{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:54:36Z","timestamp":1790128476184,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007903","name":"Bloodwise","doi-asserted-by":"publisher","award":["13067"],"award-info":[{"award-number":["13067"]}],"id":[{"id":"10.13039\/501100007903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["278514"],"award-info":[{"award-number":["278514"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007903","name":"Bloodwise","doi-asserted-by":"publisher","award":["13067"],"award-info":[{"award-number":["13067"]}],"id":[{"id":"10.13039\/501100007903","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union\u2019s Seventh Framework Programme for Research, Technological development and demonstration","award":["278514"],"award-info":[{"award-number":["278514"]}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extracellular vesicles have been described in non\u2010paracrine cellular interactions in cancer. We report a similar phenomenon in B\u2010cell precursor (BCP) acute lymphoblastic leukaemia (ALL). Using advanced microscopy and high throughput screening, we further characterise a subset of large vesicles (LEVs) identified in cell lines, murine models of human BCP\u2010ALL and clinical samples. Primary ALL blasts and cell lines released heterogeneous anucleate vesicles &lt;6\u00a0micron into extracellular fluids. Larger LEVs were enclosed in continuous membranes, contained intact organelles and demonstrated an organised cytoskeleton. An excess of circulating CD19\u2010positive LEVs were observed in diagnostic samples and isolated from mice engrafted with BCP\u2010ALL primary cells. LEVs exhibited dynamic shape change\n                    <jats:italic>in vitro<\/jats:italic>\n                    and were internalised by other leukaemic cell lines leading to phenotypic transformation analogous to the cell of origin. In patient\u2010derived xenografts, LEVs were released by primary ALL cells into extracellular spaces and internalised by murine mesenchymal cells\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Collectively these data highlight the heterogeneity but accessibility of LEVs in clinical samples and their potential to provide a unique insight into the biology of the cell of origin and to their development as novel biomarkers to aid diagnosis and improve therapeutic outcomes.\n                  <\/jats:p>","DOI":"10.1080\/20013078.2017.1294339","type":"journal-article","created":{"date-parts":[[2017,3,10]],"date-time":"2017-03-10T04:08:50Z","timestamp":1489118930000},"update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":44,"title":["Acute lymphoblastic leukaemia cells produce large extracellular vesicles containing organelles and an active cytoskeleton"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7295-3036","authenticated-orcid":false,"given":"Suzanne M.","family":"Johnson","sequence":"first","affiliation":[{"name":"Children's Cancer Group Division of Molecular and Clinical Cancer Sciences School of Medical Sciences Faculty of Biology, Medicine and Health University of Manchester Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare","family":"Dempsey","sequence":"additional","affiliation":[{"name":"Children's Cancer Group Division of Molecular and Clinical Cancer Sciences School of Medical Sciences Faculty of Biology, Medicine and Health University of Manchester Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catriona","family":"Parker","sequence":"additional","affiliation":[{"name":"Children's Cancer Group Division of Molecular and Clinical Cancer Sciences School of Medical Sciences Faculty of Biology, Medicine and Health University of Manchester Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1497-328X","authenticated-orcid":false,"given":"Aleksandr","family":"Mironov","sequence":"additional","affiliation":[{"name":"Children's Cancer Group Division of Molecular and Clinical Cancer Sciences School of Medical Sciences Faculty of Biology, Medicine and Health University of Manchester Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4246-9830","authenticated-orcid":false,"given":"Helen","family":"Bradley","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Medicine and Health CRUK Manchester Institute University of Manchester Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2916-9649","authenticated-orcid":false,"given":"Vaskar","family":"Saha","sequence":"additional","affiliation":[{"name":"Children's Cancer Group Division of Molecular and Clinical Cancer Sciences School of Medical Sciences Faculty of Biology, Medicine and Health University of Manchester Manchester UK"},{"name":"Tata Translational Cancer Research Centre Tata Medical Center Kolkata India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12]]},"reference":[{"issue":"2","key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ijms17020170","article-title":"Focus on extracellular vesicles: introducing the next small big thing","volume":"17","author":"Kalra H","year":"2016","journal-title":"Int J Mol 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