{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:21:39Z","timestamp":1759332099092},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,1,18]],"date-time":"2008-01-18T00:00:00Z","timestamp":1200614400000},"content-version":"vor","delay-in-days":2939,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously found a novel glycosylphosphatidylinositol (GPI)\u2010 anchored glycoprotein designated GPI\u201080 that modulates complement receptor 3 integrin\u2010dependent adhesion and in vitro transendothelial migration of neutrophils. In this study, we show that antibody\u2010mediated cross\u2010linking of GPI\u201080 led to rapid tyrosine phosphorylation mainly of a 34\u2010kDa protein (pp34). Chemical inhibitors, such as genistein, sodium orthovanadate, wortmannin, cytochalasin B, Ro 31\u20108220, and 1,2\u2010bis(2\u2010aminophenoxy)ethane\u2010 N, N, N, N,\u2010tetraacetic acid inhibited this response, whereas pertussis toxin had no effect. These findings demonstrate that the tyrosine phosphorylation of pp34 by cross\u2010linking GPI\u201080 in human neutrophils involves tyrosine kinases, tyrosine phosphatases, phosphatidylinositol 3\u2010kinase, cytoskeleton reorganization, protein kinase C, and cytoplasmic calcium, but not heterotrimeric G proteins.<\/jats:p>","DOI":"10.1080\/713803588","type":"journal-article","created":{"date-parts":[[2007,7,7]],"date-time":"2007-07-07T11:49:34Z","timestamp":1183808974000},"page":"43-47","source":"Crossref","is-referenced-by-count":9,"title":["Tyrosine Phosphorylation of a 34\u2010kDa Protein Induced by Cross\u2010Linking a Novel Glycosylphosphatidylinositol\u2010Anchored Glycoprotein (GPI\u201080) on Human Neutrophils That May Regulate Their Adherence and Migration"],"prefix":"10.1002","volume":"49","author":[{"given":"Yan","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Araki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fujiro","family":"Sendo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,18]]},"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F713803588","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/713803588","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T15:44:28Z","timestamp":1698594268000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1080\/713803588"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1080\/713803588"],"URL":"https:\/\/doi.org\/10.1080\/713803588","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]}}}