{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T10:49:45Z","timestamp":1773053385291,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:p><jats:italic>Aims:<\/jats:italic> Glucocorticoids (GCs) exert some of their anti\u2010inflammatory actions by preventing the activation of the transcription factor nuclear factor (NF)\u2010\u03baB. The GC\u2010dependent inhibition of NF\u2010\u03baB may occur at different levels, but the mechanisms involved are still incompletely understood. In this work, we investigated whether the synthetic GC, dexamethasone (Dex), modulates the activity of NF\u2010\u03baB in the lymphoblastic CCRF\u2010CEM cell line. We also evaluated the ability of Dex to prevent the activation of NF\u2010\u03baB in response to the potent proinflammatory cytokine, interleukin (IL)\u20101\u03b2.<\/jats:p><jats:p><jats:italic>Results:<\/jats:italic> Exposure of the cells to Dex (1 \u03bcM) induced the rapid degradation of I\u03baB\u2010\u03b1, leading to the transient translocation of the NF\u2010\u03baB family members p65 and p50 from the cytoplasm to the nucleus, as evaluated by western blot. Electrophoretic mobility shift assays revealed that, in the nucleus, these NF\u2010\u03baB proteins formed protein\u2010DNA complexes, indicating a transient activation of NF\u2010\u03baB. Additionally, Dex also induced de novo synthesis of I\u03baB\u2010\u03b1, following its degradation. Finally, when the cells were exposed to Dex (1 \u03bcM) prior to stimulation with IL\u20101\u03b2 (20 ng\/ml), Dex was efficient in preventing IL\u20101\u03b2\u2010induced NF\u2010\u03baB activation. The GC antagonist, RU 486 (10 \u03bcM), did not prevent any of the effects of Dex reported here.<\/jats:p><jats:p><jats:italic>Conclusion:<\/jats:italic> Our results indicate that, in CCRF\u2010CEM cells, Dex prevents NF\u2010\u03baB activation, induced by IL\u20101\u03b2, by a mechanism that involves the upregulation of I\u03baB\u2010\u03b1 synthesis, and that depends on the early and transient activation of NF\u2010\u03baB.<\/jats:p>","DOI":"10.1080\/0962935031000096953","type":"journal-article","created":{"date-parts":[[2004,6,28]],"date-time":"2004-06-28T23:48:36Z","timestamp":1088466516000},"page":"37-46","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Dexamethasone prevents interleukin\u20101\u03b2\u2010induced nuclear factor\u2010\u03baB activation by upregulating I\u03baB\u2010\u03b1 synthesis, in lymphoblastic cells"],"prefix":"10.1155","volume":"12","author":[{"given":"M.","family":"Castro-caldas","sequence":"first","affiliation":[]},{"given":"A. F.","family":"Mendes","sequence":"additional","affiliation":[]},{"given":"A. P.","family":"Carvalho","sequence":"additional","affiliation":[]},{"given":"C. B.","family":"Duarte","sequence":"additional","affiliation":[]},{"given":"M. C.","family":"Lopes","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2003,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2003\/676735.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/0962935031000096953","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:27:44Z","timestamp":1723116464000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1080\/0962935031000096953"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1080\/0962935031000096953"],"URL":"https:\/\/doi.org\/10.1080\/0962935031000096953","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]},"assertion":[{"value":"2003-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}