{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:40:20Z","timestamp":1773967220439,"version":"3.50.1"},"reference-count":79,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,3,6]]},"abstract":"<jats:p>Identification of signaling molecules that regulate cell migration is important for understanding fundamental processes in development and the origin of various pathological conditions. The migration of Nara Bladder Tumor II (NBT-II) cells was used to determine which signaling molecules are specifically involved in the collagen-mediated locomotion. We show here that paxillin is tyrosine phosphorylated after induction of motility on collagen. Overexpression of paxillin mutants in which tyrosine 31 and\/or tyrosine 118 were replaced by phenylalanine effectively impaired cell motility. Moreover, stimulation of motility by collagen preferentially enhanced the association of paxillin with the SH2 domain of the adaptor protein CrkII. Mutations in both tyrosine 31 and 118 diminished the phosphotyrosine content of paxillin and prevented the formation of the paxillin\u2013Crk complex, suggesting that this association is necessary for collagen-mediated NBT-II cell migration. Other responses to collagen, such as cell adhesion and spreading, were not affected by these mutations. Overexpression of wild-type paxillin or Crk could bypass the migration-deficient phenotype. Both the SH2 and the SH3 domains of CrkII are shown to play a critical role in this collagen-mediated migration. These results demonstrate the important role of the paxillin\u2013Crk complex in the collagen-induced cell motility.<\/jats:p>","DOI":"10.1083\/jcb.148.5.957","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:45:41Z","timestamp":1027701941000},"page":"957-970","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":232,"title":["Phosphorylation of Tyrosine Residues 31 and 118 on Paxillin Regulates Cell Migration through an Association with Crk in Nbt-II Cells"],"prefix":"10.1083","volume":"148","author":[{"given":"Val\u00e9rie","family":"Petit","sequence":"first","affiliation":[{"name":"aUMR 144, Centre National Recherche Scientifique, Institut Curie Section de Recherche, 26, rue d'Ulm, 75248, Paris Cedex 05, France"}]},{"given":"Brigitte","family":"Boyer","sequence":"additional","affiliation":[{"name":"aUMR 144, Centre National Recherche Scientifique, Institut Curie Section de Recherche, 26, rue d'Ulm, 75248, Paris Cedex 05, France"}]},{"given":"Delphine","family":"Lentz","sequence":"additional","affiliation":[{"name":"aUMR 144, Centre National Recherche Scientifique, Institut Curie Section de Recherche, 26, rue d'Ulm, 75248, Paris Cedex 05, France"}]},{"given":"Christopher E.","family":"Turner","sequence":"additional","affiliation":[{"name":"bDepartment of Anatomy and Cell Biology, State University of New York, Health Science Center, Syracuse, New York 13210"}]},{"given":"Jean Paul","family":"Thiery","sequence":"additional","affiliation":[{"name":"aUMR 144, Centre National Recherche Scientifique, Institut Curie Section de Recherche, 26, rue d'Ulm, 75248, Paris Cedex 05, France"}]},{"given":"Ana M.","family":"Vall\u00e9s","sequence":"additional","affiliation":[{"name":"aUMR 144, Centre National Recherche Scientifique, Institut Curie Section de Recherche, 26, rue d'Ulm, 75248, Paris Cedex 05, France"}]}],"member":"291","published-online":{"date-parts":[[2000,3,6]]},"reference":[{"key":"2023072902152468000_Angers-Loustauetal1999","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1083\/jcb.144.5.1019","article-title":"Protein tyrosine phosphatase-PEST regulates focal adhesion disassembly, migration, and cytokinesis in fibroblasts","volume":"144","author":"Angers-Loustau","year":"1999","journal-title":"J. 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J."},{"key":"2023072902152468000_Birchmeieretal1996","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1159\/000147848","article-title":"Epithelial\u2013mesenchymal transitions in cancer progression","volume":"156","author":"Birchmeier","year":"1996","journal-title":"Acta Anat."},{"key":"2023072902152468000_Birgeetal1993","first-page":"4648","article-title":"Identification and characterization of a high-affinity interaction between v-Crk and tyrosine-phosphorylated paxillin in CT10-transformed fibroblasts","volume":"13","author":"Birge","year":"1993","journal-title":"Mol. Cell. Biol."},{"key":"2023072902152468000_Boyeretal1989","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1083\/jcb.109.4.1495","article-title":"Rearrangements of desmosomal and cytoskeletal proteins during the transition from epithelial to fibroblastoid organization in cultured rat bladder carcinoma cells","volume":"109","author":"Boyer","year":"1989","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_Boyeretal1996","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1159\/000147849","article-title":"Model systems of epithelium-mesenchyme transitions","volume":"156","author":"Boyer","year":"1996","journal-title":"Acta Anat."},{"key":"2023072902152468000_Boyeretal1997","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.1093\/emboj\/16.19.5904","article-title":"Src and Ras are involved in separate pathways in epithelial cell scattering","volume":"16","author":"Boyer","year":"1997","journal-title":"EMBO (Eur. Mol. Biol. Organ.) J."},{"key":"2023072902152468000_Brand-Saberietal1996","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1006\/dbio.1996.0260","article-title":"Scatter factor\/hepatocyte growth factor (SF\/HGF) induces emigration of myogenic cells at interlimb level in vivo","volume":"179","author":"Brand-Saberi","year":"1996","journal-title":"Dev. Biol."},{"key":"2023072902152468000_Brownetal1996","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1083\/jcb.135.4.1109","article-title":"Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding","volume":"135","author":"Brown","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_Brownetal1998","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1091\/mbc.9.7.1803","article-title":"Serine and threonine phosphorylation of the paxillin LIM domains regulates paxillin focal adhesion localization and cell adhesion to fibronectin","volume":"9","author":"Brown","year":"1998","journal-title":"Mol. Biol. 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Cell Sci."},{"key":"2023072902152468000_Caryetal1998","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1083\/jcb.140.1.211","article-title":"Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration","volume":"140","author":"Cary","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_ClarkandBrugge1995","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1126\/science.7716514","article-title":"Integrins and signal transduction pathwaysthe road taken","volume":"268","author":"Clark","year":"1995","journal-title":"Science"},{"key":"2023072902152468000_deJongetal1997","doi-asserted-by":"crossref","first-page":"32649","DOI":"10.1074\/jbc.272.51.32649","article-title":"BCR\/ABL-induced leukemogenesis causes phosphorylation of Hef1 and its association with Crkl","volume":"272","author":"de Jong","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Giancotti1997","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/S0955-0674(97)80123-8","article-title":"Integrin signalingspecificity and control of cell survival and cell cycle progression","volume":"9","author":"Giancotti","year":"1997","journal-title":"Curr. Opin. Cell Biol"},{"key":"2023072902152468000_GilmoreandRomer1996","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1091\/mbc.7.8.1209","article-title":"Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreases cell motility and proliferation","volume":"7","author":"Gilmore","year":"1996","journal-title":"Mol. Biol. Cell"},{"key":"2023072902152468000_GlenneyandZokas1989","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1083\/jcb.108.6.2401","article-title":"Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton","volume":"108","author":"Glenney","year":"1989","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_Gumbiner1996","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0092-8674(00)81279-9","article-title":"Cell adhesionthe molecular basis of tissue architecture and morphogenesis","volume":"84","author":"Gumbiner","year":"1996","journal-title":"Cell"},{"key":"2023072902152468000_HanksandPolte1997","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/bies.950190208","article-title":"Signaling through focal adhesion kinase","volume":"19","author":"Hanks","year":"1997","journal-title":"Bioessays"},{"key":"2023072902152468000_Hasegawaetal1996","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1128\/MCB.16.4.1770","article-title":"DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane","volume":"16","author":"Hasegawa","year":"1996","journal-title":"Mol. Cell. 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Rev. Cell Dev. Biol"},{"key":"2023072902152468000_Keelyetal1997","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1038\/37656","article-title":"Cdc42 and Rac1 induce integrin-mediated cell motility and invasiveness through PI(3)K","volume":"390","author":"Keely","year":"1997","journal-title":"Nature"},{"key":"2023072902152468000_Keelyetal1998","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0962-8924(97)01219-1","article-title":"Integrins and GTPases in tumour cell growth, motility and invasion","volume":"8","author":"Keely","year":"1998","journal-title":"Trends Cell Biol."},{"key":"2023072902152468000_Khwajaetal1996","first-page":"2491","article-title":"Networks of interaction of p120Cbl and p130Cas with Crk and Grb2 adaptor proteins","volume":"12","author":"Khwaja","year":"1996","journal-title":"Oncogene."},{"key":"2023072902152468000_Kiyokawaetal1998a","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.1101\/gad.12.21.3331","article-title":"Activation of Rac1 by a Crk SH3-binding protein, DOCK180","volume":"12","author":"Kiyokawa","year":"1998","journal-title":"Genes Dev."},{"key":"2023072902152468000_Kiyokawaetal1998b","doi-asserted-by":"crossref","first-page":"24479","DOI":"10.1074\/jbc.273.38.24479","article-title":"Evidence that DOCK180 up-regulates signals from the CrkII-p130(Cas) complex","volume":"273","author":"Kiyokawa","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Klemkeetal1998","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1083\/jcb.140.4.961","article-title":"CAS\/Crk coupling serves as a molecular switch for induction of cell migration","volume":"140","author":"Klemke","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_Kochetal1991","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1126\/science.1708916","article-title":"SH2 and SH3 domainselements that control interactions of cytoplasmic signaling proteins","volume":"252","author":"Koch","year":"1991","journal-title":"Science"},{"key":"2023072902152468000_LewisandSchwartz1998","doi-asserted-by":"crossref","first-page":"14225","DOI":"10.1074\/jbc.273.23.14225","article-title":"Integrins regulate the association and phosphorylation of paxillin by c-Abl","volume":"273","author":"Lewis","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_MackayandHall1998","doi-asserted-by":"crossref","first-page":"20685","DOI":"10.1074\/jbc.273.33.20685","article-title":"Rho GTPases","volume":"273","author":"Mackay","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Matsudaetal1994","first-page":"5495","article-title":"CRK protein binds to two guanine nucleotide-releasing proteins for the Ras family and modulates nerve growth factor-induced activation of Ras in PC12 cells","volume":"14","author":"Matsuda","year":"1994","journal-title":"Mol. Cell. Biol."},{"key":"2023072902152468000_Matsudaetal1993","doi-asserted-by":"crossref","first-page":"4441","DOI":"10.1016\/S0021-9258(18)53628-0","article-title":"Structural requirement of CRK SH2 region for binding to phosphotyrosine-containing proteins. Evidence from reactivity to monoclonal antibodies","volume":"268","author":"Matsuda","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Mayeretal1995","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/S0960-9822(95)00060-1","article-title":"Evidence that SH2 domains promote processive phosphorylation by protein-tyrosine kinases","volume":"5","author":"Mayer","year":"1995","journal-title":"Curr. Biol."},{"key":"2023072902152468000_McCormacketal1997","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/sj.onc.1201186","article-title":"Activation of the focal adhesion kinase signal transduction pathway in cervical carcinoma cell lines and human genital epithelial cells immortalized with human papillomavirus type 18","volume":"15","author":"McCormack","year":"1997","journal-title":"Oncogene"},{"key":"2023072902152468000_Melamedetal1995","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1084\/jem.181.3.1071","article-title":"Nerve growth factor triggers microfilament assembly and paxillin phosphorylation in human B lymphocytes","volume":"181","author":"Melamed","year":"1995","journal-title":"J. Exp. Med."},{"key":"2023072902152468000_Miyamotoetal1995","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1126\/science.7846531","article-title":"Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function","volume":"267","author":"Miyamoto","year":"1995","journal-title":"Science"},{"key":"2023072902152468000_Nakashimaetal1999","doi-asserted-by":"crossref","first-page":"3001","DOI":"10.1074\/jbc.274.5.3001","article-title":"The functional role of CrkII in actin cytoskeleton organization and mitogenesis","volume":"274","author":"Nakashima","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Nojimaetal1995","doi-asserted-by":"crossref","first-page":"15398","DOI":"10.1074\/jbc.270.25.15398","article-title":"Integrin-mediated cell adhesion promotes tyrosine phosphorylation of p130Cas, a Src homology 3-containing molecule having multiple Src homology 2-binding motifs","volume":"270","author":"Nojima","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Petitetal1999","doi-asserted-by":"crossref","first-page":"151","DOI":"10.3109\/15419069909010799","article-title":"Characterization of the signaling pathways regulating \u03b12\u03b21 integrin-mediated events by a pharmacological approach","volume":"7","author":"Petit","year":"1999","journal-title":"Cell Adhes. Commun."},{"key":"2023072902152468000_RankinandRozengurt1994","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/S0021-9258(17)42406-9","article-title":"Platelet-derived growth factor modulation of focal adhesion kinase (p125FAK) and paxillin tyrosine phosphorylation in Swiss 3T3 cells. Bell-shaped dose response and cross-talk with bombesin","volume":"269","author":"Rankin","year":"1994","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Rodieretal1995","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1083\/jcb.131.3.761","article-title":"pp60c-src is a positive regulator of growth factor-induced cell scattering in a rat bladder carcinoma cell line","volume":"131","author":"Rodier","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_Sabeetal1994","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.1073\/pnas.91.9.3984","article-title":"Analysis of the binding of the Src homology 2 domain of Csk to tyrosine-phosphorylated proteins in the suppression and mitotic activation of c-Src","volume":"91","author":"Sabe","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072902152468000_Sabeetal1995","doi-asserted-by":"crossref","first-page":"31219","DOI":"10.1074\/jbc.270.52.31219","article-title":"Possible v-Crk-induced transformation through activation of Src kinases","volume":"270","author":"Sabe","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Salgiaetal1995","doi-asserted-by":"crossref","first-page":"29145","DOI":"10.1074\/jbc.270.49.29145","article-title":"CRKL links p210BCR\/ABL with paxillin in chronic myelogenous leukemia cells","volume":"270","author":"Salgia","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Salgiaetal1996","doi-asserted-by":"crossref","first-page":"31222","DOI":"10.1074\/jbc.271.49.31222","article-title":"The related adhesion focal tyrosine kinase forms a complex with paxillin in hematopoietic cells","volume":"271","author":"Salgia","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Sanderetal1998","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1083\/jcb.143.5.1385","article-title":"Matrix-dependent Tiam1\/Rac signaling in epithelial cells promotes either cell\u2013cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase","volume":"143","author":"Sander","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_SchallerandParsons1995","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1128\/MCB.15.5.2635","article-title":"pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk","volume":"15","author":"Schaller","year":"1995","journal-title":"Mol. Cell. Biol."},{"key":"2023072902152468000_SchoenwaelderandBurridge1999","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/S0955-0674(99)80037-4","article-title":"Bidirectional signaling between the cytoskeleton and integrins","volume":"11","author":"Schoenwaelder","year":"1999","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072902152468000_Schor1994","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0955-2235(94)90007-8","article-title":"Cytokine control of cell motilitymodulation and mediation by the extracellular matrix","volume":"5","author":"Schor","year":"1994","journal-title":"Prog. Growth Factor Res."},{"key":"2023072902152468000_Schwartz1997","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1083\/jcb.139.3.575","article-title":"Integrins, oncogenes, and anchorage independence","volume":"139","author":"Schwartz","year":"1997","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_Schwartzetal1996","doi-asserted-by":"crossref","first-page":"6525","DOI":"10.1002\/j.1460-2075.1996.tb01043.x","article-title":"Transformation by Rho exchange factor oncogenes is mediated by activation of an integrin-dependent pathway","volume":"15","author":"Schwartz","year":"1996","journal-title":"EMBO (Eur. Mol. Biol. Organ.) 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Cancer Inst."},{"key":"2023072902152468000_Tuckeretal1990","first-page":"129","article-title":"Collagen-mediated dispersion of NBT-II rat bladder carcinoma cells","volume":"50","author":"Tucker","year":"1990","journal-title":"Cancer Res."},{"key":"2023072902152468000_Turner1998","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/S1357-2725(98)00062-4","article-title":"Paxillin","volume":"30","author":"Turner","year":"1998","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"2023072902152468000_TurnerandMiller1994","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1242\/jcs.107.6.1583","article-title":"Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domainsidentification of a vinculin and pp125Fak-binding region","volume":"107","author":"Turner","year":"1994","journal-title":"J. Cell Sci."},{"key":"2023072902152468000_Turneretal1990","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1083\/jcb.111.3.1059","article-title":"Paxillina new vinculin-binding protein present in focal adhesions","volume":"111","author":"Turner","year":"1990","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_Turneretal1995","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1242\/jcs.108.1.333","article-title":"Angiotensin II stimulation of rapid paxillin tyrosine phosphorylation correlates with the formation of focal adhesions in rat aortic smooth muscle cells","volume":"108","author":"Turner","year":"1995","journal-title":"J. Cell Sci."},{"key":"2023072902152468000_Turneretal1999","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1083\/jcb.145.4.851","article-title":"Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP proteina role in cytoskeletal remodeling","volume":"145","author":"Turner","year":"1999","journal-title":"J. Cell Biol."},{"key":"2023072902152468000_N0x28dbbd0N0x3bd0280","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1091\/mbc.1.13.975","article-title":"Alternative patterns of mitogenesis and cell scattering induced by acidic FGF as a function of cell density in a rat bladder carcinoma cell line","volume":"1","author":"Vall\u00e9s","year":"1990","journal-title":"Cell Regul."},{"key":"2023072902152468000_N0x28dbbd0N0x3bd02e0","first-page":"232","article-title":"In vitro studies of epithelium-to-mesenchyme transitions","volume-title":"Cell Biology. A Laboratory Handbook. Vol. 1","author":"Vall\u00e9s","year":"1994"},{"key":"2023072902152468000_N0x28dbbd0N0x3bd0340","doi-asserted-by":"crossref","first-page":"187","DOI":"10.3109\/15419069609014222","article-title":"\u03b12\u03b21 integrin is required for the collagen and FGF-1 induced cell dispersion in a rat bladder carcinoma cell line","volume":"4","author":"Vall\u00e9s","year":"1996","journal-title":"Cell Adhes. Commun."},{"key":"2023072902152468000_VandePoletal1998","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/sj.onc.1201504","article-title":"Association of bovine papillomavirus type 1 E6 oncoprotein with the focal adhesion protein paxillin through a conserved protein interaction motif","volume":"16","author":"Vande Pol","year":"1998","journal-title":"Oncogene"},{"key":"2023072902152468000_Viebahn1995","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1159\/000147753","article-title":"Epithelio\u2013mesenchymal transformation during formation of the mesoderm in the mammalian embryo","volume":"154","author":"Viebahn","year":"1995","journal-title":"Acta Anat."},{"key":"2023072902152468000_Wengetal1993","doi-asserted-by":"crossref","first-page":"14956","DOI":"10.1016\/S0021-9258(18)82425-5","article-title":"Detection of Src homology 3-binding proteins, including paxillin, in normal and v-Src-transformed Balb\/c 3T3 cells","volume":"268","author":"Weng","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Wuetal1998","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1074\/jbc.273.1.528","article-title":"Integrin-linked protein kinase regulates fibronectin matrix assembly, E-cadherin expression, and tumorigenicity","volume":"273","author":"Wu","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Yuetal1998","doi-asserted-by":"crossref","first-page":"21125","DOI":"10.1074\/jbc.273.33.21125","article-title":"Protein-tyrosine phosphatase Shp-2 regulates cell spreading, migration, and focal adhesion","volume":"273","author":"Yu","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072902152468000_Zacharyetal1993","doi-asserted-by":"crossref","first-page":"22060","DOI":"10.1016\/S0021-9258(20)80648-6","article-title":"Bombesin, vasopressin, and endothelin rapidly stimulate tyrosine phosphorylation of the focal adhesion-associated protein paxillin in Swiss 3T3 cells","volume":"268","author":"Zachary","year":"1993","journal-title":"J. Biol. 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