{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:07:15Z","timestamp":1761674835190},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["BM\/19156\/99"],"award-info":[{"award-number":["BM\/19156\/99"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p><jats:italic>Aims:<\/jats:italic> Annexin I (ANXA1), a 37 kDa member of the annexin family of Ca<jats:sup>2+<\/jats:sup>\u2010binding and phospholipidbinding proteins, is particularly abundant in various populations of peripheral blood leukocytes. Since this protein modulates the anti\u2010inflammatory actions of the steroid hormones, the purpose of this study was to investigate the effects of the female sex steroid hormone, 17\u03b2\u2010estradiol (E<jats:sub>2<\/jats:sub>\u03b2), on the synthesis and secretion of ANXA1 in the human CCRF\u2010CEM acute lymphoblastic leukemia cell line.<\/jats:p><jats:p><jats:italic>Methods:<\/jats:italic> Complementary reverse transcription\u2010polymerase chain reaction and Western blot assays were performed to study the effect of E<jats:sub>2<\/jats:sub>\u03b2 on the expression of mRNA and protein ANXA1, respectively.<\/jats:p><jats:p><jats:italic>Results and discussion:<\/jats:italic> Treatment of CCRF\u2010CEM cells with E<jats:sub>2<\/jats:sub>\u03b2, for 30 min, stimulated the synthesis of ANXA1 mRNA molecules, and increased the cellular level of ANXA1 protein. Moreover, when the cells were incubated with E<jats:sub>2<\/jats:sub>\u03b2, under the same experimental conditions, a significant increase in the amount of ANXA1 secreted from the cells was also detected. ICI 182,780, a selective inhibitor of the intracellular estrogen receptor, had no effect on the E<jats:sub>2<\/jats:sub>\u03b2\u2010stimulated expression and externalisation of ANXA1. Taken together, these results indicate that E<jats:sub>2<\/jats:sub>\u03b2 induces <jats:italic>de novo<\/jats:italic> synthesis of ANXA1 and stimulates its secretion in the CCRF\u2010CEM cell line, apparently through a mechanism independent of the intracellular estrogen receptor.<\/jats:p>","DOI":"10.1080\/09629350120093713","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T15:32:07Z","timestamp":1027697527000},"page":"245-251","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["17\u03b2\u2010Estradiol promotes the synthesis and the secretion of annexin I in the CCRF\u2010CEM human cell line"],"prefix":"10.1155","volume":"10","author":[{"given":"Margarida","family":"Castro-Caldas","sequence":"first","affiliation":[]},{"given":"Carlos B.","family":"Duarte","sequence":"additional","affiliation":[]},{"given":"Ars\u00e9lio P.","family":"Carvalho","sequence":"additional","affiliation":[]},{"given":"M. Celeste","family":"Lopes","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2001\/738560.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/09629350120093713","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:23:41Z","timestamp":1723073021000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/09629350120093713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1080\/09629350120093713"],"URL":"https:\/\/doi.org\/10.1080\/09629350120093713","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"type":"print","value":"0962-9351"},{"type":"electronic","value":"1466-1861"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2001-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}