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Using synthetic peptides, we find that the cytoplasmic domain of p24\u03b21 can bind Arf GTPase-activating protein (GAP)1 and cause direct inhibition of ArfGAP1-mediated GTP hydrolysis on Arf-1 bound to liposomes and Golgi membranes. We propose a two-stage reaction to explain how GTP hydrolysis constitutes a prerequisite for sorting of resident proteins, yet becomes inhibited in their presence.<\/jats:p>","DOI":"10.1083\/jcb.200108017","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:46:23Z","timestamp":1027687583000},"page":"1199-1212","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":154,"title":["Sorting of Golgi resident proteins into different subpopulations of COPI vesicles"],"prefix":"10.1083","volume":"155","author":[{"given":"Joel","family":"Lanoix","sequence":"first","affiliation":[{"name":"1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D-69017 Heidelberg, Germany"}]},{"given":"Joke","family":"Ouwendijk","sequence":"additional","affiliation":[{"name":"1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D-69017 Heidelberg, Germany"}]},{"given":"Annika","family":"Stark","sequence":"additional","affiliation":[{"name":"1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D-69017 Heidelberg, Germany"}]},{"given":"Edith","family":"Szafer","sequence":"additional","affiliation":[{"name":"2Department of Biology, Technion, Haifa 32000, Israel"}]},{"given":"Dan","family":"Cassel","sequence":"additional","affiliation":[{"name":"2Department of Biology, Technion, Haifa 32000, Israel"}]},{"given":"Kurt","family":"Dejgaard","sequence":"additional","affiliation":[{"name":"1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D-69017 Heidelberg, Germany"}]},{"given":"Matthias","family":"Weiss","sequence":"additional","affiliation":[{"name":"1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D-69017 Heidelberg, Germany"}]},{"given":"Tommy","family":"Nilsson","sequence":"additional","affiliation":[{"name":"1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D-69017 Heidelberg, Germany"}]}],"member":"291","published-online":{"date-parts":[[2001,12,17]]},"reference":[{"key":"2023072904453657600_BIB1","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/35078500","volume":"3","year":"2001","journal-title":"Nat. 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