{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:12:33Z","timestamp":1770822753661,"version":"3.50.1"},"reference-count":41,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,6,15]]},"abstract":"<jats:p>OCI-5\/GPC3 is a member of the glypican family. Glypicans are heparan sulfate proteoglycans that are bound to the cell surface through a glycosyl-phosphatidylinositol anchor. It has recently been shown that the OCI-5\/GPC3 gene is mutated in patients with the Simpson-Golabi-Behmel Syndrome (SGBS), an X-linked disorder characterized by pre- and postnatal overgrowth and various visceral and skeletal dysmorphisms. Some of these dysmorphisms could be the result of deficient growth inhibition or apoptosis in certain cell types during development. Here we present evidence indicating that OCI-5\/GPC3 induces apoptosis in cell lines derived from mesothelioma (II14) and breast cancer (MCF-7). This induction, however, is cell line specific since it is not observed in NIH 3T3 fibroblasts or HT-29 colorectal tumor cells. We also show that the apoptosis-inducing activity in II14 and MCF-7 cells requires the anchoring of OCI-5\/GPC3 to the cell membrane. The glycosaminoglycan chains, on the other hand, are not required. MCF-7 cells can be rescued from OCI-5\/GPC3\u2013induced cell death by insulin-like growth factor 2. This factor has been implicated in Beckwith-Wiedemann, an overgrowth syndrome that has many similarities with SGBS. The discovery that OCI-5\/GPC3 is able to induce apoptosis in a cell line\u2013 specific manner provides an insight into the mechanism that, at least in part, is responsible for the phenotype of SGBS patients.<\/jats:p>","DOI":"10.1083\/jcb.141.6.1407","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:47:50Z","timestamp":1027702070000},"page":"1407-1414","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":146,"title":["OCI-5\/GPC3, a Glypican Encoded by a Gene That Is Mutated in the Simpson-Golabi-Behmel Overgrowth Syndrome, Induces Apoptosis in a Cell Line\u2013specific Manner"],"prefix":"10.1083","volume":"141","author":[{"given":"Alfonso Due\u00f1as","family":"Gonzalez","sequence":"first","affiliation":[{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"},{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"}]},{"given":"Mitsunori","family":"Kaya","sequence":"additional","affiliation":[{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"},{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"}]},{"given":"Wen","family":"Shi","sequence":"additional","affiliation":[{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"},{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"}]},{"given":"Howard","family":"Song","sequence":"additional","affiliation":[{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"},{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"}]},{"given":"Joseph R.","family":"Testa","sequence":"additional","affiliation":[{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"}]},{"given":"Linda Z.","family":"Penn","sequence":"additional","affiliation":[{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"},{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"}]},{"given":"Jorge","family":"Filmus","sequence":"additional","affiliation":[{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"},{"name":"*Division of Cancer Biology Research, Sunnybrook Health Science Centre, Toronto, Ontario M4N 3M5, Canada; \u2021Department of Medical Biophysics, University of Toronto, Ontario M5G 2M9, Canada; \u00a7Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111; and \u2016Ontario Cancer Institute, Ontario M5G 2M9, Canada"}]}],"member":"291","published-online":{"date-parts":[[1998,6,15]]},"reference":[{"key":"2023072207505336900_B1","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1126\/science.2144057","article-title":"Suppression of human colorectal carcinoma cell growth by wild-type p53","volume":"249","author":"Baker","year":"1990","journal-title":"Science"},{"key":"2023072207505336900_B2","doi-asserted-by":"crossref","first-page":"12415","DOI":"10.1074\/jbc.272.19.12415","article-title":"Identification of glypican as a dual modulator of the biological activity of fibroblast growth factors","volume":"272","author":"Bonneh-Barkay","year":"1997","journal-title":"J Biol Chem"},{"key":"2023072207505336900_B3","doi-asserted-by":"crossref","first-page":"8756","DOI":"10.1073\/pnas.94.16.8756","article-title":"Resistance to apoptosis caused by PIG-A gene mutations in paroxysmal nocturnal hemoglobinuria","volume":"94","author":"Brodsky","year":"1997","journal-title":"Proc 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