{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T20:13:16Z","timestamp":1769631196076,"version":"3.49.0"},"reference-count":73,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,3,8]]},"abstract":"<jats:p>Alignment of the mitotic spindle with the axis of cell division is an essential process in Saccharomyces cerevisiae that is mediated by interactions between cytoplasmic microtubules and the cell cortex. We found that a cortical protein, the yeast formin Bni1p, was required for spindle orientation. Two striking abnormalities were observed in bni1\u0394 cells. First, the initial movement of the spindle pole body (SPB) toward the emerging bud was defective. This phenotype is similar to that previously observed in cells lacking the kinesin Kip3p and, in fact, BNI1 and KIP3 were found to be in the same genetic pathway. Second, abnormal pulling interactions between microtubules and the cortex appeared to cause preanaphase spindles in bni1\u0394 cells to transit back and forth between the mother and the bud. We therefore propose that Bni1p may localize or alter the function of cortical microtubule-binding sites in the bud. Additionally, we present evidence that other bipolar bud site determinants together with cortical actin are also required for spindle orientation.<\/jats:p>","DOI":"10.1083\/jcb.144.5.947","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"947-961","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":138,"title":["Control of Mitotic Spindle Position by the <i>Saccharomyces cerevisiae<\/i> Formin Bni1p"],"prefix":"10.1083","volume":"144","author":[{"given":"Laifong","family":"Lee","sequence":"first","affiliation":[{"name":"*Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; \u2021Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; \u00a7Institut f\u00fcr Biochemie, Universit\u00e4t Stuttgart, 70569 Stuttgart, Germany; and \u00b6Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"},{"name":"*Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; \u2021Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; \u00a7Institut f\u00fcr Biochemie, Universit\u00e4t Stuttgart, 70569 Stuttgart, Germany; and \u00b6Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"}]},{"given":"Saskia K.","family":"Klee","sequence":"additional","affiliation":[{"name":"*Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; \u2021Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; \u00a7Institut f\u00fcr Biochemie, Universit\u00e4t Stuttgart, 70569 Stuttgart, Germany; and \u00b6Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"},{"name":"*Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; \u2021Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; \u00a7Institut f\u00fcr Biochemie, Universit\u00e4t Stuttgart, 70569 Stuttgart, Germany; and \u00b6Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"}]},{"given":"Marie","family":"Evangelista","sequence":"additional","affiliation":[{"name":"*Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; \u2021Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; \u00a7Institut f\u00fcr Biochemie, Universit\u00e4t Stuttgart, 70569 Stuttgart, Germany; and \u00b6Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"}]},{"given":"Charles","family":"Boone","sequence":"additional","affiliation":[{"name":"*Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; \u2021Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; \u00a7Institut f\u00fcr Biochemie, Universit\u00e4t Stuttgart, 70569 Stuttgart, Germany; and \u00b6Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"}]},{"given":"David","family":"Pellman","sequence":"additional","affiliation":[{"name":"*Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; \u2021Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; \u00a7Institut f\u00fcr Biochemie, Universit\u00e4t Stuttgart, 70569 Stuttgart, Germany; and \u00b6Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6"}]}],"member":"291","published-online":{"date-parts":[[1999,3,8]]},"reference":[{"key":"2023072900343410100_B1","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1091\/mbc.8.4.729","article-title":"Aip3p\/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites","volume":"8","author":"Amberg","year":"1997","journal-title":"Mol Biol Cell"},{"key":"2023072900343410100_B2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1083\/jcb.137.2.399","article-title":"High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor Latrunculin-A","volume":"137","author":"Ayscough","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B3","first-page":"1295","article-title":"Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae.","volume":"11","author":"Bender","year":"1991","journal-title":"Mol Cell Biol"},{"key":"2023072900343410100_B4","doi-asserted-by":"crossref","first-page":"5264","DOI":"10.1128\/MCB.16.10.5264","article-title":"ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae.","volume":"10","author":"Bi","year":"1996","journal-title":"Mol Cell Biol"},{"key":"2023072900343410100_B5","doi-asserted-by":"crossref","first-page":"4661","DOI":"10.1093\/nar\/20.17.4661","article-title":"Identification of a tRNAGln ochre suppressor in Saccharomyces cerevisiae.","volume":"20","author":"Boone","year":"1992","journal-title":"Nucleic Acids Res"},{"key":"2023072900343410100_B6","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1101\/gad.8.9.1087","article-title":"Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae.","volume":"8","author":"Burns","year":"1994","journal-title":"Genes Dev"},{"key":"2023072900343410100_B7","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1091\/mbc.7.2.225","article-title":"Signals from the spindle midzone are required for the stimulation of cytokinesis in cultured epithelial cells","volume":"7","author":"Cao","year":"1996","journal-title":"Mol Biol Cell"},{"key":"2023072900343410100_B8","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1083\/jcb.138.3.629","article-title":"Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex","volume":"138","author":"Carminati","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B9","doi-asserted-by":"crossref","first-page":"3367","DOI":"10.1242\/dev.120.12.3367","article-title":"diaphanous is required for cytokinesis in Drosophila and shares domains of similarity with the products of the limb deformitygene","volume":"120","author":"Castrillon","year":"1994","journal-title":"Development (Camb)"},{"key":"2023072900343410100_B10","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1083\/jcb.137.1.169","article-title":"cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin","volume":"137","author":"Chang","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B11","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0955-0674(96)80035-4","article-title":"Generation of cell polarity in yeast","volume":"8","author":"Chant","year":"1996","journal-title":"Curr Opin Cell Biol"},{"key":"2023072900343410100_B12","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1083\/jcb.129.3.751","article-title":"Patterns of bud-site selection in the yeast Saccharomyces cerevisiae.","volume":"129","author":"Chant","year":"1995","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B13","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/0092-8674(95)90035-7","article-title":"Cleavage orientation and the asymmetric inheritance of Notch1 immunoreactivity in mammalian neurogenesis","volume":"82","author":"Chenn","year":"1995","journal-title":"Cell"},{"key":"2023072900343410100_B14","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1083\/jcb.138.5.1041","article-title":"Mitotic spindle positioning in Saccharomyces cerevisiaeis accomplished by antagonistically acting microtubule motor proteins","volume":"138","author":"Cottingham","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B15","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1101\/gad.9.15.1817","article-title":"Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast","volume":"9","author":"Cvrckova","year":"1995","journal-title":"Genes Dev"},{"key":"2023072900343410100_B16","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1083\/jcb.138.5.1023","article-title":"Kinesin-related KIP3 of Saccharomyces cerevisiaeis required for a distinct step in nuclear migration","volume":"138","author":"DeZwaan","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B17","first-page":"123","article-title":"Amplification with arbitrary primers","volume":"54","author":"Di Rienzo","year":"1996","journal-title":"Methods Mol Biol"},{"key":"2023072900343410100_B18","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1091\/mbc.4.12.1277","article-title":"Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site","volume":"4","author":"Drubin","year":"1993","journal-title":"Mol Biol Cell"},{"key":"2023072900343410100_B19","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0092-8674(00)81278-7","article-title":"Origins of cell polarity","volume":"84","author":"Drubin","year":"1996","journal-title":"Cell"},{"key":"2023072900343410100_B20","doi-asserted-by":"crossref","first-page":"2482","DOI":"10.1101\/gad.9.20.2482","article-title":"cappuccino, a Drosophila maternal effect gene required for polarity of the egg and embryo, is related to the vertebrate limb deformitylocus","volume":"9","author":"Emmons","year":"1995","journal-title":"Genes Dev"},{"key":"2023072900343410100_B21","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1083\/jcb.101.1.96","article-title":"Evidence for an involvement of actin in the positioning and motility of centrosomes","volume":"101","author":"Euteneuer","year":"1985","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B22","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1126\/science.276.5309.118","article-title":"Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis","volume":"276","author":"Evangelista","year":"1997","journal-title":"Science"},{"key":"2023072900343410100_B23","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1083\/jcb.131.4.1003","article-title":"Yeast Num1p associates with the mother cell cortex during S\/G2 phase and affects microtubular functions","volume":"131","author":"Farkasovsky","year":"1995","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B24","doi-asserted-by":"crossref","first-page":"R414","DOI":"10.1016\/S0960-9822(06)00205-3","article-title":"Actin cytoskeleton: are FH proteins local organizers?","volume":"7","author":"Frazier","year":"1997","journal-title":"Curr Biol"},{"key":"2023072900343410100_B25","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1128\/jb.80.4.567-568.1960","article-title":"Bud formation in Saccharomyces cerevisiae.","volume":"80","author":"Freifelder","year":"1960","journal-title":"J Bacteriol"},{"key":"2023072900343410100_B26","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1091\/mbc.9.5.1221","article-title":"Rho1p-Bni1p-Spa2p interactions: implication in localization of Bni1p at the bud site and regulation of the actin cytoskeleton in Saccharomyces cerevisiae.","volume":"9","author":"Fujiwara","year":"1998","journal-title":"Mol Biol Cell"},{"key":"2023072900343410100_B27","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1101\/gad.12.3.396","article-title":"Cooperative interactions between the central spindle and the contractile ring during Drosophilacytokinesis","volume":"12","author":"Giansanti","year":"1998","journal-title":"Genes Dev"},{"key":"2023072900343410100_B28","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1083\/jcb.144.1.83","article-title":"Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons in yeast","volume":"144","author":"Goode","year":"1999","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B29","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1093\/emboj\/16.12.3474","article-title":"The Aspergillus nidulans sepAgene encodes an FH1\/2 protein involved in cytokinesis and the maintenance of cellular polarity","volume":"16","author":"Harris","year":"1997","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072900343410100_B30","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/S0960-9822(07)00539-8","article-title":"The role of Saccharomyces cerevisiaecoronin in the actin and microtubule cytoskeletons","volume":"8","author":"Heil-Chapdelaine","year":"1998","journal-title":"Curr Biol"},{"key":"2023072900343410100_B31","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1083\/jcb.109.3.1185","article-title":"Centrosome movement in the early divisions of Caenorhabditis elegans: a cortical site determining centrosome position","volume":"109","author":"Hyman","year":"1989","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B32","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1083\/jcb.105.5.2123","article-title":"Determination of cell division axes in the early embryogenesis of Caenorhabditis elegans.","volume":"105","author":"Hyman","year":"1987","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B33","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/0960-9822(92)90660-3","article-title":"Spindle positioning and cell polarity","volume":"2","author":"Hyman","year":"1992","journal-title":"Curr Biol"},{"key":"2023072900343410100_B34","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0092-8674(00)81285-4","article-title":"Morphogenic properties of microtubules and mitotic spindle assembly","volume":"84","author":"Hyman","year":"1996","journal-title":"Cell"},{"key":"2023072900343410100_B35","doi-asserted-by":"crossref","first-page":"2745","DOI":"10.1093\/emboj\/16.10.2745","article-title":"Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae.","volume":"16","author":"Imamura","year":"1997","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072900343410100_B36","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S0092-8674(00)81047-8","article-title":"Mother cell-specific HOexpression in budding yeast depends on the unconventional myosin Myo4p and other cytoplasmic proteins","volume":"84","author":"Jansen","year":"1996","journal-title":"Cell"},{"key":"2023072900343410100_B37","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1126\/science.275.5304.1311","article-title":"APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle","volume":"275","author":"Juang","year":"1997","journal-title":"Science"},{"key":"2023072900343410100_B38"},{"key":"2023072900343410100_B39","doi-asserted-by":"crossref","first-page":"9707","DOI":"10.1073\/pnas.92.21.9707","article-title":"Kinetics of spindle pole body separation in budding yeast","volume":"92","author":"Kahana","year":"1995","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072900343410100_B40","doi-asserted-by":"crossref","first-page":"28341","DOI":"10.1074\/jbc.273.43.28341","article-title":"Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p","volume":"273","author":"Kamei","year":"1998","journal-title":"J Biol Chem"},{"key":"2023072900343410100_B41","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1083\/jcb.142.1.181","article-title":"Targeting, capture, and stabilization of microtubules at early focal adhesions","volume":"142","author":"Kaverina","year":"1998","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B42","doi-asserted-by":"crossref","first-page":"6060","DOI":"10.1002\/j.1460-2075.1996.tb00994.x","article-title":"Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae.","volume":"15","author":"Kohno","year":"1996","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072900343410100_B43","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/383050a0","article-title":"Role of inscuteable in orienting asymmetric cell divisions in Drosophila.","volume":"383","author":"Kraut","year":"1996","journal-title":"Nature"},{"key":"2023072900343410100_B44","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1091\/mbc.8.10.1829","article-title":"The Rho-GEF Rom2p localizes to sites of polarized cell growth and participates in cytoskeletal functions in Saccharomyces cerevisiae.","volume":"10","author":"Manning","year":"1997","journal-title":"Mol Biol Cell"},{"key":"2023072900343410100_B45"},{"key":"2023072900343410100_B46","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1083\/jcb.140.2.377","article-title":"Kar9p is a novel cortical protein required for cytoplasmic microtubule orientation in yeast","volume":"140","author":"Miller","year":"1998","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B47","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1091\/mbc.9.8.2051","article-title":"The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast","volume":"9","author":"Miller","year":"1998","journal-title":"Mol Biol Cell"},{"key":"2023072900343410100_B48","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1093\/genetics\/145.3.671","article-title":"Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae.","volume":"145","author":"M\u00f6sch","year":"1997","journal-title":"Genetics"},{"key":"2023072900343410100_B49","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.1083\/jcb.109.6.3355","article-title":"The dynamics of chromosomal movement in the budding yeast Saccharomyces cerevisiae.","volume":"109","author":"Palmer","year":"1989","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B50","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1083\/jcb.119.3.583","article-title":"Role of astral microtubules and actin in spindle orientation and migration in the budding yeast, Saccharomyces cerevisiae.","volume":"119","author":"Palmer","year":"1992","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B51","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1083\/jcb.130.6.1373","article-title":"Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae.","volume":"130","author":"Pellman","year":"1995","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B52","doi-asserted-by":"crossref","first-page":"3697","DOI":"10.1128\/MCB.15.7.3697","article-title":"Characterization of fus1 of Schizosaccharomyces pombe: a developmentally controlled function needed for conjugation","volume":"15","author":"Petersen","year":"1995","journal-title":"Mol Cell Biol"},{"key":"2023072900343410100_B53","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S0091-679X(08)61620-9","article-title":"Fluorescence microscopy methods for yeast","volume":"31","author":"Pringle","year":"1989","journal-title":"Methods Cell Biol"},{"key":"2023072900343410100_B54","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0092-8674(95)90022-5","article-title":"Spindle orientation and asymmetric cell fate","volume":"82","author":"Rhyu","year":"1995","journal-title":"Cell"},{"key":"2023072900343410100_B55"},{"key":"2023072900343410100_B56","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1083\/jcb.139.4.985","article-title":"Astral microtubule dynamics in yeast: a microtubule-based searching mechanism for spindle orientation and nuclear migration into the bud","volume":"139","author":"Shaw","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B57","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1091\/mbc.9.7.1627","article-title":"Nuclear and spindle dynamics in budding yeast","volume":"9","author":"Shaw","year":"1998","journal-title":"Mol Biol Cell"},{"key":"2023072900343410100_B58","doi-asserted-by":"crossref","first-page":"4053","DOI":"10.1128\/MCB.18.7.4053","article-title":"Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis","volume":"18","author":"Sheu","year":"1998","journal-title":"Mol Cell Biol"},{"key":"2023072900343410100_B59","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/genetics\/122.1.19","article-title":"A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.","volume":"122","author":"Sikorski","year":"1989","journal-title":"Genetics"},{"key":"2023072900343410100_B60","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1083\/jcb.108.4.1419","article-title":"The SPA2protein of yeast localizes to sites of cell growth","volume":"108","author":"Snyder","year":"1989","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B61","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1083\/jcb.114.3.515","article-title":"Studies concerning the temporal and genetic control of cell polarity in Saccharomyces cerevisiae.","volume":"114","author":"Snyder","year":"1991","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B62","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0076-6879(91)94008-Z","article-title":"Assay of yeast mating reaction","volume":"194","author":"Sprague","year":"1991","journal-title":"Methods Enzymol"},{"key":"2023072900343410100_B63","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0955-0674(91)90163-S","article-title":"The plant cytoskeleton","volume":"3","author":"Staiger","year":"1991","journal-title":"Curr Opin Cell Biol"},{"key":"2023072900343410100_B64","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1083\/jcb.138.5.957","article-title":"Motoring to the finish: kinesin and dynein work together to orient the yeast mitotic spindle","volume":"138","author":"Stearns","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B65","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1126\/science.277.5325.574","article-title":"Mitosis in living budding yeast: anaphase A but no metaphase plate","volume":"277","author":"Straight","year":"1997","journal-title":"Science"},{"key":"2023072900343410100_B66","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1083\/jcb.135.3.725","article-title":"Pea2 protein of yeast is localized to sites of polarized growth and is required for efficient mating and bipolar budding","volume":"135","author":"Valtz","year":"1996","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B67","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1002\/yea.320101310","article-title":"New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.","volume":"10","author":"Wach","year":"1994","journal-title":"Yeast"},{"key":"2023072900343410100_B68","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1002\/(SICI)1097-0061(199704)13:5<399::AID-YEA126>3.0.CO;2-9","article-title":"Review: an overview of the Saccharomyces cerevisiaemicrotubule and microfilament cytoskeleton","volume":"13","author":"Winsor","year":"1997","journal-title":"Yeast"},{"key":"2023072900343410100_B69","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/346850a0","article-title":"\u201cFormins\u201d: proteins deduced from the alternative transcripts of the limb deformitygene","volume":"346","author":"Woychik","year":"1990","journal-title":"Nature"},{"key":"2023072900343410100_B70","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1083\/jcb.136.1.111","article-title":"A role for the actin cytoskeleton of Saccharomyces cerevisiaein bipolar bud-site selection","volume":"136","author":"Yang","year":"1997","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B71","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1083\/jcb.130.3.687","article-title":"Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae.","volume":"130","author":"Yeh","year":"1995","journal-title":"J Cell Biol"},{"key":"2023072900343410100_B72","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1128\/MCB.16.4.1857","article-title":"Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae.","volume":"16","author":"Zahner","year":"1996","journal-title":"Mol Cell Biol"},{"key":"2023072900343410100_B73","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1091\/mbc.4.12.1307","article-title":"Subcellular localization of Cdc42p, a Sacchamromyces cerevisiaeGTP-binding protein involved in the control of cell polarity","volume":"4","author":"Ziman","year":"1993","journal-title":"Mol Biol Cell"}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/144\/5\/947\/1850041\/9810022.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/144\/5\/947\/1850041\/9810022.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T00:35:32Z","timestamp":1690590932000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/144\/5\/947\/29449\/Control-of-Mitotic-Spindle-Position-by-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,3,8]]},"references-count":73,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1999,3,8]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.144.5.947","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,3,8]]}}}