{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:48:08Z","timestamp":1773298088744,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T00:00:00Z","timestamp":1569542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T00:00:00Z","timestamp":1569542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":["J of Extracellular Vesicle"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Extracellular vesicles (EVs) play central physiological and pathophysiological roles in intercellular communication. Biomarker studies addressing disorders such as cardiovascular diseases often focus on circulating microRNAs (miRNAs) and may, depending on the type of disease and clinic routine, utilise patient specimens sampled from arterial or venous blood vessels. Thus, it is essential to test whether circulating miRNA profiles depend on the respective sampling site. We assessed potential differences in arterial and venous cell\u2010free miRNA profiles in a cohort of 20 patients scheduled for cardiac surgery. Prior to surgery, blood was simultaneously sampled from the radial artery and the internal jugular vein. After precipitating crude EVs, we performed small RNA Sequencing, which failed to detect significantly regulated miRNAs using stringent filtering criteria for differential expression analysis. Filtering with less strict criteria, we detected four miRNAs slightly upregulated in arterial samples, one of which could be validated by reverse transcription real\u2010time PCR. The applicability of these findings to purified arterial and venous EVs was subsequently tested in a subset of the initial study population. While an additional clean\u2010up step using size\u2010exclusion chromatography seemed to reduce overall miRNA yield compared to crude EV samples, no miRNAs with differential arteriovenous expression were detected. Unsupervised clustering approaches were unable to correctly classify samples drawn from arteries or veins based on miRNAs in either crude or purified preparations. Particle characterisation of crude preparations as well as characterisation of EV markers in purified EVs resulted in highly similar characteristics for arterial and venous samples. With the exception of specific pathologies (e.g. severe pulmonary disorders), arterial versus venous blood sampling should therefore not represent a likely confounder when studying differentially expressed circulating miRNAs. The use of either arterial or venous serum EV samples should result in highly similar data on miRNA expression profiles for the majority of biomarker studies.<\/jats:p><jats:p><jats:bold>Abbreviations<\/jats:bold> ACE inhibitors: Angiotensin\u2010converting\u2010enzyme inhibitors; ApoA1: Apolipoprotein A1; CNX: Calnexin; Cv: Coefficient of variation; cDNA: Complementary DNA; CABG: Coronary artery bypass graft; DGE: Differential gene expression; DPBS: Dulbecco's Phosphate Buffered Saline; EVs: Extracellular vesicles; log2FC: Log2 fold change; baseMean: Mean miRNA expression; miRNA: MicroRNA; NTA: Nanoparticle Tracking Analysis; NGS: Next\u2010Generation Sequencing; RT\u2010qPCR: Reverse transcription quantitative real\u2010time PCR; rRNA: Ribosomal RNA; RT: Room temperature; SEC: Size\u2010exclusion chromatography; snoRNA: Small nucleolar RNA; snRNA: Small nuclear RNA; small RNA\u2010Seq: Small RNA Sequencing; SD: Standard deviation; tRNA: Transfer RNA; TEM: Transmission electron microscopy; UA: Uranyl acetate.<\/jats:p>","DOI":"10.1080\/20013078.2019.1670935","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T08:24:23Z","timestamp":1569572663000},"update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":25,"title":["Transcriptomic profiling of cell\u2010free and vesicular microRNAs from matched arterial and venous sera"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6274-5919","authenticated-orcid":false,"given":"Stefanie","family":"Hermann","sequence":"first","affiliation":[{"name":"Division of Animal Physiology and Immunology School of Life Sciences Weihenstephan Technical University of Munich Freising Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0460-6459","authenticated-orcid":false,"given":"Dominik","family":"Buschmann","sequence":"additional","affiliation":[{"name":"Division of Animal Physiology and Immunology School of Life Sciences Weihenstephan Technical University of Munich Freising Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3878-0148","authenticated-orcid":false,"given":"Benedikt","family":"Kirchner","sequence":"additional","affiliation":[{"name":"Division of Animal Physiology and Immunology School of Life Sciences Weihenstephan Technical University of Munich Freising Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0741-616X","authenticated-orcid":false,"given":"Melanie","family":"Borrmann","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University Hospital Ludwig\u2010Maximilians\u2010University Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3741-287X","authenticated-orcid":false,"given":"Florian","family":"Brandes","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University Hospital Ludwig\u2010Maximilians\u2010University Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Kotschote","sequence":"additional","affiliation":[{"name":"IMGM Laboratories GmbH Planegg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Bonin","sequence":"additional","affiliation":[{"name":"IMGM Laboratories GmbH Planegg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Lindemann","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics University Hospital Ludwig\u2010Maximilians\u2010University Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9113-9643","authenticated-orcid":false,"given":"Marlene","family":"Reithmair","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics University Hospital Ludwig\u2010Maximilians\u2010University Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6538-0652","authenticated-orcid":false,"given":"Gustav","family":"Schelling","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University Hospital Ludwig\u2010Maximilians\u2010University Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3192-1019","authenticated-orcid":false,"given":"Michael W.","family":"Pfaffl","sequence":"additional","affiliation":[{"name":"Division of Animal Physiology and Immunology School of Life Sciences Weihenstephan Technical University of Munich Freising Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,27]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1704286"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002770050214"},{"key":"e_1_2_8_4_2","first-page":"21","article-title":"MicroRNAomes of porcine arterial and venous blood","volume":"13","author":"Bai L","year":"2014","journal-title":"J Anim Vet Adv"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.15310"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.2-114.v1"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1481321"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4185"},{"key":"e_1_2_8_10_2","unstructured":"AndrewsS.FastQC: a quality control tool for high throughput sequence data.2010. Available online at:http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2011.05.009"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw545"},{"key":"e_1_2_8_13_2","doi-asserted-by":"crossref","first-page":"D123","DOI":"10.1093\/nar\/gku991","article-title":"RNAcentral: an international database of ncRNA sequences","volume":"43","author":"RN C","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1181"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-3-r25"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13162"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01386-z"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1596016"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7NR08360B"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0167969"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.08.082"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-02-0618"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2012.689038"},{"key":"e_1_2_8_28_2","first-page":"1133","article-title":"Evaluation of methodologies for microRNA biomarker detection by next generation sequencing","volume":"15","author":"Coenen\u2010Stass AML","year":"2018","journal-title":"RNA Biol"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2016.1270005"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019055108"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2210"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr254"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep24316"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-018-2773-4"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep09430"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041561"}],"container-title":["Journal of Extracellular Vesicles"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/20013078.2019.1670935","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T17:33:08Z","timestamp":1760635988000},"score":1,"resource":{"primary":{"URL":"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1080\/20013078.2019.1670935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,27]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1080\/20013078.2019.1670935"],"URL":"https:\/\/doi.org\/10.1080\/20013078.2019.1670935","archive":["Portico"],"relation":{},"ISSN":["2001-3078","2001-3078"],"issn-type":[{"value":"2001-3078","type":"print"},{"value":"2001-3078","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,27]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=zjev20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=zjev20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"},{"value":"2019-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-14","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1670935"}}