{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T20:20:49Z","timestamp":1726431649962},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p>A positive correlation has been established\nbetween increased oxidative stress and cardiovascular\ndiseases in diabetes mellitus. We evaluated\nthe effects of single or combined treatments\nwith vitamin A (retinol acetate, 30\nmg\/kg\/day, for 12\u2010weeks) and insulin (8\u201010\nIU\/rat\/day for the final 6\u2010week) on vasomotor\nactivity, oxidative stress and retinol metabolism\nin 12\u2010week streptozotocin diabetic rats. The\nvasomotor activity was determined by measuring\nin <jats:italic>vitro<\/jats:italic> responsiveness of aorta rings to\nphenylephrine (PE) and acetylcholine (ACh) in\nthe absence or in the presence of hydrogen peroxide\n(H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>). Preincubation with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> (10\n\u03bcM) produced a significant decrease in PE (1\nmM)\u2010induced contraction in untreated\u2010diabetic\nbut not in control rats. Single treatment with\ninsulin counteracted this effect of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and\nalso reversed the increased contractile response\nof diabetic aorta to PE, while vitamin A was\nfound to be ineffective. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> (10 \u03bcM) also\ninhibited ACh (1 mM)\u2010stimulated endothelium\u2010\ndependent relaxation two fold more in diabetic\nthan in control aorta. In the prevention of\nH<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced inhibition of vascular relaxation\nto ACh, vitamin A alone was markedly effective\nwhile insulin alone was not. The combination\nof vitamin A plus insulin removed the\ninhibitory action of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in diabetic aorta.\nDiabetic animals displayed an increased level of aorta thiobarbituric acid reactive substance\n(TBARS) in association with decreased levels of\nplasma retinol and retinol\u2010binding protein\n(RBP). Single treatment with insulin, in spite of\nallowing recovery of normal growth rate and\nimproved glucose and retinol metabolism in\ndiabetic rats, was unable to control TBARS\nproduction to the same extent as vitamin A\nalone. Our findings suggest that the maintenance\nof ACh\u2010stimulated endothelium\u2010dependent\nvasorelaxant tone in normal physiological\nlevels depends largely on the prevention and\/or\ninhibition of peroxidative stress, which is\nachieved by combined treatment with vitamin\nA plus insulin. The use of vitamin A together\nwith insulin provides a better metabolic control\nand more benefits than use of insulin alone in\nthe reduction of diabetes\u2010induced vascular\ncomplications.<\/jats:p>","DOI":"10.1080\/15604280214484","type":"journal-article","created":{"date-parts":[[2003,11,11]],"date-time":"2003-11-11T16:00:48Z","timestamp":1068566448000},"page":"119-130","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Hydrogen Peroxide\u2010Induced Inhibition of Vasomotor Activity: Evaluation of Single and Combined Treatments With Vitamin A and Insulin in Streptozotocin\u2010Diabetic Rats"],"prefix":"10.1155","volume":"3","author":[{"given":"Fulya","family":"Zobali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanju","family":"Besler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuray","family":"Ari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c7imen","family":"Karasu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,11,20]]},"container-title":["Journal of Diabetes Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2002\/817290.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/15604280214484","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:05:11Z","timestamp":1723071911000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/15604280214484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11,20]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1080\/15604280214484"],"URL":"https:\/\/doi.org\/10.1080\/15604280214484","archive":["Portico"],"relation":{},"ISSN":["2314-6745","2314-6753"],"issn-type":[{"type":"print","value":"2314-6745"},{"type":"electronic","value":"2314-6753"}],"subject":[],"published":{"date-parts":[[2001,11,20]]},"assertion":[{"value":"2001-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-11-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}