoxy and 15\u2010hydroxy adducts on the activity of glucosamine synthetase, the rate\u2010limiting enzyme of mucus synthesis, in rabbit gastric antral mucosa were examined. 15\u2010Hydroperoxy\u2010eicosapentaenoic acid inhibited the glucosamine synthetase activity at concentrations of 10, 20 and 50 \u03bcM. The effect was concentration\u2010dependent, and the concentration required for 50% inhibition was approximately 20 \u03bcM. Eicosapentaenoic acid and its 15\u2010hydroxy adduct had no significant effect on the enzyme activity at the same concentration range. The experiment utilizing Fe2+ revealed that the inhibitory effect of 15\u2010hydroperoxyeicosapentaenoic acid on the glucosamine synthetase activity is not due to hydroxy radical which is expected to be formed from the hydroperoxy adduct. These results suggest that 15\u2010hydroperoxyeicosapentaenoic acid has the potential to reduce the synthesis of gastric mucus by inhibiting the glucosamine synthetase activity.<\/jats:p>","DOI":"10.1080\/15216549800203662","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:45:55Z","timestamp":1183808755000},"page":"157-163","source":"Crossref","is-referenced-by-count":0,"title":["Effects of eicosapentaenoic acid and its 15\u2010hydroperoxy and 15\u2010hydroxy derivatives on glucosamine synthetase activity in rabbit gastric mucosa"],"prefix":"10.1002","volume":"46","author":[{"given":"Tadashi","family":"Fujita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Sakuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naho","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohko","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,17]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549800203662","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549800203662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T20:21:16Z","timestamp":1698524476000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549800203662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,9]]}},"alternative-id":["10.1080\/15216549800203662"],"URL":"https:\/\/doi.org\/10.1080\/15216549800203662","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9]]}}}