{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T22:30:43Z","timestamp":1720737043713},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T00:00:00Z","timestamp":1200528000000},"content-version":"vor","delay-in-days":3364,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prostaglandin D synthetase (PGD\u2010S; prostaglandin\u2010H2 D\u2010isomerase, EC 5,3,99,2), a 30 kDa glycoprotein also known as \u03b2\u2010trace protein that catalyzes the formation of prostaglandin D2 (PGD2) from PGH2, was purified to apparent homogeneity from human cerebrospinal fluid (CSF) using a two\u2010step procedure involving HPLC on a Vydac C8 reversed\u2010phase column and high performance electrophoresis chromatography (HPEC) using a 10% T SDS\u2010polyacrylamide gel. The purity of PGD\u2010S isolated from CSF was confirmed by silver stained SDS\u2010polyacrylamide gel and direct protein microsequencing (NH2\u2010APEAQVSVQPNFQ). A highly specific polyclonal antibody was prepared against this protein for immunoassay development. Using an ELISA, it was found that the concentration of PGD\u2010S in CSF did not alter significantly in different pathological conditions of the central nervous system (CNS). These include dementia (n=9), hydrocephalus (n=4), neuropathy (n=11), optic neuritis (n=4), multiple sclerosis (n=11), and demyelinating syndrome (n=11), when compared to normal individuals (n=12); however, the level of PGD\u2010S in the CSF obtained from patients with brain tumor (n=11), was reduced by as much as 2\u2010fold when compared to control samples (n=12) illustrating PGD\u2010S is a potentially useful marker for brain tumor.<\/jats:p>","DOI":"10.1080\/15216549800204172","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:46:32Z","timestamp":1183808792000},"page":"643-656","source":"Crossref","is-referenced-by-count":4,"title":["Quantification of prostaglandin D synthetase in cerebrospinal fluid: A potential marker for brain tumor"],"prefix":"10.1002","volume":"46","author":[{"given":"Luciano","family":"Saso","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grazia","family":"Leone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Sorrentino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Giacomelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Silvestrini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josephine","family":"Grima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan C. H.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen","family":"Samy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolores","family":"Mruk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Yan","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,17]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F15216549800204172","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15216549800204172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T20:40:59Z","timestamp":1698525659000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/15216549800204172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,11]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,11]]}},"alternative-id":["10.1080\/15216549800204172"],"URL":"https:\/\/doi.org\/10.1080\/15216549800204172","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,11]]}}}