{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T17:12:44Z","timestamp":1776445964610,"version":"3.51.2"},"reference-count":74,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,2,21]]},"abstract":"<jats:p>In this paper, we have analyzed the behavior of antibody cross-linked raft-associated proteins on the surface of MDCK cells. We observed that cross-linking of membrane proteins gave different results depending on whether cross-linking occurred on the apical or basolateral plasma membrane. Whereas antibody cross-linking induced the formation of large clusters on the basolateral membrane, resembling those observed on the surface of fibroblasts (Harder, T., P. Scheiffele, P. Verkade, and K. Simons. 1998. J. Cell Biol. 929\u2013942), only small (\u223c100 nm) clusters formed on the apical plasma membrane. Cross-linked apical raft proteins e.g., GPI-anchored placental alkaline phosphatase (PLAP), influenza hemagglutinin, and gp114 coclustered and were internalized slowly (\u223c10% after 60 min). Endocytosis occurred through surface invaginations that corresponded in size to caveolae and were labeled with caveolin-1 antibodies. Upon cholesterol depletion the internalization of PLAP was completely inhibited. In contrast, when a non-raft protein, the mutant LDL receptor LDLR-CT22, was cross-linked, it was excluded from the clusters of raft proteins and was rapidly internalized via clathrin-coated pits.<\/jats:p>\n               <jats:p>Since caveolae are normally present on the basolateral membrane but lacking from the apical side, our data demonstrate that antibody cross-linking induced the formation of caveolae, which slowly internalized cross-linked clusters of raft-associated proteins.<\/jats:p>","DOI":"10.1083\/jcb.148.4.727","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"727-740","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":103,"title":["Induction of Caveolae in the Apical Plasma Membrane of Madin-Darby Canine Kidney Cells"],"prefix":"10.1083","volume":"148","author":[{"given":"Paul","family":"Verkade","sequence":"first","affiliation":[{"name":"aEuropean Molecular Biology Laboratory, Cell Biology and Biophysics Programme, D-69117 Heidelberg, Germany"},{"name":"bMax Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany"}]},{"given":"Thomas","family":"Harder","sequence":"additional","affiliation":[{"name":"aEuropean Molecular Biology Laboratory, Cell Biology and Biophysics Programme, D-69117 Heidelberg, Germany"},{"name":"bMax Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany"}]},{"given":"Frank","family":"Lafont","sequence":"additional","affiliation":[{"name":"aEuropean Molecular Biology Laboratory, Cell Biology and Biophysics Programme, D-69117 Heidelberg, Germany"},{"name":"bMax Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany"}]},{"given":"Kai","family":"Simons","sequence":"additional","affiliation":[{"name":"aEuropean Molecular Biology Laboratory, Cell Biology and Biophysics Programme, D-69117 Heidelberg, Germany"},{"name":"bMax Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany"}]}],"member":"291","published-online":{"date-parts":[[1999,2,21]]},"reference":[{"key":"2023072902090134500_Ahmedetal1997","doi-asserted-by":"crossref","first-page":"10944","DOI":"10.1021\/bi971167g","article-title":"On the origin of sphingolipid\/cholesterol-rich detergent-insoluble cell membranesphysiological concentrations of cholesterol and sphingolipid induce formation of a detergent-insoluble, liquid-ordered lipid phase in model membranes","volume":"36","author":"Ahmed","year":"1997","journal-title":"Biochemistry."},{"key":"2023072902090134500_Arnietal1998","doi-asserted-by":"crossref","first-page":"28478","DOI":"10.1074\/jbc.273.43.28478","article-title":"Association of GAP-43 with detergent-resistant membranes requires two palmitoylated cysteine","volume":"273","author":"Arni","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072902090134500_N0x2f57db0N0x1e90bd0","doi-asserted-by":"crossref","first-page":"2687","DOI":"10.1002\/j.1460-2075.1984.tb02194.x","article-title":"Development of cell surface polarity in the epithelial Madin-Darby canine kidney (MDCK) cell line","volume":"3","author":"Balcarova-St\u00e4nder","year":"1984","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072902090134500_Bennettetal1988","doi-asserted-by":"crossref","first-page":"4075","DOI":"10.1002\/j.1460-2075.1988.tb03301.x","article-title":"Release of putative exocytic transport vesicles from perforated MDCK cells","volume":"7","author":"Bennett","year":"1988","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072902090134500_Bentingetal1999","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1083\/jcb.146.2.313","article-title":"N-glycans mediate the apical sorting of a GPI-anchored, raft-associated protein in Madin Darby canine kidney cells","volume":"146","author":"Benting","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_N0x2f57db0N0x1e90cf0","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1083\/jcb.111.6.2909","article-title":"Transcytosis in MDCK cellsidentification of glycoproteins transported bidirectionally between both plasma membrane domains","volume":"111","author":"Br\u00e4ndli","year":"1990","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Brownetal1989","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1126\/science.2571189","article-title":"Mechanism of membrane anchoring affects polarized expression of two proteins in MDCK cells","volume":"245","author":"Brown","year":"1989","journal-title":"Science."},{"key":"2023072902090134500_BrownandRose1992","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0092-8674(92)90189-J","article-title":"Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface","volume":"68","author":"Brown","year":"1992","journal-title":"Cell."},{"key":"2023072902090134500_BrownandLondon1997","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/bbrc.1997.7575","article-title":"Structure of detergent-resistant membrane domainsdoes phase separation occur in biological membranes?","volume":"240","author":"Brown","year":"1997","journal-title":"Biochem. Biophys. Res. Comm."},{"key":"2023072902090134500_BrownandLondon1998","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1146\/annurev.cellbio.14.1.111","article-title":"Functions of lipid rafts in biological membranes","volume":"14","author":"Brown","year":"1998","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"2023072902090134500_Casey1995","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1126\/science.7716512","article-title":"Protein lipidation in cell signaling","volume":"268","author":"Casey","year":"1995","journal-title":"Science."},{"key":"2023072902090134500_Cerneusetal1993","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1016\/S0021-9258(18)53671-1","article-title":"Detergent insolubility of alkaline phosphatase during biosynthetic transport and endocytosis. Role of cholesterol","volume":"268","author":"Cerneus","year":"1993","journal-title":"J. Biol Chem."},{"key":"2023072902090134500_DanielsenandvanDeurs1995","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1083\/jcb.131.4.939","article-title":"A transferrin-like GPI-linked iron-binding protein in detergent-insoluble non-caveolar microdomains at the apical surface of fetal intestinal epithelial cells","volume":"131","author":"Danielsen","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Deckertetal1996","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1083\/jcb.133.4.791","article-title":"Endocytosis of GPI-anchored proteins in human lymphocytesrole of glycolipid-based domains, actin cytoskeleton, and protein kinases","volume":"133","author":"Deckert","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Fiedleretal1993","doi-asserted-by":"crossref","first-page":"6365","DOI":"10.1021\/bi00076a009","article-title":"Glycosphingolipid-enriched detergent-insoluble complexes in protein sorting in epithelial cells","volume":"32","author":"Fiedler","year":"1993","journal-title":"Biochemistry."},{"key":"2023072902090134500_Fraetal1995","doi-asserted-by":"crossref","first-page":"8655","DOI":"10.1073\/pnas.92.19.8655","article-title":"De novo formation of caveolae in lymphocytes by expression of VIP21-caveolin","volume":"92","author":"Fra","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902090134500_FriedrichsonandKurzchalia1998","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1038\/29570","article-title":"Microdomains of GPI-anchored proteins in living cells revealed by chemical cross-linking","volume":"394","author":"Friedrichson","year":"1998","journal-title":"Nature."},{"key":"2023072902090134500_Fujimoto1996","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1177\/44.8.8756764","article-title":"GPI-anchored proteins, glycosphingolipids, and sphingomyelin are sequestered to caveolae only after crosslinking","volume":"44","author":"Fujimoto","year":"1996","journal-title":"J. Histochem. Cytochem."},{"key":"2023072902090134500_Geuzeetal1983","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/0092-8674(83)90518-4","article-title":"Intracellular site of asialoglycoprotein receptor-ligand uncouplingdouble-label immunoelectron microscopy during receptor-mediated endocytosis","volume":"32","author":"Geuze","year":"1983","journal-title":"Cell."},{"key":"2023072902090134500_Geuzeetal1987","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1083\/jcb.104.6.1715","article-title":"Membranes of sorting organelles display lateral heterogeneity in receptor distribution","volume":"104","author":"Geuze","year":"1987","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Gilbertetal1999","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1242\/jcs.112.7.1101","article-title":"Direct demonstration of the endocytic function of caveolae by a cell-free assay","volume":"112","author":"Gilbert","year":"1999","journal-title":"J. Cell Sci."},{"key":"2023072902090134500_Gottliebetal1993","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1083\/jcb.120.3.695","article-title":"Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells","volume":"120","author":"Gottlieb","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Hailstonesetal1998","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0022-2275(20)33898-0","article-title":"Regulation of caveolin and caveolae by cholesterol in MDCK cells","volume":"39","author":"Hailstones","year":"1998","journal-title":"J. Lipid Res."},{"key":"2023072902090134500_HarderandSimons1997","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/S0955-0674(97)80030-0","article-title":"Caveolae, DIGs, and the dynamics of sphingolipid-cholesterol microdomains","volume":"9","author":"Harder","year":"1997","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072902090134500_Harderetal1998","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1083\/jcb.141.4.929","article-title":"Lipid domain structure of the plasma membrane revealed by patching of membrane components","volume":"141","author":"Harder","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Jackmanetal1994","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1242\/jcs.107.9.2547","article-title":"Inhibition of apical but not basolateral endocytosis of ricin and folate in Caco-2 cells by cytochalasin D","volume":"107","author":"Jackman","year":"1994","journal-title":"J. Cell Sci."},{"key":"2023072902090134500_KellerandSimons1998","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1083\/jcb.140.6.1357","article-title":"Cholesterol is required for surface transport of influenza virus hemagglutinin","volume":"140","author":"Keller","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Lafontetal1998","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1083\/jcb.142.6.1413","article-title":"Annexin XIIIb associates with lipid microdomains to function in apical delivery","volume":"142","author":"Lafont","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_LeBivicetal1990","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1083\/jcb.110.5.1533","article-title":"Vectorial targeting of an endogenous apical membrane sialoglycoprotein and uvomorulin in MDCK cells","volume":"110","author":"Le Bivic","year":"1990","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_LeBivicetal1993","doi-asserted-by":"crossref","first-page":"6909","DOI":"10.1016\/S0021-9258(18)53126-4","article-title":"Ricin-resistant Madin-Darby canine kidney cells missort a major endogenous apical sialoglycoprotein","volume":"268","author":"Le Bivic","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023072902090134500_Lisantietal1994","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1083\/jcb.126.1.111","article-title":"Characterization of caveolin-rich membrane domains isolated from an endothelial-rich sourceimplications for human disease","volume":"126","author":"Lisanti","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_MatlinandSimons1983","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0092-8674(83)90154-X","article-title":"Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation","volume":"34","author":"Matlin","year":"1983","journal-title":"Cell."},{"key":"2023072902090134500_Matlinetal1981","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1083\/jcb.91.3.601","article-title":"Infectious entry pathway of influenza virus in a canine kidney cell line","volume":"91","author":"Matlin","year":"1981","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Matteretal1992","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/0092-8674(92)90551-M","article-title":"Basolateral sorting of LDL receptor in MDCK cellsthe cytoplasmic domain contains two tyrosine-dependent targeting determinants","volume":"71","author":"Matter","year":"1992","journal-title":"Cell."},{"key":"2023072902090134500_Mayoretal1994","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1126\/science.7516582","article-title":"Sequestration of GPI-anchored proteins in caveolae triggered by cross-linking","volume":"264","author":"Mayor","year":"1994","journal-title":"Science."},{"key":"2023072902090134500_Mayoretal1998","doi-asserted-by":"crossref","first-page":"4626","DOI":"10.1093\/emboj\/17.16.4626","article-title":"Cholesterol-dependent retention of GPI-anchored proteins in endosomes","volume":"17","author":"Mayor","year":"1998","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072902090134500_Melkonianetal1995","doi-asserted-by":"crossref","first-page":"16161","DOI":"10.1021\/bi00049a031","article-title":"Characterization of proteins in detergent-resistant membrane complexes from Madin-Darby canine kidney epithelial cells","volume":"34","author":"Melkonian","year":"1995","journal-title":"Biochemistry."},{"key":"2023072902090134500_Monieretal1995","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1091\/mbc.6.7.911","article-title":"VIP21-caveolin, a membrane protein constituent of the caveolar coat, oligomerizes in vivo and in vitro","volume":"6","author":"Monier","year":"1995","journal-title":"Mol. Biol. Cell."},{"key":"2023072902090134500_Murataetal1995","doi-asserted-by":"crossref","first-page":"10339","DOI":"10.1073\/pnas.92.22.10339","article-title":"VIP21\/caveolin is a cholesterol-binding protein","volume":"92","author":"Murata","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902090134500_Naimetal1995","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1083\/jcb.129.5.1241","article-title":"Apical and basolateral coated pits of MDCK cells differ in their rates of maturation into coated vesicles, but not in the ability to distinguish between mutant hemagglutinin proteins with different internalization signals","volume":"129","author":"Naim","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Partonetal1994","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1083\/jcb.127.5.1199","article-title":"Regulated internalization of caveolae","volume":"127","author":"Parton","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Parton1996","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/S0955-0674(96)80033-0","article-title":"Caveolae and caveolins","volume":"8","author":"Parton","year":"1996","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072902090134500_Petersetal1985","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1083\/jcb.101.6.2233","article-title":"Endothelial plasmalemmal vesicles have a characteristic striped bipolar surface structure","volume":"101","author":"Peters","year":"1985","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Pierinietal1996","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1083\/jcb.134.6.1427","article-title":"Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling","volume":"134","author":"Pierini","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Pimplikaretal1994","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1083\/jcb.125.5.1025","article-title":"Basolateral protein transport in streptolysin O-permeabilized MDCK cells","volume":"125","author":"Pimplikar","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Predescuetal1998","doi-asserted-by":"crossref","first-page":"6175","DOI":"10.1073\/pnas.95.11.6175","article-title":"Transcytosis of alpha1-acidic glycoprotein in the continuous microvascular endothelium","volume":"95","author":"Predescu","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902090134500_Resh1994","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/0092-8674(94)90104-X","article-title":"Myristylation and palmitylation of src family membersthe fats of the matter","volume":"76","author":"Resh","year":"1994","journal-title":"Cell."},{"key":"2023072902090134500_RietveldandSimons1998","first-page":"467","article-title":"The differential miscibility of lipids as the basis for the formation of functional membrane rafts","volume":"1376","author":"Rietveld","year":"1998","journal-title":"Biochem. Biophys. Acta."},{"key":"2023072902090134500_Rodeletal1999","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1091\/mbc.10.4.961","article-title":"Extraction of cholesterol with methyl-\u03b2-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles","volume":"10","author":"Rodel","year":"1999","journal-title":"Mol. Biol. Cell."},{"key":"2023072902090134500_Russelletal1984","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/0092-8674(84)90388-X","article-title":"Domain map of LDL receptorsequence homology with the epidermal growth factor precursor","volume":"37","author":"Russell","year":"1984","journal-title":"Cell."},{"key":"2023072902090134500_Sargiacomoetal1993","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1083\/jcb.122.4.789","article-title":"Signal transducing molecules and GPI-linked proteins form a caveolin-rich insoluble complex in MDCK cells","volume":"122","author":"Sargiacomo","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Scheiffeleetal1998","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1083\/jcb.140.4.795","article-title":"Caveolin-1 and -2 in the exocytic pathway of MDCK cells","volume":"140","author":"Scheiffele","year":"1998","journal-title":"J. Cell Biol"},{"key":"2023072902090134500_Schroederetal1994","doi-asserted-by":"crossref","first-page":"12130","DOI":"10.1073\/pnas.91.25.12130","article-title":"Interactions between saturated acyl chains confer detergent resistance on lipids and glycosylphosphatidylinositol (GPI)-anchored proteinsGPI-anchored proteins in liposomes and cells show similar behavior","volume":"91","author":"Schroeder","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902090134500_Schroederetal1998","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1074\/jbc.273.2.1150","article-title":"Cholesterol and sphingolipid enhance the Triton X-100 insolubility of glycosylphosphatidylinositol-anchored proteins by promoting the formation of detergent-insoluble ordered membrane domains","volume":"273","author":"Schroeder","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072902090134500_Sheetsetal1999","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1083\/jcb.145.4.877","article-title":"Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FcepsilonRI and their association with detergent-resistant membranes","volume":"145","author":"Sheets","year":"1999","journal-title":"J. Cell. Biol."},{"key":"2023072902090134500_Shuretyetal1996","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1242\/jcs.109.12.2927","article-title":"The effects of cytochalasin D and phorbol myristate acetate on the apical endocytosis of ricin in polarised Caco-2 cells","volume":"109","author":"Shurety","year":"1996","journal-title":"J. Cell Sci."},{"key":"2023072902090134500_Shuretyetal1998","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1091\/mbc.9.4.957","article-title":"Fluid-phase markers in the basolateral endocytic pathway accumulate in response to the actin assembly-promoting drug Jasplakinolide","volume":"9","author":"Shurety","year":"1998","journal-title":"Mol. Biol. Cell."},{"key":"2023072902090134500_SimonsandFuller1985","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1146\/annurev.cb.01.110185.001331","article-title":"Surface polarity in epithelia","volume":"1","author":"Simons","year":"1985","journal-title":"Ann. Rev. Cell Biol."},{"key":"2023072902090134500_SimonsandIkonen1997","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/42408","article-title":"Sphingolipid-cholesterol rafts in membrane trafficking and signalling","volume":"387","author":"Simons","year":"1997","journal-title":"Nature."},{"key":"2023072902090134500_SimonsandvanMeer1988","doi-asserted-by":"crossref","first-page":"6197","DOI":"10.1021\/bi00417a001","article-title":"Lipid sorting in epithelial cells","volume":"27","author":"Simons","year":"1988","journal-title":"Biochemistry."},{"key":"2023072902090134500_Skibbensetal1989","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1083\/jcb.108.3.821","article-title":"Differential extractability of influenza virus hemagglutinin during intracellular transport in polarized epithelial cells and nonpolar fibroblasts","volume":"108","author":"Skibbens","year":"1989","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_StahlandMueller1995","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1083\/jcb.129.2.335","article-title":"The urokinase-type plasminogen activator receptor, a GPI-linked protein, is localized in caveolae","volume":"129","author":"Stahl","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Stoorvogeletal1996","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1083\/jcb.132.1.21","article-title":"A novel class of clathrin-coated vesicles budding from endosomes","volume":"132","author":"Stoorvogel","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Subtiletal1999","doi-asserted-by":"crossref","first-page":"6775","DOI":"10.1073\/pnas.96.12.6775","article-title":"Acute cholesterol depletion inhibits clathrin-coated pit budding","volume":"96","author":"Subtil","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902090134500_Thomasetal1999","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1083\/jcb.125.4.795","article-title":"Large-scale co-aggregation of fluorescent lipid probes with cell surface proteins","volume":"125","author":"Thomas","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072902090134500_Trowbridgeetal1993","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev.cb.09.110193.001021","article-title":"Signal-dependent membrane protein trafficking in the endocytic pathway","volume":"9","author":"Trowbridge","year":"1993","journal-title":"Annu. Rev. Cell Biol."},{"key":"2023072902090134500_vanMeer1989","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1146\/annurev.cb.05.110189.001335","article-title":"Lipid traffic in animal cells","volume":"5","author":"van Meer","year":"1989","journal-title":"Ann. Rev. Cell Biol."},{"key":"2023072902090134500_VarmaandMayor1998","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1038\/29563","article-title":"GPI-anchored proteins occur in sub-micron domains at the surface of living cells","volume":"394","author":"Varma","year":"1998","journal-title":"Nature."},{"key":"2023072902090134500_VazandAlmeida1993","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/0959-440X(93)90071-R","article-title":"Phase topology in multi-phase lipid bilayersis the biological membrane a domain mosaic?","volume":"3","author":"Vaz","year":"1993","journal-title":"Curr. Opin. Struct. Biol."},{"key":"2023072902090134500_R489"},{"key":"2023072902090134500_N0x2f57db0N0x3a89d00","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s004180050161","article-title":"Lipid microdomains and membrane trafficking in mammalian cells","volume":"108","author":"Verkade","year":"1997","journal-title":"Histochem. Cell Biol."},{"key":"2023072902090134500_VerkadeandSimons1997","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1242\/jcs.111.6.825","article-title":"Expression of caveolin-1 and polarized formation of invaginated caveolae in Caco-2 and MDCK II cells","volume":"111","author":"Vogel","year":"1998","journal-title":"J. Cell Sci."},{"key":"2023072902090134500_Vogeletal1998","doi-asserted-by":"crossref","first-page":"3554","DOI":"10.1074\/jbc.272.6.3554","article-title":"Tyrosine kinase receptors concentrated in caveolae-like domains from neuronal plasma membrane","volume":"272","author":"Wu","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2023072902090134500_Wuetal1997","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1101\/SQB.1992.057.01.065","article-title":"Each caveola contains multiple glycosyl-phosphatidylinositol-anchored membrane proteins","volume":"57","author":"Ying","year":"1992","journal-title":"Cold Spring Harb. Symp. Quant. Biol."}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/148\/4\/727\/1857864\/9907066.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/148\/4\/727\/1857864\/9907066.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T02:10:08Z","timestamp":1690596608000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/148\/4\/727\/20460\/Induction-of-Caveolae-in-the-Apical-Plasma"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,2,21]]},"references-count":74,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,2,21]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.148.4.727","relation":{},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,2,21]]}}}