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Fifteen healthy males (23.3 \u00b1 2.7 years, 170.3 \u00b1 6.5\u2005cm, 64.9 \u00b1 8.7\u2005kg) received three different interventions, in random order. Intervention 1 received 10\u2005min of aerobic exercise after five cycles of one minute of static stretching. Intervention 2 received 10\u2005min of aerobic exercise before the static stretching. Intervention 3 received 5\u2005min of aerobic exercise both before and after the static stretching. The range of motion of ankle dorsiflexion, stretch tolerance, muscle tendon unit stiffness, peak torque of ankle plantarflexion, and the amplitude of electromyography were measured. In all interventions, the range of motion and stretch tolerance significantly increased (<jats:italic>p<\/jats:italic> &lt; 0.05), but muscle tendon unit stiffness decreased significantly for all interventions (<jats:italic>p<\/jats:italic> &lt; 0.05). Peak torque of ankle plantar flexion and amplitude of electromyography significantly increased for Interventions 1 and 3 (<jats:italic>p<\/jats:italic> &lt; 0.05), while these significantly decreased for Intervention 2 (<jats:italic>p<\/jats:italic> &lt; 0.05). These data indicated that range of motion and stretch tolerance were increased, but muscle tendon unit stiffness was decreased regardless of the order of static stretching and aerobic exercise. Aerobic exercise after static stretching increased the peak torque and amplitude of electromyography.<\/jats:p>","DOI":"10.1080\/17461391.2020.1866079","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T22:54:43Z","timestamp":1608245683000},"page":"297-303","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":11,"title":["The effects of using a combination of static stretching and aerobic exercise on muscle tendon unit stiffness and strength in ankle plantar\u2010flexor muscles"],"prefix":"10.1002","volume":"22","author":[{"given":"Kosuke","family":"Takeuchi","sequence":"first","affiliation":[{"name":"Faculty of Rehabilitation Kobe International University Kobe Japan"},{"name":"Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Takemura","sequence":"additional","affiliation":[{"name":"Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiko","family":"Tsukuda","sequence":"additional","affiliation":[{"name":"Faculty of Sport Biwako Seikei Sport College Otsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumpei","family":"Miyakawa","sequence":"additional","affiliation":[{"name":"Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/168.6.442"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","unstructured":"Behm D. 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