{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:28:12Z","timestamp":1772137692214,"version":"3.50.1"},"reference-count":48,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,12,24]]},"abstract":"<jats:p>Procollagen (PC)-I aggregates transit through the Golgi complex without leaving the lumen of Golgi cisternae. Based on this evidence, we have proposed that PC-I is transported across the Golgi stacks by the cisternal maturation process. However, most secretory cargoes are small, freely diffusing proteins, thus raising the issue whether they move by a transport mechanism different than that used by PC-I. To address this question we have developed procedures to compare the transport of a small protein, the G protein of the vesicular stomatitis virus (VSVG), with that of the much larger PC-I aggregates in the same cell. Transport was followed using a combination of video and EM, providing high resolution in time and space. Our results reveal that PC-I aggregates and VSVG move synchronously through the Golgi at indistinguishable rapid rates. Additionally, not only PC-I aggregates (as confirmed by ultrarapid cryofixation), but also VSVG, can traverse the stack without leaving the cisternal lumen and without entering Golgi vesicles in functionally relevant amounts. Our findings indicate that a common mechanism independent of anterograde dissociative carriers is responsible for the traffic of small and large secretory cargo across the Golgi stack.<\/jats:p>","DOI":"10.1083\/jcb.200108073","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:46:23Z","timestamp":1027687583000},"page":"1225-1238","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":181,"title":["Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae"],"prefix":"10.1083","volume":"155","author":[{"given":"Alexander A.","family":"Mironov","sequence":"first","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Galina V.","family":"Beznoussenko","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Paolo","family":"Nicoziani","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Oliviano","family":"Martella","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Alvar","family":"Trucco","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Hee-Seok","family":"Kweon","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Daniele","family":"Di Giandomenico","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Roman S.","family":"Polishchuk","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Aurora","family":"Fusella","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]},{"given":"Pietro","family":"Lupetti","sequence":"additional","affiliation":[{"name":"2Dipartimento di Biologia Evolutiva, Universita\u0300 di Siena, 453100 Siena, Italy"}]},{"given":"Eric G.","family":"Berger","sequence":"additional","affiliation":[{"name":"3Institute of Physiology, University of Zurich, CH-8057 Zurich, Switzerland"}]},{"given":"Willie J.C.","family":"Geerts","sequence":"additional","affiliation":[{"name":"4Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, 83584 CH Utrecht, Netherlands"}]},{"given":"Abraham J.","family":"Koster","sequence":"additional","affiliation":[{"name":"4Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, 83584 CH Utrecht, Netherlands"}]},{"given":"Koert N.J.","family":"Burger","sequence":"additional","affiliation":[{"name":"4Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, 83584 CH Utrecht, Netherlands"}]},{"given":"Alberto","family":"Luini","sequence":"additional","affiliation":[{"name":"1Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche \u201cMario Negri,\u201d 66030 Santa Maria Imbaro, Chieti, Italy"}]}],"member":"291","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"2023072908054478000_BIB1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1083\/jcb.138.1.1","volume":"138","year":"1997","journal-title":"J. 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