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Still, they will act in conjunction with other released parasite\u2010derived products that might modify the EVs effects. Notwithstanding, the immunomodulatory properties of these non\u2010vesicular components and their influence in the infectious process remains unknown. To address this, we explored both in vitro and in vivo the immunomodulatory potential of promastigotes extracellular material (EXO), obtained as a whole or separated in two different fractions: EVs or vesicle depleted EXO (VDE). Using an air pouch model, we observed that EVs and VDE induced a dose\u2010dependent cell recruitment profile different from the one obtained with parasites, attracting significantly fewer neutrophils and more dendritic cells (DCs). Additionally, when we co\u2010inoculated parasites with extracellular products a drop in cell recruitment was observed. 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